Illumina Total RNA Prep with Ribo-Zero Plus provides exceptional performance for the analysis of coding and multiple forms of noncoding RNA. Gain high-confidence discovery of alternative transcripts, gene fusions, and allele-specific expression.
Illumina Stranded Total RNA Prep with Ribo-Zero Plus Microbiome effectively depletes unwanted host and bacterial rRNA from thousands of bacterial strains commonly found in the gut for high-quality metatranscriptomics applications.
Read the data sheet to learn more about metatranscriptomics applications.
Illumina Total RNA Prep with Ribo-Zero Plus is based on TruSeq ligation technology, which has been cited in over 9,926 publications since 2011. This ligation method results in high coverage uniformity, precise strand information, and robust and reliable library preparation even from degraded samples.
Learn more about ligation technology
Flexibility for Many Study Designs
Illumina Total RNA Prep with Ribo-Zero Plus supports a broad range of RNA inputs, from 1 ng to 1000 ng1. It’s compatible with various sample types, including formalin-fixed paraffin-embedded (FFPE) and other low-quality samples. The included Ribo-Zero Plus removes abundant RNA from multiple species, including human, mouse, rat, bacteria, and epidemiology samples.
Find compatible species
Illumina Stranded Total RNA Prep with Ribo-Zero Plus Microbiome provides robust depletion of abundant rRNA in complex microbial samples, including stool samples, for metatranscriptomic studies. This kit accommodates a broad range of RNA inputs from 25 ng to 1000 ng2, and depletes rRNA from human, mouse, rat, and thousands of bacterial species.
Fast, Automation-friendly Workflow
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.
Explore library prep automation solutions
Multiplex up to 384 samples and sequence in a single run. The number of samples sequenced per run depends on the throughput capacity of the sequencer.
1This input range is applicable to high-quality RNA. We recommend 10 ng input for best results and for FFPE or degraded samples.
2Minimum recommended input for Ribo-Zero Plus Microbiome is 25 ng.