AmpliSeq for Illumina Exome Panel

Targeted research panel for sequencing the protein-coding regions of the human genome.Read More...
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Panel

AmpliSeq™ Exome Panel for Illumina®

20019166


Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103


Index Adapters

Ampliseq™ CD Indexes Large Volume for Illumina® (96 Indexes, 96 Samples)

20019108

配件产品

AmpliSeq™ for Illumina® Sample ID Panel

20019162

AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

AmpliSeq™ Library Equalizer for Illumina®

20019171

产品特色

The AmpliSeq for Illumina Exome Panel brings the speed and simplicity of PCR to exome sequencing, enabling researchers to sequence eight exomes in a single run and identify germline variants in less time. Key features include:

Fast exome library preparation
  • Prepare 8 exome libraries in < 7.5 hours with < 1.5 hours hands-on time
Comprehensive coverage
  • Access uniform and specific coverage; includes >97% of the Consensus Coding Sequences (CCDS) annotation
Accurate Data
  • Detect common single nucleotide variants (SNVs), copy number variants (CNVs), and small indels

The Exome Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System Samples per run: high output: 8 samples per run (based on >95% covered at 30×) 2 x 151 bp

产品比较

AmpliSeq for Illumina Exome Panel TruSeq DNA Exome Nextera DNA Exome Illumina DNA Prep with Enrichment
Assay Time < 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 2.5 days
Content Specifications > 97% of CCDS Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Description Enables researchers to prepare 8 exome libraries in <7.5 hours with <1.5 hours hands-on time. A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content.
Hands-On Time < 1.5 hours 6 hours
Input Quantity 50 ng minimum 100 ng genomic DNA
Mechanism of Action Multiplex PCR Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes
Method Amplicon Sequencing , Exome Sequencing Exome Sequencing Exome Sequencing Custom Sequencing, Exome Sequencing , 靶向DNA测序 , 靶向富集
Multiplexing 96 dual index combinations Up to 12-plex enrichment
On-Target Reads >80% >80%
Specialized Sample Types 不兼容FFPE FFPE Tissue Low-Input Samples Blood, FFPE Tissue, Saliva
Species Category 人类 人类 人类 Other, 人类
Uniformity >90% (% coverage at 20x for 4 Gb) >85% (% coverage at 20x for 4 Gb)

Method-Specific Workflow Example

 

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