TruSeq Stranded Total RNA Library Prep Kit

The TruSeq Stranded Total Library Preparation kit provides the clearest and most complete view of the transcriptome with a streamlined, cost-efficient and scalable solution for total RNA analysis with sequencing.

Whole-transcriptome analysis with precise measurement of strand orientation

Compatible with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Flexible solutions for a range of study designs

The TruSeq Stranded Total RNA Library Prep kits are available in multiple versions to fit specific study design needs:

  • TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.
  • TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.

All are designed against human, mouse and rat sequences but may be applicable to a variety of eukaryotic species.*

Kit configuration options

Each kit is available in low-throughput (LT) and high-throughput (HT) configurations (48 samples / 12 of 24 total indexes in Sets A and B and 96 samples, 96 unique dual-index pairs, respectively) and with protocols for the preparation of both small and large numbers of samples.

Related links

Find RNA-Seq solutions for:

Visit the RNA-Seq overview page for more information, protocols, and references.

*For information on a particular species of interest, please contact Illumina Technical Support.

Limited Use Label License: This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc.  The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity).  The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product.  The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.

System Compatibility

Genome Analyzer IIx

Product Literature

Description Document Type File Info
TruSeq Stranded mRNA and Total RNA Data Sheet PDF(8 MB)

Catalog IDs: RS-122-2201, RS-122-2202, RS-122-2203, RS-122-2301, RS-122-2302, RS-122-2303