AmpliSeq for Illumina BRCA Panel

Targeted research panel investigating somatic and germline variants in BRCA1 and BRCA2. Read More...
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Panel

AmpliSeq™ BRCA Panel for Illumina®

20019168

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Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

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AmpliSeq™ CD Indexes Set A for Illumina®

20019105

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AmpliSeq™ CD Indexes Set B for Illumina®

20019106

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AmpliSeq™ CD Indexes Set C for Illumina®

20019107

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AmpliSeq™ CD Indexes Set D for Illumina®

20019167

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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

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Accessory Products

AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

Price
 
 

AmpliSeq™ Library Equalizer for Illumina®

20019171

Price
 
 

Product Highlights

The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay for research of somatic and germline variants across BRCA1 and BRCA2 genes. Key features include:

Relevant Gene Content
  • Target all exonic regions and flanking intronic sequences of BRCA1 and BRCA2
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate Data
  • Detect somatic mutations as low as 5% variant allele frequency using local or cloud based analysis

The BRCA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

BRCA1 and BRCA2 are human tumor suppressor genes that, when carrying specific mutations, have been implicated in an increased risk for breast and ovarian cancers. This ready-to-use panel saves researchers the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

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Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
iSeq 100 System 12 samples per run (assumes minimum coverage of 500×)
96 samples per run (assumes minimum coverage of 50x)
2 × 150 bp
2 × 150 bp
MiniSeq System Samples per run: mid output: 24, high output: 80 (assumes minimum coverage of 500×) 2 × 150 bp
MiSeq System Samples per run at 500x minimum coverage (by reagent kit version): v2 nano: 3, v2 micro: 12, v2: 48, v3: 80
Samples per run at 50x minimum coverage (by reagent kit version): v2 nano: 32, v2 micro: 96
2 × 150 bp
2 × 150 bp

Product Comparison

AmpliSeq for Illumina BRCA Panel TruSight Cancer

Manuals and Support Information

Custom Protocol Selector
Generates customized, end-to-end instructions

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