Customers must consider all facets of laboratory processes as they prepare for large-scale sequencing operations. Illumina offers an integrated solution for implementing human whole-genome sequencing (WGS) at scale on the HiSeq X Series. This solution, Illumina SeqLab, is designed to provide the necessary tools for maximum laboratory efficiencies and improved scalability.
Illumina SeqLab is a combination of both products and services. It provides an end-to-end solution using:
Illumina has partnered with GenoLogics and Hamilton to offer preconfigured automation workflows and a first-class LIMS optimized to support HiSeq X Series laboratories.
The Illumina SeqLab workflow provides seamless integration from sample accessioning to data analysis using Illumina-validated methods and software: Illumina Automated Workflow Manager for the Hamilton Microlab STAR liquid-handling automation workstations, and Illumina Automated Bridge Software for the BaseSpace Clarity LIMS X Edition. This solution provides confidence in WGS results with positive sample tracking throughout the workflow and streamlined processes for maximum efficiencies.
This automation is essential for laboratories scaling to 18,000 genomes per year. It reduces cost per sample by minimizing human production errors, and improves turnaround times with streamlined processing—all while ensuring confidence in concordance between the samples received and the data generated.
As sequencing throughput continues to increase, it becomes critical to prevent data analysis bottlenecks in the sequencing workflow. Commercial analysis tools must also provide high-quality data and increasingly sophisticated algorithms to have a significant impact on disease-related research and health care.
HiSeq Analysis Software v2.0 offers a fast, accurate solution for high-throughput WGS data analysis. With pipelines for WGS analysis and tumor-normal somatic variant analysis, the software provides a full range of variant types. These variant types include single nucleotide variants (SNVs), insertion-deletions (indels), structural variants (SVs), and copy number variants (CNVs) from BCL files.
HiSeq Analysis Software v2.0 provides WGS data up to six times faster than other pipelines, without the large IT investment required. The software runs on commodity hardware, enabling customers to quickly scale their computing infrastructure to support HiSeq X Series operations.
Unlike homebrew solutions that piece-meal systems and services together, Illumina SeqLab provides a single turnkey solution. With Illumina SeqLab, customers can establish maximum efficiencies, achieve high-quality data standards, and accelerate toward their business objectives and research goals.