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TruSight Tumor 15 uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 15 genes that are commonly mutated in solid tumors. It accurately detects low-frequency variants from 20 ng of starting DNA and is optimized for formalin-fixed, paraffin-embedded (FFPE) tumor tissue.
Featuring a rapid workflow that can be easily integrated into lab procedures, this panel offers a single assay for accurate, economical, and rapid analysis of solid tumors.
This TruSight Tumor panel offers:
3 human reference samples and 5 FFPE-exacted DNA samples from lung, colon, melanoma, and breast tumors were prepared using TruSight Tumor 15. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 150 bp read length configuration with dual indexing. The total yield was 7.6 Gb with 94.9% of bases at or above Q30.
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Instrument | Recommended Number of Samples | Read Length |
---|---|---|
MiniSeq System | Samples per run: mid output: 2-3, high output: 8 (based on 93.5% of the bases covered at 500x minimum) | 2 × 150 bp (max recommended) |
MiSeq System | 8 samples per run with v3 reagents (based on 93.5% of the bases covered at 500x minimum) | 2 × 151 bp (max recommended) |
AKT1 | GNA11 | NRAS |
BRAF | GNAQ | PDGFRA |
EGFR | KIT | PIK3CA |
ERBB2 | KRAS | RET |
FOXL2 | MET | TP53 |
Analysis of Cell-Free DNA with the TruSight Tumor 15 Assay
White paper | PDF < 1 MB
Assessing the Quality of FFPE Samples for Preparation of TruSight® Tumor 15 Sequencing Libraries
White paper | PDF < 1 MB
Analysis of TP53 Variants in Bone Marrow Aspirates With TruSight Tumor 15
White paper | PDF < 1 MB
Data sheet | PDF < 1 MB
Technical note | PDF < 1 MB
TruSight Tumor 15 Data Analysis
Technical note | PDF < 1 MB
A dedicated support section is not currently available for this product
Kit requires MiSeq Software v2.6 or higher and the Illumina Annotation Engine. Download files