Illumina DNA Prep and Illumina DNA PCR-Free Prep are available. These library prep solutions offer improved performance while reducing turnaround time and hands-on time compared to TruSeq DNA Nano.

TruSeq DNA Nano

Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA. Read More...
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Library Prep

TruSeq Nano DNA Low Throughput Library Prep Kit (24 samples)

20015964

Price
 
 

TruSeq Nano DNA High Throughput Library Prep Kit (96 samples)

20015965

Price
 
 

Index Adapters

TruSeq DNA Single Indexes Set A (12 Indexes, 24 Samples)

20015960

Price
 
 

TruSeq DNA Single Indexes Set B (12 Indexes, 24 Samples)

20015961

Price
 
 

TruSeq DNA CD Indexes (96 Indexes, 96 Samples)

20015949

Price
 
 

IDT for Illumina – TruSeq DNA UD Indexes (24 Indexes, 96 Samples)

20020590

Price
 
 

IDT for Illumina – TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples)

20040870

Price
 
 

IDT for Illumina – TruSeq DNA UD Indexes (96 Indexes, 96 Samples)

20022370

Price
 
 

Illumina Advantage Products

TG TruSeq® Nano DNA LT Sample Prep Kit, Set A (24 Samples)

TG-202-1001

Price
 
 

TG TruSeq® Nano DNA LT Sample Prep Kit, Set B (24 Samples)

TG-202-1002

Price
 
 

TG Truseq® Nano DNA Accessory Kit

TG-280-1001

Price
 
 
Accessory Products

Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

Price
 
 

Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

Price
 
 

Product Highlights

TruSeq DNA Nano enables efficient interrogation of samples with limited available DNA. Based on mechanical fragmentation–ligation chemistry, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

  • Designed for low sample input
  • High coverage quality
  • Accelerated library prep
Manual preparation of high-quality libraries in less than a day

TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

Use with limited DNA samples

TruSeq DNA Nano offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps

TruSeq DNA Nano reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. Truseq DNA Nano products are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Access flexible throughput options
  • TruSeq DNA Nano with Single Indexes supports 24-sample manual processing for low-throughput (LT) studies.
  • TruSeq DNA Nano with 96 CD Indexes  supports 96-sample processing for high-throughput (HT) studies, and can be automated on liquid-handling robots (or processed manually).
  • IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell.

Find automation vendors that support the 96-sample workflow

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 1 sample per run (based on 30× coverage of a human genome) 350 bp insert: ≤ 2 × 100 bp
550 bp insert: ≤ 2 × 150 bp
HiSeq 2500 System 2–10 samples per run (dual flow cell; based on 30× coverage of a human genome)  Rapid run, 350 bp insert: ≤ 2 × 100 bp
Rapid run, 550 bp insert: ≤ 2 × 250 bp
High output, 350 bp insert: ≤ 2 × 100 bp
High output, 550 bp insert: ≤ 2 × 125 bp

Product Comparison

Method-Specific Workflow Example

 

Customer Stories

Product Literature

TruSeq DNA Nano

Data Sheet | PDF 11 MB

Comparison of TruSeq Sample Preparation Kits

Technical Note | PDF < 1 MB

Manuals and Support Information

Custom Protocol Selector
Generates customized, end-to-end instructions

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