For high-throughput research, bead-based normalization can save time and resources by providing an accelerated path from DNA to data. This technical note provides guidelines and data comparing the two normalization methods to help you decide which option to use.Read Tech Note
|Nextera XT DNA Library Preparation Kit||Illumina DNA Prep|
|Assay Time||~5.5 hours from DNA extraction to normalized library. (Library prep time: ~90 minutes).||~3-4 hours (from DNA extraction to normalized library)|
|Description||Fast library prep optimized for research on small genomes, PCR amplicons, and plasmids.||A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more.|
|Hands-On Time||15 minutes||1-1.5 hours|
|Input Quantity||1 ng DNA||Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide).|
|Mechanism of Action||Enzymatic fragmentation||Bead-linked transposome|
|Method||16s rRNA Sequencing, Amplicon Sequencing, De Novo Sequencing, Shotgun Sequencing, Whole-Genome Sequencing||Amplicon Sequencing, De Novo Sequencing, Shotgun Sequencing, Whole-Genome Sequencing|
|Multiplexing||Up to 384 uniquely indexed samples may be pooled and sequenced together.||Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations|
|Specialized Sample Types||Low-Input Samples, Not FFPE-Compatible, Single Cells||Blood, Not FFPE-Validated, Saliva|
|Species Details||Compatible with any species||Compatible with any species|
|Target Insert Size||300 bp–1.5 kb||~350bp|
Single-cell mRNA-Seq with the MiSeq and HiSeq Systems is enabling Allen Institute researchers to classify V1 neural cells.Read More
Genomic studies with the HiSeq and MiSeq Systems are enabling researchers to track Ebola outbreaks and understand the impact of the virus’s rapid mutation rate.Read More
The combination of Nextera XT and rapid sequencing with the MiSeq System provides a comprehensive DNA to data workflow in only 8 hours.
The Nextera XT Library Preparation Kit eliminates the need for library quantification before sample pooling and sequencing. Libraries of equivalent concentrations are created by employing bead-based sample normalization, as simple as pipetting 5 μl of each library to be sequenced.