Nextera Mate Pair Sample Preparation Kit

The Nextera Mate Pair Sample Preparation Kit offers the only gel-free method for preparing up to 12 kb mate pair libraries with the industry’s lowest DNA input requirement. It is an ideal approach for de novo sequencing, genome finishing, and the detection of structural variation — even in the most complex genomes such as those derived from cancer.

  • Sequence precious samples when limited DNA is available
  • Prepare sequencing-ready libraries in less than two days
  • Generate highly diverse libraries with premier data quality

Requires 10 times less DNA input than other protocols

Both gel-free and gel-plus protocols are available. The gel-free protocol allows mate pair sequencing when limited DNA is available:

  • Designed for applications such as de novo assembly of small genomes and detection of structural variation
  • The industry's only gel-free protocol with such low DNA input requirements

The gel-plus protocol is ideal for challenging mate pair applications, such as de novo assembly of complex genomes and detection of structural variation.

Fast and simple mate pair workflow

Inclusion of the TruSeq DNA Sample Preparation master-mixed reagents in the Nextera Mate Pair kit reduces the number of assay steps, thereby reducing the hands-on time required.

  • Reduction in pipetting steps simplifies workflow and reduces sample loss
  • Gel-free protocol option and on-bead reactions simplify purification steps and shorten protocol hands-on time

Increased library diversity and data quality

The Nextera Mate Pair Sample Preparation Kit provides identifiable junction sequences for accurate identification of the two halves of the mate pair fragment.

  • Only biotinylates DNA molecules at fragmentation sites, avoiding troublesome internal biotinylation
  • Allows for the creation of millions of unique fragments
  • Increased library diversity generates fewer duplicate reads and premier data quality

Featured Researcher


Diana Le Duc
“Nextera Mate Pair and the HiSeq System, make it so easy to assemble genomes and come to biological conclusions based on the data.”

Researcher at the Max Planck Institute for Evolutionary Anthropology

Read Interview »




System Compatibility

Genome Analyzer IIx

Catalog IDs: FC-132-1001