Nextera Rapid Capture Custom Enrichment Kit
Rapidly interrogate those portions of the human genome most important to your specific research.Read More...
Select Product(s)
Services
Total:
Nextera Rapid Capture Custom Enrichment provides focused access to genomic regions of interest. Leverage this all-in-one library preparation and enrichment workflow, and design your preferred assay with a user-friendly online tool.
This custom assay allows researchers to sequence precious samples quickly and efficiently, using as little as 50 ng of input DNA. The kit offers add-on functionality to refine content over time, or add regions of unique interest to established panels such as TruSight content sets.
With Nextera Rapid Capture Custom Enrichment, you can rapidly interrogate those portions of the human genome most important to your project. Densely pack hybridization probes into a region as small as 0.5 Mb, or space them more liberally to enrich up to 15 Mb of fully custom content. This assay offers maximum flexibility to address specific needs.
Add-on functionality allows you to periodically increase those regions of the genome enriched by the custom assay. You can start from our pre-defined panels and add in regions of particular interest, or start from an existing custom design and capitalize on new discoveries. For example, a project can start with existing exome content and add in important non-coding content.
Find automation vendors who offer robotic systems compatible with this kit
DesignStudio Software is an intuitive and fully supported online tool for designing custom assays. This free tool is available to anyone with a MyIllumina account. Add targets or regions of interest, optimize probe density, explore other design options, and estimate your project cost, all before committing to a final order.
Start a DesignStudio Project (customer login required)
| Nextera Rapid Capture Custom Enrichment Kit | Illumina DNA Prep with Enrichment | AmpliSeq for Illumina Custom DNA Panel | AmpliSeq for Illumina On-Demand | |
|---|---|---|---|---|
| Content Specifications | Custom genomic content of interest (0.5 - 15 Mb) | Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Custom: 0.5 - 15 Mb genomic content of interest. Fixed panels: Content varies by panel. |
Custom content of interest - up to 5 Mb | Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes |
| Description | A custom research workflow that utilizes custom panels, existing Illumina designed panels or previously ordered custom projects to enable enrichment of targeted genomic content. | A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing. | A custom research assay that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. | A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. |
| Input Quantity | 50 ng DNA | 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide). | 1–100 ng (10 ng recommended per pool) | 1-100 ng DNA |
| Mechanism of Action | Enzymatic fragmentation and hybridization-based enrichment | Bead-bound transposomes and hybrid-capture chemistry | Multiplex PCR | Multiplex PCR |
| Method | Custom Sequencing, Target Enrichment , Targeted DNA Sequencing | Custom Sequencing, Exome Sequencing , Target Enrichment , Targeted DNA Sequencing | Amplicon Sequencing , Custom Sequencing, Genotyping by Sequencing , Targeted DNA Sequencing | Amplicon Sequencing , Targeted DNA Sequencing |
| Multiplexing | 12-plex (pool up to 12 samples per enrichment reaction) | Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. | Up to 96-plex | Up to 96-plex |
| Number of Reactions | 48, 96, or 288 samples | Custom workflow: 16 or 96 samples | 750 or 3000 reactions | 24 or 96 reactions |
| Specialized Sample Types | Not FFPE-Compatible | Blood, FFPE Tissue, Saliva | Blood, FFPE Tissue | Blood, FFPE Tissue |
| Species Category | Human | Human, Other | Any Species, Bovine, Canine, Chicken, Hamster, Human, Maize, Mouse, Ovine, Porcine, Rice, Soybean, Tomato | Human |
Franco Taroni, MD, uses broad gene panels and the Nextera Rapid Capture Custom Enrichment Kit to study metabolic and neurological disorders.
Learn MoreA novel QC approach and the Nextera Rapid Capture Exome Kit enable researchers to sequence degraded DNA from FFPE tumor samples successfully.
Learn More
Nextera Rapid Capture Custom Enrichment provides uniform target enrichment across different custom probe sets and individual samples within a 12-plex pool. A. Coverage uniformity is shown as % of targeted bases represented by >0.2x mean coverage. Error bars show standard deviation of uniformity across the 12 pooled samples for each project. B. Coverage uniformity for each of 12 pooled samples within Project 3 is shown. Mean coverage for this run was 300x, and % of targeted bases that were covered at >60x are shown.
Nextera Flex products provide time-saving solutions for a wide range of sequencing applications. The unique bead-linked transposome chemistry integrates DNA extraction, fragmentation, library preparation, and library normalization steps to deliver the fastest, most flexible workflow in the Illumina library prep portfolio.
Learn More
Nextera Rapid Capture Enrichment Low-Plex Pooling Guidelines
Technical Note | PDF< 1 MB
Nextera Rapid Capture Custom Enrichment Data Sheet,Nextera Rapid Capture Custom Enrichment
Data Sheet | PDF< 1 MB
Nextera Rapid Capture Enrichment Reference Guide Documentation
Nextera Rapid Capture Enrichment User-Supplied Consumables and Equipment Documentation
Illumina Experiment Manager User Guide Documentation
Nextera Rapid Capture Enrichment Sequencing Product Insert Documentation
Sequencing Library qPCR Quantification Guide Documentation
HiSeq Analysis Software v2.1 Release Notes Documentation
HiSeq Analysis Software User Guide Documentation
HiSeq Analysis Software v2.1 User Guide Documentation
Nextera Rapid Capture Enrichment Consumables & Equipment List Documentation
Nextera Rapid Capture Enrichment Checklist Documentation
Nextera Rapid Capture Custom Enrichment Kit Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.
This exome sequencing library preparation solution uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome.
A fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole-exome enrichment.
