Read Length | 2 × 150 bp |
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Output | ≥ 90 Gb |
Total Time* | ≤ 35 hours |
Throughput | 8–96 samples/run depending on assay |
* Includes cluster generation, 2 × 150 bp dual-indexed sequencing, reagent purge, and post-run wash.
High-Output | ≥ 600 M paired-end reads |
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Bases with ≥ Q30 | > 75% |
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Total Overall Accuracy – Somatic Variant Caller | ≥ 99.98%† |
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Total Overall Accuracy – Germline Variant Caller | ≥ 99.99%† |
† Using a representative assay designed to query a variety of genes covering approximately 13,000 bases across 23 different chromosomes, and containing wide GC content (18% to 87%), indels (up to 25 base pairs), and homopolymers (7 to 13 nucleotides).
Power Requirements | 100–240 Volts AC @ 50/60 Hz, 600w max |
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RFID Radio Frequency | 13.56 MHz |
RFID Power | 200 mW |
LED | Green 510-525 nm, Red 645-655 nm; Laser diode: 780 nm, Class IIIb |
Laser | Class 1 laser product embedded with a Class 3B diode |
Temperature | Transportation and Storage: -10°C to 50°C. Operating Conditions: Maintain a lab temperature of 19°C to 25°C (22°C±3°C). |
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RFID Radio Frequency | 13.56 MHz |
W×D×H | 54 cm × 69 cm × 58 cm |
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Weight | (84.4 kg) 186 lbs |
Crated Weight | (163 kg) 360 lbs |
Base Unit | Dual Intel Xeon E5-2648L v3 |
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CPU | 1.8 GHz |
Memory | 128 GB |
Hard Drive | 2 × 2 TB |
Operating System | Windows 10 |
Specifications shown here are for IVD use. View research mode only specs, applicable only when running the NextSeq 550Dx instrument in in Research mode.
Learn more about the workflow and flexibility of the NextSeq 550Dx; perform both IVD testing and clinical research on the same instrument.
View Specification SheetThe NextSeq 550Dx System harnesses proven Illumina sequencing by synthesis (SBS) technology to deliver highly accurate data and robust performance for multiple applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
View SBS videoThe NextSeq 550Dx System leverages 2-channel SBS chemistry to offer efficient sequencing and data generation. 2-channel SBS reduces cycle and data processing times, while delivering the same high quality and accuracy that sets Illumina systems apart.
Read technology spotlightUnited States:
The NextSeq 550Dx instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue, when used for in vitro diagnostic (IVD) assays performed on the instrument. The NextSeq 550Dx instrument is not intended for whole genome or de novo sequencing. The NextSeq 550Dx instrument is to be used with registered and listed, cleared or approved, IVD reagents and analytical software.
European Union:
The NextSeq 550Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic (IVD) assays performed on the instrument. The NextSeq 550Dx instrument is to be used with specific registered, certified or approved IVD reagents and analytical software.