Focused investigation of key genes

Sequencing targeted genomic regions of interest reduces sequencing costs, time, and data analysis

Targeted Resequencing

With targeted resequencing, a subset of genes or regions of the genome are isolated and sequenced. Targeted approaches using next-generation sequencing (NGS) allow researchers to focus time, expenses, and data analysis on specific areas of interest. Such targeted analysis can include the exome (the protein-coding portion of the genome), specific genes of interest (custom content), targets within genes, or mitochondrial DNA.

Benefits of Targeted Resequencing

Targeted resequencing is simpler and more accessible than ever before. Find out how to access the focused power of targeted NGS.

Download Guide
  • Focuses on regions of interest, generating a smaller, more manageable data set
  • Reduces sequencing costs and data analysis burdens
  • Reduces turnaround time compared to broader approaches
  • Enables deep sequencing at high coverage levels for rare variant identification

Compared to broader approaches, such as whole-genome sequencing, targeted sequencing is a more cost-effective method for investigating areas of interest. Targeted resequencing enables researchers to focus on regions that are most likely to be involved in the phenotype under study, conserving resources and generating a smaller, more manageable data set. Targeted approaches can also deliver much higher coverage levels, allowing identification of variants that are rare and more expensive with whole-genome or Sanger sequencing.

SBS Technology
Using qPCR or Sanger sequencing today?

Find out how Illumina targeted sequencing can help you discover more.

View targeted NGS infographic 

Learn more about NGS vs. qPCR 

Compare NGS & Sanger sequencing 

Pipetting Image
How can I use NGS to analyze specific genes or DNA regions?

Explore common targeted NGS methods, from exome to amplicon sequencing and more.

Show key targeted NGS methods

Cancer Cells
How can I apply targeted NGS?

Discover, validate, or screen genetic variants with targeted sequencing for various research areas, from cancer to microbiology and more.

Explore uses of targeted NGS 
Identify and Track Infectious Disease Threats

NGS supports effective genomic surveillance strategies to reduce infectious disease transmission. Targeted sequencing can be used to monitor zoonotic or respiratory pathogens.

Learn More About Genomic Surveillance
Scientist in biohazard lab
Interested in receiving newsletters, case studies, and information on genomic analysis techniques? Enter your email address.
Targeted Sequencing for HIV Studies
Targeted Sequencing for HIV Studies

Researchers used targeted resequencing to detect minor variants associated with human immunodeficiency virus (HIV) tropism.

Read Interview
Transitioning to AmpliSeq for Illumina
Transitioning to AmpliSeq for Illumina

Our detailed guide is designed to help you transfer projects from TruSeq Custom Amplicon to AmpliSeq for Illumina panels.

View Guide
AmpliSeq for Illumina on the iSeq 100 System
AmpliSeq for Illumina on the iSeq 100 System

Find step-by-step guidance designed to help you transition your workflows to AmpliSeq for Illumina panels on the iSeq 100 System.

View Guide