TruSeq Small RNA Library Preparation Kits

TruSeq Small RNA Library Preparation Kits provide a simple, cost-effective solution for generating small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters. These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing microRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species.

Learn more about small RNA sequencing.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

System Compatibility

Genome Analyzer IIx

Catalog IDs: RS-200-0012, RS-200-0024, RS-200-0036, RS-200-0048, 20005613, 20005614, 20005615, 20005616