PhiX Control v3

PhiX Control v3 is a reliable, adapter-ligated library used as a control for Illumina sequencing runs. The library is derived from the small, well-characterized PhiX genome, offering several benefits for sequencing and alignment.

The PhiX library provides a quality control for cluster generation, sequencing, and alignment, and a calibration control for cross-talk matrix generation, phasing, and prephasing. It can be rapidly aligned to estimate relevant sequencing by synthesis (SBS) metrics such as phasing and error rate. Depending on the application, PhiX Control v3 may also be utilized:

 

  • As a high-concentration spike-in control for unbalanced samples (genomes with AT or GC content of less than 40% or greater than 60%)
  • As a low-concentration spike-in control for alignment calculations and quantification efficiency
  • As a dedicated control lane alongside low-diversity samples 
  • As a control for troubleshooting cluster generation problems, allowing you to quickly determine whether an error is related to library preparation

The versatile PhiX Control v3 is provided as a ready-to-use library, and can be utilized in diverse applications to add value to your workflow and increase confidence in your results.

System Compatibility

Genome Analyzer IIx

Product Literature

Description Document Type File Info
Low-Diversity Sequencing on the Illumina HiSeq Platforms Technical Note PDF(< 1 MB)
Data Analysis: Microarray Gene Expression Technical Note PDF(< 1 MB)
Illumina Genome Network Cancer Analysis Service Data Sheet PDF(< 1 MB)
DNA-Seq Data Processing Technical Note PDF(< 1 MB)
RNA-Seq Data Processing Technical Note PDF(< 1 MB)
Meta-Analysis Framework Technical Note PDF(< 1 MB)
Data Analysis: GWAS Processing Technical Note PDF(< 1 MB)
Using a PhiX Control for HiSeq Sequencing Runs Technical Note PDF(< 1 MB)
Data Correlation Details: Enrichment Analysis Technical Note PDF(< 1 MB)
TruSeq v3 Advancements for HiSeq Systems Brochure PDF(1 MB)
Accurate IGN Cancer Sequencing with a Combined-Calling Method Technical Note PDF(< 1 MB)
HiSeq Sequencing Systems Data Sheet PDF(< 1 MB)
Estimating Sequencing Coverage Technical Note PDF(< 1 MB)
Illumina Sequencing Portfolio Brochure,Sequencing Systems Portfolio Brochure PDF(9 MB)
De novo Bacterial Sequencing on the MiSeq System Technical Note PDF(< 1 MB)
Configuring Virus Scanner Software on Illumina Sequencers Technical Note PDF(< 1 MB)
A Patient-Centric SNV-CNV Pipeline Technical Note PDF(< 1 MB)

Catalog IDs: FC-110-3001