TruSeq DNA PCR-Free Library Preparation Kits provide simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to whole-human genomes. The kits offer shortened gel-free workflows, the ability to sequence the most challenging regions, and the power to identify the greatest number of variants. Kits include reagents, sample purification beads, and indexes, with two options for flexibility:
Sequence the most challenging regions
TruSeq DNA PCR-free kits offer superior coverage of areas which are traditionally difficult to sequence such as high GC-rich regions, promoters, and repetitive content. The kits are tunable to a variety of read lengths and are supported on all of Illumina's sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.
Detect the greatest number of variants
PCR-free means reduced library bias and gaps. The result is unsurpassed data quality. This enables you to detect the greatest number of variants. Excellent genome coverage means your results have the lowest and smallest number of gaps and enhanced coverage of high GC/AT rich regions.
Use PCR-free for faster protocols
The industry's most widely adopted library preparation workflow for DNA sequencing is now enhanced. Removing PCR creates a faster protocol and superior data quality. Bead-based size selection shortens the workflow. In tandem with Illumina's sequencing systems, the TruSeq DNA PCR-Free Library Preparation Kit provides a range of enhancements to the industry's most widely adopted library preparation workflow.
Kits for limited available DNA and exome enrichment
TruSeq Nano DNA Library Prep is also available for efficient interrogation of samples with limited available DNA.
For enrichment, the Nextera Rapid Capture Exome and Expanded Exome assays provide a seamless end-to-end library preparation solution. These all-in-one library preparation and exome enrichment kits allow researchers to identify coding variants 70% faster than any other method. An expanded kit increases coverage to UTRs and miRNA.
|Specification||TruSeq Nano DNA||TruSeq DNA PCR-Free|
|Kit type||For NeoPrep System||LT and HT||LT and HT|
|Description||TruSeq Nano DNA workflow performed with the NeoPrep system and reagents||Based upon widely adopted TruSeq library prep, with lower input and improved data quality||Superior genomic coverage with radically reduced library bias and gaps|
|Workflow||Simplified NeoPrep workflow with lower input and less hands-on time||Manual workflow||Manual workflow|
|Input quantity||25–75 ng||100–200 ng
|Assay time||7.5 hours (includes library prep through normalization)||5.5 hours||~5 hours|
|Hands-on time||30 minutes||4 hours||~4 hours|
|Target insert size||350 bp or 550 bp||350 bp or 550 bp||350 bp or 550 bp|
|Number of samples supported||Up to 16 samples per NeoPrep library card||24 (LT) or 96 (HT)||24 (LT) or 96 (HT) samples|
|Applications||Whole-genome sequencing applications, including whole-genome resequencing, de novo assembly, and metagenomics studies|
|Sample multiplexing||24 single indexes or 96 dual-index combinations|
|Compatible Illumina sequencers||MiniSeq, MiSeq, NextSeq, HiSeq, HiSeq X, HiScanSQ, and Genome Analyzer systems|
|Description||Document Type||File Info|
|TruSeq DNA PCR-Free||Data Sheet||PDF(8 MB)|
|Comparison of TruSeq Library Preparation Kits||Technical Note||PDF(< 1 MB)|
Catalog IDs: FC-121-3001, FC-121-3002, FC-121-3003