Illumina DNA PCR-Free Prep is now available. This library prep kit achieves commensurate performance with TruSeq DNA PCR-Free, while reducing turnaround time and hands-on time.

TruSeq DNA PCR-Free

Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.Read More...
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Library Prep

TruSeq DNA PCR-Free Low Throughput Library Prep Kit (24 samples)

20015962

TruSeq DNA PCR-Free High Throughput Library Prep Kit (96 samples)

20015963


Index Adapters

TruSeq DNA Single Indexes Set A (12 Indexes, 24 Samples)

20015960

TruSeq DNA Single Indexes Set B (12 Indexes, 24 Samples)

20015961

TruSeq DNA CD Indexes (96 Indexes, 96 Samples)

20015949

IDT for Illumina – TruSeq DNA UD Indexes (24 Indexes, 96 Samples)

20020590

IDT for Illumina – TruSeq DNA UD Indexes (96 Indexes, 96 Samples)

20022370

配件产品

Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

Product Highlights

TruSeq DNA PCR-Free provides simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole genomes. The workflows offer:

  • Shortened gel-free workflows that remove the need for PCR
  • Ability to sequence challenging regions
  • Excellent coverage quality for deep insight into the genome
Sequence challenging regions

TruSeq DNA PCR-Free offers superior coverage of areas that are traditionally difficult to sequence, such as GC-rich regions, promoters, and repetitive content.

The workflows are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Gain deep insight into the genome

PCR-free means reduced library bias and gaps. The result is high data quality and optimal variant detection across the genome. Excellent genome coverage quality means your results have few gaps and good coverage of GC-rich regions.

Save time with a PCR-free protocol

Removing PCR saves time and removes genomic coverage bias associated with PCR steps. Bead-based size selection shortens the workflow. In tandem with Illumina sequencing systems, TruSeq DNA PCR-Free provides a range of enhancements to a widely adopted library preparation workflow.

Access flexible throughput options
  • TruSeq DNA PCR-Free with Single Indexes supports 24-sample manual processing for low-throughput (LT) studies.
  • TruSeq DNA PCR-Free with 96 CD Indexes supports 96-sample processing for high-throughput (HT) studies, and can be automated on liquid-handling robots (or processed manually).
  • IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell.

Find a list of robotic systems that support the HT workflows

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System 1 sample per run (based on 30× coverage of a human genome)  350 bp insert: ≤ 2 × 100 bp
550 bp insert: ≤ 2 × 150 bp
HiSeq 2500 System 2–10 samples per run (dual flow cell; based on 30× coverage of a human genome) 350 bp insert: ≤ 2 × 100 bp (rapid run)
550 bp insert: ≤ 2 × 250 bp (rapid run)
350 bp insert: ≤ 2 × 100 bp (high output)
550 bp insert: ≤ 2 × 125 bp (high output)
NovaSeq 6000 System Samples per run (dual flow cell): S1: 8, S2: 16, S4: 48 (based on 30× coverage of a human genome)  350 bp insert: ≤ 2 × 150 bp
550 bp insert: ≤ 2 × 150 bp

产品比较

TruSeq DNA PCR-Free Illumina DNA PCR-Free Prep Illumina DNA Prep TruSeq DNA Nano
Assay Time 5 hours total assay time ~1.5 hours ~3-4 hours (from DNA extraction to normalized library) ~6 hours total assay time
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Hands-On Time 4 hours ~45 minutes 1-1.5 hours ~4 hours
Input Quantity 1 ug DNA 25 ng to 300 ng Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide). 100 ng genomic DNA
Mechanism of Action Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. Bead-linked transposome Bead-linked transposome Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free.
Multiplexing Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) Up to 384-plex Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon)
Specialized Sample Types Not FFPE-Compatible Low-Input Samples Low-Input Samples, Not FFPE-Compatible
Species Details Compatible with most large DNA genomes. Compatible with most large DNA genomes.
Target Insert Size 350 bp or 550 bp 450 bp +/- 75 bp 350 bp or 550 bp

Method-Specific Workflow Example

 

Customer Stories

社交媒体明猫星接受测序

马普研究院和宾夕法尼亚大学的研究人员共同资助了Lil Bub的基因组测序,采用NextSeq 500系统揭示了此种猫罕见疾病的遗传因素。

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