The TruSeq Rapid Exome Library Prep Kit delivers a simple, efficient method for high-confidence calling of exonic variants. The fast library preparation and exome enrichment workflow deliver libraries in 1 day with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. This kit offers:
|Enhanced transposome chemistry is highly tolerant of varying DNA input amounts, reducing bias and providing consistent results. Results were generated on a Bioanalyzer instrument.|
Illumina provides an integrated, fully supported workflow solution that guides researchers from library preparation through simple data analysis. Expert Illumina scientists provide a single source of technical and field support.
The TruSeq Rapid Exome Library Prep Kit delivers an average of 75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels, but still achieves uniform coverage for high-confidence results.
To learn more about calculating coverage estimates, see the sequencing coverage calculator.
|Sequencing System||No. of Exome Per Run at 50x||No. of Exome Per Run at 100x|
|NextSeq Mid-output flow cell||3||2|
|NextSeq High-output flow cell||12||6|
|HiSeq 2500 Rapid-Run (dual flow cell)||24||12|
|HiSeq 2500 High-output (dual flow cell)||156||78|
|HiSeq 4000 (dual flow cell)||192||96|
Estimated number of exomes sequenced per run is calculated with a mean coverage of 50x (4 Gb) and 100x (8 Gb), respectively. Illumina recommends a 2 × 75 bp read length on all sequencers when using the TruSeq Rapid Exome Library Prep Kit.
|Catalog Number||TruSeq Rapid Exome Library Prep Kit||Total Samples||Number of Enrichment Reactions||Number of Samples per Enrichment Reaction||Unique index adapter combinations|
|FC-144-1000||8 rxn x 1 plex||8||8||1||1|
|FC-144-1001||8 rxn x 3 plex||24||8||3||3|
|FC-144-1002||8 rxn x 6 plex||48||8||6||6|
|FC-144-1003||8 rxn x 9 plex||72||8||9||9|
|FC-144-1004||8 rxn x 12 plex||96||8||12||24|
|Description||Document Type||File Info|
Catalog IDs: FC-144-1000, FC-144-1001, FC-144-1002, FC-144-1003, FC-144-1004, FC-144-1005, FC-144-1006, FC-144-1007