Pillar® oncoReveal™ Myeloid Panel

A 58-gene pan-cancer panel with combined DNA content, utilizing the single-tube SLIMamp™ workflow for fast library prep and turnaround time.

~6.5 hr

Assay time

~3 hr

Hands-on time

10ng - 80ng DNA (20ng recommended)

Input quantity

See full details in the specifications table

Overview

The oncoReveal™ Myeloid Panel is a DNA NGS sequencing panel using proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

  • Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab

  • Achieve variant detection as low as 1% VAF without UIDs, even with limited DNA input or poor sample quality

  • Improve lab efficiency and reduce “no calls”, repeat testing and difficult interpretation decisions

  • < 8 hours DNA to sequencer


Specifications


Required Products

Pillar® oncoReveal™ panels require the use of a Pillar index kit. Pick your preferred number of reactions.

/ Results

Applications

Example workflow

1
Prep

Pillar® oncoReveal™ Myeloid Panel

Documentation

Product literature

Literature is not currently available for this product.

Support documentation

A dedicated support section is not currently available for this product

Compare

Pillar® oncoReveal™ Myeloid Panel AmpliSeq for Illumina Myeloid Panel TruSight Myeloid Sequencing Panel
Assay time ~6.5 hr
Cancer type Pan-cancer, Solid tumor
Description 58-gene amplicon based DNA panel, simple single tube workflow
Hands-on time ~3 hr
Input quantity 10ng - 80ng DNA (20ng recommended)
Instruments MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode
Mechanism of action Multiplex PCR using tiled amplicon
Method Targeted DNA sequencing, Amplicon sequencing
Nucleic acid type DNA
Number of amplicons 1 pool, 766 amplicons
Number of reactions 24 reactions
On-target reads 99.6% ± 0.2%
Specialized sample types Blood, Low-input samples
Species category Human
Target insert size Average: 215 bp
Technology Sequencing
Uniformity 96.8% ±1.0%
Variant class Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs)

Figures

Gene list

Panel specifications

oncoReveal™ Myeloid Panel

HDA-MY-1001-24

oncoReveal™ Myeloid Panel

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Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

IDX-PI-1001-96

Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

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Kit D, indices PI501-8, PI701-12

IDX-PI-1004-192

Kit D, indices PI501-8, PI701-12

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