Nextera Flex

快速、灵活的工作流程,提供省时的测序解决方案和优化的文库制备方案

Nextera Flex技术是Illumina文库制备系列产品中最快速、灵活的DNA测序解决方案。On-bead tagmentation测序化学技术减少了全基因组和靶向富集工作流程的总时间。

Nextera Flex产品代表了Illumina文库制备测序化学技术的最新进展。独特的连接微珠的转座子组技术现在集成了DNA提取、片段化、文库制备和文库归一化步骤,在Illumina文库制备系列产品中具有最快速、灵活的工作流程。

在新一代测序(NGS)工作流程中,文库制备时间太长可能会耗费大量成本。Illumina Nextera Flex文库制备技术为开发快速的工作流程铺平了道路,缩短了测序之前的延迟。

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Nextera Flex技术可用于减少从DNA提取到文库归一化的文库制备时间,其应用包括:

  • 用于任何物种(大型或小型)的全基因组测序
  • 对大量感兴趣的基因组区域进行测序,包括Illumina或第三方寡核苷酸供应商的全外显子组
  • 使用预先设计并固定基因集的panel对疾病或表型相关的靶点进行测序
  • 使用定制panel对感兴趣的区域或靶点进行测序

在满足不同研究需求的同时,Nextera Flex技术可以提供大小一致的插入片段、均一的覆盖度和优化的性能,不论DNA起始量或基因组大小如何。这种微珠技术兼容自动化,最大限度地减少了偏向性和出错几率,能生成高度可重现的测序数据。

Nextera Flex测序解决方案

org.apache.sling.api.scripting.ScriptEvaluationException: at org.apache.sling.scripting.core.impl.DefaultSlingScript.call(DefaultSlingScript.java:416) at org.apache.sling.scripting.core.impl.DefaultSlingScript.eval(DefaultSlingScript.java:184) at org.apache.sling.scripting.core.impl.DefaultSlingScript.service(DefaultSlingScript.java:491) at org.apache.sling.engine.impl.request.RequestData.service(RequestData.java:552) at org.apache.sling.engine.impl.filter.SlingComponentFilterChain.render(SlingComponentFilterChain.java:44) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:77) at com.day.cq.wcm.core.impl.WCMDebugFilter.doFilterWithErrorHandling(WCMDebugFilter.java:192) at com.day.cq.wcm.core.impl.WCMDebugFilter.doFilter(WCMDebugFilter.java:159) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.dynamicinclude.IncludeTagFilter.doFilter(IncludeTagFilter.java:71) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.WCMComponentFilter.doFilter(WCMComponentFilter.java:278) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.PageLockFilter.doFilter(PageLockFilter.java:91) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.personalization.impl.TargetComponentFilter.doFilter(TargetComponentFilter.java:96) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.processComponent(SlingRequestProcessorImpl.java:282) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.dispatchRequest(SlingRequestProcessorImpl.java:322) at org.apache.sling.engine.impl.request.SlingRequestDispatcher.dispatch(SlingRequestDispatcher.java:211) at org.apache.sling.engine.impl.request.SlingRequestDispatcher.include(SlingRequestDispatcher.java:104) at com.day.cq.wcm.core.impl.WCMComponentFilter$ForwardRequestDispatcher.include(WCMComponentFilter.java:516) at com.day.cq.wcm.tags.IncludeTag.includeResource(IncludeTag.java:254) at com.day.cq.wcm.tags.IncludeTag.doEndTag(IncludeTag.java:103) at org.apache.jsp.apps.illumina_002dmarketing.components.bootstrapColumnsWithRows.bootstrapColumnsWithRows_jsp._jspService(bootstrapColumnsWithRows_jsp.java:289) at org.apache.sling.scripting.jsp.jasper.runtime.HttpJspBase.service(HttpJspBase.java:70) at javax.servlet.http.HttpServlet.service(HttpServlet.java:725) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:502) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:449) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.callJsp(JspScriptEngineFactory.java:346) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.access$100(JspScriptEngineFactory.java:101) at org.apache.sling.scripting.jsp.JspScriptEngineFactory$JspScriptEngine.eval(JspScriptEngineFactory.java:607) at org.apache.sling.scripting.core.impl.DefaultSlingScript.call(DefaultSlingScript.java:388) at org.apache.sling.scripting.core.impl.DefaultSlingScript.eval(DefaultSlingScript.java:184) at org.apache.sling.scripting.core.impl.DefaultSlingScript.service(DefaultSlingScript.java:491) at org.apache.sling.engine.impl.request.RequestData.service(RequestData.java:552) at org.apache.sling.engine.impl.filter.SlingComponentFilterChain.render(SlingComponentFilterChain.java:44) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:77) at com.day.cq.wcm.core.impl.WCMDebugFilter.doFilterWithErrorHandling(WCMDebugFilter.java:192) at com.day.cq.wcm.core.impl.WCMDebugFilter.doFilter(WCMDebugFilter.java:159) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.dynamicinclude.IncludeTagFilter.doFilter(IncludeTagFilter.java:71) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.WCMComponentFilter.doFilter(WCMComponentFilter.java:278) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.PageLockFilter.doFilter(PageLockFilter.java:91) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.personalization.impl.TargetComponentFilter.doFilter(TargetComponentFilter.java:96) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.processComponent(SlingRequestProcessorImpl.java:282) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.dispatchRequest(SlingRequestProcessorImpl.java:322) at org.apache.sling.engine.impl.request.SlingRequestDispatcher.dispatch(SlingRequestDispatcher.java:211) at org.apache.sling.engine.impl.request.SlingRequestDispatcher.include(SlingRequestDispatcher.java:104) at com.day.cq.wcm.core.impl.WCMComponentFilter$ForwardRequestDispatcher.include(WCMComponentFilter.java:516) at org.apache.sling.scripting.jsp.taglib.IncludeTagHandler.dispatch(IncludeTagHandler.java:54) at org.apache.sling.scripting.jsp.taglib.AbstractDispatcherTagHandler.doEndTag(AbstractDispatcherTagHandler.java:129) at org.apache.jsp.libs.foundation.components.parsys.parsys_jsp._jspService(parsys_jsp.java:322) at org.apache.sling.scripting.jsp.jasper.runtime.HttpJspBase.service(HttpJspBase.java:70) at javax.servlet.http.HttpServlet.service(HttpServlet.java:725) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:502) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:449) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.callJsp(JspScriptEngineFactory.java:346) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.access$100(JspScriptEngineFactory.java:101) at org.apache.sling.scripting.jsp.JspScriptEngineFactory$JspScriptEngine.eval(JspScriptEngineFactory.java:607) at org.apache.sling.scripting.core.impl.DefaultSlingScript.call(DefaultSlingScript.java:388) at org.apache.sling.scripting.core.impl.DefaultSlingScript.eval(DefaultSlingScript.java:184) at org.apache.sling.scripting.core.impl.DefaultSlingScript.service(DefaultSlingScript.java:491) at org.apache.sling.engine.impl.request.RequestData.service(RequestData.java:552) at org.apache.sling.engine.impl.filter.SlingComponentFilterChain.render(SlingComponentFilterChain.java:44) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:77) at com.day.cq.wcm.core.impl.WCMDebugFilter.doFilterWithErrorHandling(WCMDebugFilter.java:192) at com.day.cq.wcm.core.impl.WCMDebugFilter.doFilter(WCMDebugFilter.java:159) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.dynamicinclude.IncludeTagFilter.doFilter(IncludeTagFilter.java:71) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.WCMComponentFilter.doFilter(WCMComponentFilter.java:278) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.PageLockFilter.doFilter(PageLockFilter.java:91) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.personalization.impl.TargetComponentFilter.doFilter(TargetComponentFilter.java:96) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.processComponent(SlingRequestProcessorImpl.java:282) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.dispatchRequest(SlingRequestProcessorImpl.java:322) at org.apache.sling.engine.impl.request.SlingRequestDispatcher.dispatch(SlingRequestDispatcher.java:211) at org.apache.sling.engine.impl.request.SlingRequestDispatcher.include(SlingRequestDispatcher.java:104) at com.day.cq.wcm.core.impl.WCMComponentFilter$ForwardRequestDispatcher.include(WCMComponentFilter.java:516) at com.day.cq.wcm.tags.IncludeTag.includeResource(IncludeTag.java:254) at com.day.cq.wcm.tags.IncludeTag.doEndTag(IncludeTag.java:103) at org.apache.jsp.apps.illumina_002dmarketing.components.page.bootstrapfullpage.body_jsp._jspx_meth_cq_005finclude_005f3(body_jsp.java:528) at org.apache.jsp.apps.illumina_002dmarketing.components.page.bootstrapfullpage.body_jsp._jspService(body_jsp.java:249) at org.apache.sling.scripting.jsp.jasper.runtime.HttpJspBase.service(HttpJspBase.java:70) at javax.servlet.http.HttpServlet.service(HttpServlet.java:725) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:502) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:449) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.callJsp(JspScriptEngineFactory.java:346) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.access$100(JspScriptEngineFactory.java:101) at org.apache.sling.scripting.jsp.JspScriptEngineFactory$JspScriptEngine.eval(JspScriptEngineFactory.java:607) at org.apache.sling.scripting.core.impl.DefaultSlingScript.call(DefaultSlingScript.java:388) at org.apache.sling.scripting.core.impl.DefaultSlingScript.eval(DefaultSlingScript.java:184) at org.apache.sling.scripting.core.impl.DefaultSlingScript.service(DefaultSlingScript.java:491) at com.day.cq.wcm.tags.IncludeTag.includeScript(IncludeTag.java:178) at com.day.cq.wcm.tags.IncludeTag.doEndTag(IncludeTag.java:95) at org.apache.jsp.apps.illumina_002dmarketing.components.page.bootstrapfullpage.bootstrapfullpage_jsp._jspx_meth_cq_005finclude_005f1(bootstrapfullpage_jsp.java:469) at org.apache.jsp.apps.illumina_002dmarketing.components.page.bootstrapfullpage.bootstrapfullpage_jsp._jspService(bootstrapfullpage_jsp.java:256) at org.apache.sling.scripting.jsp.jasper.runtime.HttpJspBase.service(HttpJspBase.java:70) at javax.servlet.http.HttpServlet.service(HttpServlet.java:725) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:502) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:449) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.callJsp(JspScriptEngineFactory.java:346) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.access$100(JspScriptEngineFactory.java:101) at org.apache.sling.scripting.jsp.JspScriptEngineFactory$JspScriptEngine.eval(JspScriptEngineFactory.java:607) at org.apache.sling.scripting.core.impl.DefaultSlingScript.call(DefaultSlingScript.java:388) at org.apache.sling.scripting.core.impl.DefaultSlingScript.eval(DefaultSlingScript.java:184) at org.apache.sling.scripting.core.impl.DefaultSlingScript.service(DefaultSlingScript.java:491) at org.apache.sling.engine.impl.request.RequestData.service(RequestData.java:552) at org.apache.sling.engine.impl.filter.SlingComponentFilterChain.render(SlingComponentFilterChain.java:44) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:77) at com.day.cq.wcm.core.impl.WCMDebugFilter.doFilterWithErrorHandling(WCMDebugFilter.java:192) at com.day.cq.wcm.core.impl.WCMDebugFilter.doFilter(WCMDebugFilter.java:159) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.dynamicinclude.IncludeTagFilter.doFilter(IncludeTagFilter.java:71) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.WCMComponentFilter.doFilter(WCMComponentFilter.java:278) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.PageLockFilter.doFilter(PageLockFilter.java:91) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.personalization.impl.TargetComponentFilter.doFilter(TargetComponentFilter.java:96) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.processComponent(SlingRequestProcessorImpl.java:282) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.dispatchRequest(SlingRequestProcessorImpl.java:322) at org.apache.sling.engine.impl.request.SlingRequestDispatcher.dispatch(SlingRequestDispatcher.java:211) at org.apache.sling.engine.impl.request.SlingRequestDispatcher.include(SlingRequestDispatcher.java:104) at com.day.cq.wcm.core.impl.WCMComponentFilter$ForwardRequestDispatcher.include(WCMComponentFilter.java:516) at org.apache.jsp.libs.cq.Page.Page_jsp._jspService(Page_jsp.java:117) at org.apache.sling.scripting.jsp.jasper.runtime.HttpJspBase.service(HttpJspBase.java:70) at javax.servlet.http.HttpServlet.service(HttpServlet.java:725) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:502) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:449) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.callJsp(JspScriptEngineFactory.java:346) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.access$100(JspScriptEngineFactory.java:101) at org.apache.sling.scripting.jsp.JspScriptEngineFactory$JspScriptEngine.eval(JspScriptEngineFactory.java:607) at org.apache.sling.scripting.core.impl.DefaultSlingScript.call(DefaultSlingScript.java:388) at org.apache.sling.scripting.core.impl.DefaultSlingScript.eval(DefaultSlingScript.java:184) at org.apache.sling.scripting.core.impl.DefaultSlingScript.service(DefaultSlingScript.java:491) at org.apache.sling.engine.impl.request.RequestData.service(RequestData.java:552) at org.apache.sling.engine.impl.filter.SlingComponentFilterChain.render(SlingComponentFilterChain.java:44) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:77) at com.day.cq.wcm.core.impl.WCMDebugFilter.doFilter(WCMDebugFilter.java:156) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.WCMComponentFilter.filterRootInclude(WCMComponentFilter.java:375) at com.day.cq.wcm.core.impl.WCMComponentFilter.doFilter(WCMComponentFilter.java:190) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.PageLockFilter.doFilter(PageLockFilter.java:91) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.personalization.impl.TargetComponentFilter.doFilter(TargetComponentFilter.java:96) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.processComponent(SlingRequestProcessorImpl.java:282) at org.apache.sling.engine.impl.filter.RequestSlingFilterChain.render(RequestSlingFilterChain.java:49) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:77) at org.apache.sling.dynamicinclude.SyntheticResourceFilter.doFilter(SyntheticResourceFilter.java:66) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:73) at com.day.cq.dam.core.impl.assetlinkshare.AdhocAssetShareAuthHandler.doFilter(AdhocAssetShareAuthHandler.java:437) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.dam.core.impl.servlet.ActivityRecordHandler.doFilter(ActivityRecordHandler.java:154) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.adobe.cq.social.ugcbase.security.impl.SaferSlingPostServlet.doFilter(SaferSlingPostServlet.java:114) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:73) at com.adobe.granite.rest.impl.servlet.ApiResourceFilter.doFilter(ApiResourceFilter.java:70) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.adobe.granite.requests.logging.impl.RequestLoggerImpl.doFilter(RequestLoggerImpl.java:126) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.adobe.granite.rest.assets.impl.AssetContentDispositionFilter.doFilter(AssetContentDispositionFilter.java:96) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.adobe.granite.csrf.impl.CSRFFilter.doFilter(CSRFFilter.java:217) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.security.impl.ContentDispositionFilter.doFilter(ContentDispositionFilter.java:180) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.AuthoringUIModeServiceImpl.doFilter(AuthoringUIModeServiceImpl.java:292) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.mobile.core.impl.redirect.RedirectFilter.doFilter(RedirectFilter.java:248) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.illumina.aem.impl.filters.LoggingFilter.doFilter(LoggingFilter.java:40) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.illumina.cookies.impl.filters.CookieManagerFilter.doFilter(CookieManagerFilter.java:97) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.illumina.cookies.impl.filters.LoggingFilter.doFilter(LoggingFilter.java:40) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.dynamicinclude.CacheControlFilter.doFilter(CacheControlFilter.java:67) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.engine.impl.debug.RequestProgressTrackerLogFilter.doFilter(RequestProgressTrackerLogFilter.java:107) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.adobe.cq.social.commons.cors.CORSAuthenticationFilter.doFilter(CORSAuthenticationFilter.java:91) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.foundation.forms.FormsHandlingServletHelper.handleFilter(FormsHandlingServletHelper.java:221) at com.day.cq.wcm.foundation.forms.impl.FormsHandlingServlet.doFilter(FormsHandlingServlet.java:138) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.adobe.granite.optout.impl.OptOutFilter.doFilter(OptOutFilter.java:76) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.foundation.forms.FormsHandlingServletHelper.handleFilter(FormsHandlingServletHelper.java:221) at com.adobe.cq.wcm.core.components.internal.servlets.CoreFormHandlingServlet.doFilter(CoreFormHandlingServlet.java:131) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.WCMRequestFilter.doFilter(WCMRequestFilter.java:90) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.adobe.cq.mcm.campaign.servlets.CampaignCopyTracker.doFilter(CampaignCopyTracker.java:100) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.rewriter.impl.RewriterFilter.doFilter(RewriterFilter.java:83) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.adobe.granite.httpcache.impl.InnerCacheFilter.doFilter(InnerCacheFilter.java:81) at com.adobe.granite.httpcache.impl.InnerCacheFilter.doFilter(InnerCacheFilter.java:60) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.i18n.impl.I18NFilter.doFilter(I18NFilter.java:131) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:73) at com.adobe.granite.resourceresolverhelper.impl.ResourceResolverHelperImpl.doFilter(ResourceResolverHelperImpl.java:83) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.doProcessRequest(SlingRequestProcessorImpl.java:151) at org.apache.sling.engine.impl.SlingMainServlet.service(SlingMainServlet.java:219) at org.apache.felix.http.base.internal.handler.ServletHandler.handle(ServletHandler.java:85) at org.apache.felix.http.base.internal.dispatch.InvocationChain.doFilter(InvocationChain.java:79) at com.adobe.granite.license.impl.LicenseCheckFilter.doFilter(LicenseCheckFilter.java:308) at org.apache.felix.http.base.internal.handler.FilterHandler.handle(FilterHandler.java:135) at org.apache.felix.http.base.internal.dispatch.InvocationChain.doFilter(InvocationChain.java:74) at org.apache.felix.http.sslfilter.internal.SslFilter.doFilter(SslFilter.java:96) at org.apache.felix.http.base.internal.handler.FilterHandler.handle(FilterHandler.java:135) at org.apache.felix.http.base.internal.dispatch.InvocationChain.doFilter(InvocationChain.java:74) at org.apache.sling.security.impl.ReferrerFilter.doFilter(ReferrerFilter.java:295) at org.apache.felix.http.base.internal.handler.FilterHandler.handle(FilterHandler.java:135) at org.apache.felix.http.base.internal.dispatch.InvocationChain.doFilter(InvocationChain.java:74) at org.apache.sling.i18n.impl.I18NFilter.doFilter(I18NFilter.java:131) at org.apache.felix.http.base.internal.handler.FilterHandler.handle(FilterHandler.java:135) at org.apache.felix.http.base.internal.dispatch.InvocationChain.doFilter(InvocationChain.java:74) at org.apache.sling.featureflags.impl.FeatureManager.doFilter(FeatureManager.java:116) at org.apache.felix.http.base.internal.handler.FilterHandler.handle(FilterHandler.java:135) at org.apache.felix.http.base.internal.dispatch.InvocationChain.doFilter(InvocationChain.java:74) at org.apache.sling.engine.impl.log.RequestLoggerFilter.doFilter(RequestLoggerFilter.java:72) at org.apache.felix.http.base.internal.handler.FilterHandler.handle(FilterHandler.java:135) at org.apache.felix.http.base.internal.dispatch.InvocationChain.doFilter(InvocationChain.java:74) at org.apache.felix.http.base.internal.dispatch.Dispatcher.dispatch(Dispatcher.java:127) at org.apache.felix.http.base.internal.dispatch.DispatcherServlet.service(DispatcherServlet.java:49) at javax.servlet.http.HttpServlet.service(HttpServlet.java:725) at org.eclipse.jetty.servlet.ServletHolder.handle(ServletHolder.java:812) at org.eclipse.jetty.servlet.ServletHandler.doHandle(ServletHandler.java:587) at org.eclipse.jetty.server.session.SessionHandler.doHandle(SessionHandler.java:221) at org.eclipse.jetty.server.handler.ContextHandler.doHandle(ContextHandler.java:1127) at org.eclipse.jetty.servlet.ServletHandler.doScope(ServletHandler.java:515) at org.eclipse.jetty.server.session.SessionHandler.doScope(SessionHandler.java:185) at org.eclipse.jetty.server.handler.ContextHandler.doScope(ContextHandler.java:1061) at org.eclipse.jetty.server.handler.ScopedHandler.handle(ScopedHandler.java:141) at org.eclipse.jetty.server.handler.ContextHandlerCollection.handle(ContextHandlerCollection.java:215) at org.eclipse.jetty.server.handler.HandlerWrapper.handle(HandlerWrapper.java:97) at org.eclipse.jetty.server.Server.handle(Server.java:499) at org.eclipse.jetty.server.HttpChannel.handle(HttpChannel.java:311) at org.eclipse.jetty.server.HttpConnection.onFillable(HttpConnection.java:258) at org.eclipse.jetty.io.AbstractConnection$2.run(AbstractConnection.java:544) at org.eclipse.jetty.util.thread.QueuedThreadPool.runJob(QueuedThreadPool.java:635) at org.eclipse.jetty.util.thread.QueuedThreadPool$3.run(QueuedThreadPool.java:555) at java.lang.Thread.run(Unknown Source) Caused by: org.apache.sling.api.SlingException: at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.handleJspExceptionInternal(JspServletWrapper.java:691) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.handleJspException(JspServletWrapper.java:608) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:533) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:449) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.callJsp(JspScriptEngineFactory.java:346) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.access$100(JspScriptEngineFactory.java:101) at org.apache.sling.scripting.jsp.JspScriptEngineFactory$JspScriptEngine.eval(JspScriptEngineFactory.java:607) at org.apache.sling.scripting.core.impl.DefaultSlingScript.call(DefaultSlingScript.java:388) ... 258 more Caused by: org.apache.sling.api.SlingException: javax.servlet.ServletException: org.apache.sling.commons.json.JSONException: JSONObject["ctmapdetail"] is not a JSONArray. at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.handleJspExceptionInternal(JspServletWrapper.java:691) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.handleJspException(JspServletWrapper.java:608) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:525) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:449) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.callJsp(JspScriptEngineFactory.java:346) at org.apache.sling.scripting.jsp.JspScriptEngineFactory.access$100(JspScriptEngineFactory.java:101) at org.apache.sling.scripting.jsp.JspScriptEngineFactory$JspScriptEngine.eval(JspScriptEngineFactory.java:607) at org.apache.sling.scripting.core.impl.DefaultSlingScript.call(DefaultSlingScript.java:388) at org.apache.sling.scripting.core.impl.DefaultSlingScript.eval(DefaultSlingScript.java:184) at org.apache.sling.scripting.core.impl.DefaultSlingScript.service(DefaultSlingScript.java:491) at org.apache.sling.engine.impl.request.RequestData.service(RequestData.java:552) at org.apache.sling.engine.impl.filter.SlingComponentFilterChain.render(SlingComponentFilterChain.java:44) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:77) at com.day.cq.wcm.core.impl.WCMDebugFilter.doFilterWithErrorHandling(WCMDebugFilter.java:192) at com.day.cq.wcm.core.impl.WCMDebugFilter.doFilter(WCMDebugFilter.java:159) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.dynamicinclude.IncludeTagFilter.doFilter(IncludeTagFilter.java:71) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.WCMComponentFilter.doFilter(WCMComponentFilter.java:278) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.wcm.core.impl.PageLockFilter.doFilter(PageLockFilter.java:91) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at com.day.cq.personalization.impl.TargetComponentFilter.doFilter(TargetComponentFilter.java:96) at org.apache.sling.engine.impl.filter.AbstractSlingFilterChain.doFilter(AbstractSlingFilterChain.java:68) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.processComponent(SlingRequestProcessorImpl.java:282) at org.apache.sling.engine.impl.SlingRequestProcessorImpl.dispatchRequest(SlingRequestProcessorImpl.java:322) at org.apache.sling.engine.impl.request.SlingRequestDispatcher.dispatch(SlingRequestDispatcher.java:211) at org.apache.sling.engine.impl.request.SlingRequestDispatcher.include(SlingRequestDispatcher.java:104) at com.day.cq.wcm.core.impl.WCMComponentFilter$ForwardRequestDispatcher.include(WCMComponentFilter.java:516) at org.apache.sling.scripting.jsp.taglib.IncludeTagHandler.dispatch(IncludeTagHandler.java:54) at org.apache.sling.scripting.jsp.taglib.AbstractDispatcherTagHandler.doEndTag(AbstractDispatcherTagHandler.java:129) at org.apache.jsp.libs.foundation.components.parsys.parsys_jsp._jspService(parsys_jsp.java:322) at org.apache.sling.scripting.jsp.jasper.runtime.HttpJspBase.service(HttpJspBase.java:70) at javax.servlet.http.HttpServlet.service(HttpServlet.java:725) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:502) ... 263 more Caused by: org.apache.sling.commons.json.JSONException: JSONObject["ctmapdetail"] is not a JSONArray. at org.apache.sling.commons.json.JSONObject.getJSONArray(JSONObject.java:440) at org.apache.jsp.apps.illumina_002dmarketing.components.systemsGroup.systemdetailWorkflow.systemdetailWorkflow_jsp._jspService(systemdetailWorkflow_jsp.java:374) at org.apache.sling.scripting.jsp.jasper.runtime.HttpJspBase.service(HttpJspBase.java:70) at javax.servlet.http.HttpServlet.service(HttpServlet.java:725) at org.apache.sling.scripting.jsp.jasper.servlet.JspServletWrapper.service(JspServletWrapper.java:502) ... 295 more

Nextera Flex技术使用了一种创新的微珠转座子组复合物,通过在单一反应步骤中片段化并添加接头标签序列来对基因组DNA进行片段化处理。起始DNA饱和后,基于微珠的转座子组复合物能使一定数量的DNA分子片段化。这种基于微珠的饱和技术可以灵活地用于较宽的DNA起始量范围,并提供一致的、均一的片段大小分布和归一化的文库。在酶法片段化过程之后,利用有限循环的PCR步骤将Nextera DNA flex特异性标签接头序列添加到DNA片段的两端,以便开展混合文库的双标签测序。然后进行基于微珠的纯化步骤,制备适用于任意Illumina测序平台的文库。

Nextera DNA Flex Library Prep Kit内有制备测序文库所需的所有试剂,以及用于文库纯化步骤的样本纯化磁珠。

Nextera Flex for Enrichment Kit包含文库制备和富集所需的试剂。用于文库纯化步骤的AMPure XP微珠、标签接头以及您选择的富集寡核苷酸panel须单独订购。

以下panel与Nextera Flex for Enrichment兼容:

  • TruSight One
  • TruSight One Expanded
  • TruSight Cancer
  • TruSight Cardio
  • TruSeq Neurodegeneration

以下标签接头与Nextera DNA Flex兼容:

  • IDT for Illumina Nextera Unique Dual (UD) Indexes (10 bp)
  • Nextera Combinatorial Dual (CD) Indexes (8 bp)

以下标签接头与Nextera Flex for Enrichment兼容:

  • IDT for Illumina Nextera Unique Dual (UD) Indexes (10 bp)
目标富集

利用目标富集技术,只需单次分析即可对关键基因和感兴趣的区域进行测序。

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癌症基因组学

我们的癌症基因组学解决方案有助于获得癌症生物学的新见解。

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靶向重测序

通过靶向重测序,可以分离出一部分基因或基因组区域并进行测序,从而节约实验室资源。

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