Infinium MethylationEPIC Kit

The Infinium MethylationEPIC kit provides unparalleled coverage of CpG islands, genes, and enhancers. Infinium HD array technology enables content selection independent of bias-associated limitations often associated with methylated DNA capture methods.

The result is a pan-enhancer and coding region view of the methylome that can be used for epigenome-wide association studies on a variety of human tissues. It includes the following content categories requested by methylation experts:

  • CpG sites outside of CpG islands
  • Non-CpG methylated sites identified in human stem cells (CHH sites)
  • Differentially methylated sites identified in tumor versus normal
  • FANTOM5 enhancers
  • ENCODE open chromatin and enhancers
  • DNase hypersensitive sites
  • miRNA promoter regions
  • > 90% of content contained on the Illumina HumanMethylation450K BeadChip

Seamless Synergy with TruSeq Methyl Capture EPIC

In early 2016, Illumina will launch the TruSeq Methyl Capture EPIC panel, an enrichment-based bisulfite sequencing method that mirrors and expands upon the content of the MethylationEPIC chip. Now researchers can move seamlessly between array and sequencing technologies. They can take advantage of the low price point on the MethylationEPIC chip for large-scale screens and dive deep on specific samples or subsets with the TruSeq Methyl Capture EPIC panel.

Integrated Analysis Software

MethylationEPIC data analysis is supported by the GenomeStudio Methylation Module. GenomeStudio software enables researchers to effortlessly perform differential methylation analysis for small-scale studies. It features advanced visualization tools that enable researchers to view vast amounts of data in a single graph, such as heat maps, scatter plots, and line plots. These tools and the GenomeStudio Genome Browser display valuable information such as chromosomal coordinates, percent GC, location in a CpG Island, and methylation β values.

For large-scale studies, there are many free analysis packages that work in the software framework R for normalization, and differential analysis of methylation data. Instructional videos explain the use of these data analysis packages.

Product Literature

Description Document Type File Info
Infinium MethylationEPIC BeadChip Data Sheet | Illumina Data Sheet HTML

Catalog IDs: WG-317-1001, WG-317-1002, WG-317-1003