This targeted RNA sequencing panel is a cost-effective solution to detect gene fusions in multiple cancer types, regardless of origin. Covering 507 fusion-associated genes, a single assay enables researchers to assess most known cancer-related fusions in blood, bone marrow, and FFPE samples, with the power to identify novel fusion gene partners.
On-instrument fusion calling provides NGS-based fusion detection to labs without additional bioinformatics support. The analysis provides a list of detected fusions, relevant disease associations (as identified by the Mitelman database), and evidence of fusion-supporting reads.
This assay accommodates as little input as 20 ng FFPE RNA or 10 ng fresh-frozen total RNA. The TruSight RNA Fusion Panel provides a sensitive, reproducible, and economical solution for studies of gene fusions in cancer research.
Instrument | Recommended Number of Samples | Read Length |
---|---|---|
MiniSeq System | Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample) | 2 × 76 bp (max recommended) |
MiSeq System | 8 samples per run with v3 reagents (based on 3 million reads per sample) | 2 × 76 bp (max recommended) |
NextSeq 550 System | 24 samples per run (mid output; based on 3 million reads per sample) | 2 × 76 bp (max recommended) |
TruSight RNA Fusion Panel | TruSight Tumor 170 | AmpliSeq for Illumina Focus Panel | AmpliSeq for Illumina Comprehensive Panel v3 | |
---|---|---|---|---|
Assay Time | 2.5 days | ~2 days (Library Prep) | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
Cancer Type | Pan-Cancer | Solid Tumor | Solid Tumor | Solid Tumor |
Hands-On Time | 11 hours | ~10.5 hours | < 1.5 hours | < 1.5 hours |
Method | Target Enrichment, Targeted RNA Sequencing | Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing |
Nucleic Acid Type | RNA | RNA, DNA | DNA, RNA | DNA, RNA |
Specialized Sample Types | FFPE Tissue, Low-Input Samples | FFPE Tissue, Low-Input Samples | FFPE Tissue | FFPE Tissue |
Species Category | Human | Human | Human | Human |
TruSight RNA Fusion Panel Gene List
product_file | EXCEL < 1 MB
Brochure | PDF 6 MB
Data Sheet | PDF 1 MB
Illumina Experiment Manager User Guide Documentation
Sequencing Library qPCR Quantification Guide Documentation
Local Run Manager RNA Fusion Analysis Module Workflow Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All TruSight RNA Fusion Panel Documentation Support All TruSight RNA Fusion Panel Support