Illumina now offers modular product ordering to enable flexibility in your workflows. You may continue to order our complete workflow by choosing one of the pre-assembled kits from the "Pre-Assembled Kits" tab.
Alternatively, to perform the Illumina-IDT protocol, select products from the categories shown in the "Choose Your Components" Tab. Visit the IDT xGen Exome Research Panel page to learn more about the panel or purchase the additional components required to complete the workflow.
The Illumina Free Adapter Blocking Reagent is an optional reagent that can be used to treat most types of sequencing libraries to reduce index hopping levels. The fast and simple protocol allows for treatment of one or a pool of libraries just prior to sequencing on any Illumina platform.
The Nextera Exome Kit, previously known as the TruSeq Rapid Exome Library Prep Kit, delivers a simple, efficient method for high-confidence calling of exonic variants. This fast library preparation and exome enrichment workflow delivers libraries in less than 2 days with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. Benefits include:
Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment
Rapid, automation-friendly workflow completes in less than 2 days with only 3 hours of hands-on time
Proven Nextera Data Quality
The Nextera Exome Kit delivers an average of >75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels but still achieves uniform coverage for high-confidence results.
Illumina has partnered with IDT to co-market a combined exome enrichment solution using the xGen® Exome Research Panel, which consists of 429,826 individually synthesized and quality-controlled xGen Lockdown® Probes. The panel spans a 39 Mb target region (19,396 genes) of the human genome, and covers 51 Mb of end-to-end tiled space. xGen Blocking Oligos (Cat. No.1075474, 1075475, or 1075476 for TruSeq libraries; contact IDT for Nextera libraries) and xGen Lockdown Reagents (Cat. No. 1072280 or 1072281) are also required.
Nextera DNA Exome (formerly known as TruSeq Rapid Exome) is the recommended replacement product for the Nextera Rapid Capture Exome and Expanded Exome Kits, which have been discontinued. Illumina remains committed to providing you with high-quality support and service.