TruSeq Targeted RNA Expression Kits

TruSeq Targeted RNA Expression Kits offer highly customizable mid- to high-plex gene expression profiling and validation for desktop sequencers. Overcoming significant challenges in cost and workflow, TruSeq Targeted RNA assays deliver a fully integrated solution, including convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.

User-defined panels of 12-1,000 assays can be created to target genes, isoforms, splice junctions, cSNPs, and fusion genes with the ability to multiplex up to 384 samples. Unleash the power of sequencing-based gene expression analysis, and generate data equivalent of up to 25,000 individual RT-PCR assays in a single run.*

TruSeq Targeted RNA Fixed Panels

For the ultimate in flexibility, custom content can be added to fully optimized, experimentally validated TruSeq Targeted RNA fixed panels focused on cell pathways and disease states.



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Start a new TruSeq Targeted RNA Expression project in DesignStudio, a web-based design tool for custom targeting projects. Start Now »


Genomics Demystifies the Gears of Circadian Clocks

Dr. Hughes at University of Missouri-St. Louis uses TruSeq Targeted RNA Expression to uncover the genetic mechanisms of circadian rhythms.
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TruSeq Targeted RNA Sample Datasets

Human Brain Reference RNA (HBRR), Universal Human Reference RNA (UHRR), and human liver RNA samples were prepared using TruSeq Targeted RNA Expression Kits using a combination of the cell cycle and NFKB panels. These libraries were sequenced on the MiniSeq System using the new MiniSeq High Output Reagent Kit at a 1 x 51 bp read length configuration. The total yield was 2.04 Gb with 96.5% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:



For Research Use Only.

*Refers to instruments with catalog number SY-410-1001 and upgrade, or catalog number SY-410-1003

Product Literature

Description Document Type File Info
Considerations for Designing a Successful TruSeq Targeted RNA Expression Experiment Technical Note PDF< 1 MB
TruSeq Targeted RNA Expression Data Sheet Data Sheet PDF< 1 MB
Expression Analysis of FFPE Samples Technical Note PDF1 MB

Catalog IDs: RT-101-1001, RT-102-1001, RT-801-1001, RT-802-1001, RT-401-1001, RT-402-1001, RT-402-1002, RT-402-1003, RT-402-1004