Ribo-Zero Plus Microbiome rRNA Depletion Kit

Fast, efficient depletion of undesirable host and pan-bacterial rRNA from complex microbial samples (eg stool) for metatranscriptomics research. Read More...
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Illumina® Ribo-Zero Plus rRNA Microbiome depletion kit (96 Samples)

20072062

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Illumina Stranded Total RNA Library Prep with Ligation, Ribo-Zero Plus Microbiome Depletion Kit (96 samples)

20072063

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Product Highlights

The Illumina Ribo-Zero Plus Microbiome rRNA Depletion Kit is a streamlined RNA-to-analysis solution for metatranscriptome sequencing of complex microbial samples, including stool.

  • Perform deeper analysis and gain insights into microbial activity by reducing unwanted reads and increasing coverage of expressed bacterial RNA.
  • Eliminate unwanted rRNA from bacteria common in the adult and infant human gut, as well as host RNA from human, mouse, using proven Ribo-Zero Plus enzymatic ribodepletion technology.
  • Analyze complex metatranscriptomes with complimentary access to the BaseSpace Microbiome Metatranscriptomics app.

Read the data sheet to learn more about the metatranscriptomics applications.

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Specifications

Product Comparison

  Ribo-Zero Plus Microbiome rRNA Depletion Kit Ribo-Zero Plus rRNA Depletion Kit
Description Depletion of abundant transcripts from host and bacterial rRNA for metatranscriptomics research. Single-tube depletion of abundant transcripts from multiple species or transcripts
Input Quantity Minimum 25 ng of total RNA, 50 ng recommended for optimal performance 1-1000 ng total RNA for standard-quality RNA samples. 10ng total RNA minimum recommended for optimal performance and FFPE samples
System Compatibility Details Library prep is designed to be compatible with all lllumina sequencing systems, and is recommended to be used with NextSeq 500, NextSeq 550, NextSeq 1000, NextSeq 2000, and NovaSeq 6000 Systems. Library prep is designed to be compatible with all lllumina sequencing systems, and is extensively validated on the NextSeq 500, NextSeq 550 and NovaSeq 6000 Systems.
Strand Specificity Stranded Stranded
Method Whole-Transcriptome Sequencing, Metatranscriptome Sequencing Whole-Transcriptome Sequencing
Variant Class Novel Transcripts, Transcript Variants Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants
Species Category Bacteria, Human, Mouse, Rat Bacteria, Human, Mouse, Rat
Species Details Depletion of abundant rRNA transcripts from complex bacterial (microbiome) samples including stool samples, in addition to all transcripts listed in Ribo-Zero Plus. Depletion of abundant transcripts from multiple species including: human cytoplasmic & mitochondria rRNA, mouse rRNA, rat rRNA, E. coli and B. subtilis rRNA, human beta globin transcripts
System Compatibility NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000 HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000
Specialized Sample Types Microbiome Samples FFPE Tissue, Low-Input Samples
Nucleic Acid Type RNA RNA

Method-Specific Workflow Example

 

Supporting Data and Figures

 
Metatranscriptomics: Challenges and Opportunities

In this webinar, you'll hear from three leading experts in the field as they share their insights and key considerations on sample collection and processing, rRNA depletion, and bioinformatic analysis.

Watch Webinar
watch the Metatranscriptomics webinar

Manuals and Support Information

A dedicated support section is not currently available for this product

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