TruSeq DNA Exome
此试剂盒提供经济实惠的外显子组测序解决方案，在各种read深度下都能提供卓越的靶点覆盖度。Read More...
TruSeq DNA Exome modular component options are discontinued. Alternative options are pre-assembled kits or Illumina DNA Prep with Enrichment with enhanced technology.
TruSeq Exome Kit（原名为TruSeq Exome Library Prep Kit）是一种经济实惠的文库制备和外显子组富集解决方案，可提供：
TruSeq Exome Kit支持12重预富集文库混合，每个流动槽通道可对多达12个文库进行测序，可最大限度地提高测序通量并进行变异鉴定。每次运行中测序的外显子组更多，让研究人员能最大限度地利用经费。
了解有关计算覆盖度估计值的更多信息，请参阅测序覆盖度计算器。
TruSeq Exome Kit可获得匹配率≥80%的测序read，实现高效、经济的外显子组测序。通过将TruSeq Exome Kit与使用边合成边测序（SBS）技术测序的Illumina系统结合起来，研究人员能鉴别出真正的编码变异，并最大限度地减少假阳性和假阴性结果。
Illumina提供了集成且受支持的工作流程解决方案，指导研究人员完成从文库制备到分析的整个过程。外显子组测序工作流程的所有部分作为整体进行设计、优化和分析验证。专业的Illumina科学家将为流程的每一环节提供技术和现场支持。
Illumina现提供模块化产品的预订，让您的工作流程变得灵活。
Illumina现已与IDT合作，共同销售采用xGen® Exome Research Panel v1.0（由429,826个单独合成且质量受控的xGen Lockdown® Probe组成）的联合外显子组富集解决方案。本panel涵盖人类基因组的39 Mb靶标区域（19,396个基因），并覆盖51 Mb的端到端平铺空间。xGen阻断寡核苷酸（货号1075474、1075475或1075476，用于TruSeq文库），和xGen Lockdown Reagents（货号1072280或1072281）。
了解有关IDT xGen Exome Research Panel的更多信息
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NextSeq 550 System | Samples per run: mid output: 3, high output: 12 (based on 50 million reads, 50× coverage, 4 Gb of data) | 2 × 75 bp (max recommended) |
| HiSeq 2500 System | Samples per run (dual flow cell): rapid run: 24, high output: 156 (based on 50 million reads, 50× coverage, 4 Gb of data) | 2 × 75 bp (max recommended) |
| NovaSeq 6000 System | Samples per run (dual flow cell): S1: 160, S2: 320, S4: 769 (based on 50 million reads, 50× coverage, 4 Gb of data) | ≤ 2 × 100 bp |
| TruSeq DNA Exome | Nextera DNA Exome | AmpliSeq for Illumina Exome Panel | Nextera Flex for Enrichment | |
|---|---|---|---|---|
| Assay Time | 2.5 days | < 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | ~6.5 hours | |
| Content Specifications | Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). | > 97% of CCDS | Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Custom: 0.5 - 15 Mb genomic content of interest. Fixed panels: Content varies by panel. |
|
| Description | A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. | Enables researchers to prepare 8 exome libraries in <7.5 hours with <1.5 hours hands-on time. | A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing. | |
| Hands-On Time | 6 hours | < 1.5 hours | ~2 hours | |
| Input Quantity | 100 ng genomic DNA | 50 ng minimum | 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide). | |
| Mechanism of Action | Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes | Multiplex PCR | Bead-bound transposomes and hybrid-capture chemistry | |
| Method | Exome Sequencing | Exome Sequencing | Amplicon Sequencing , Exome Sequencing | Custom Sequencing, Exome Sequencing , 靶向DNA测序 , 靶向富集 |
| Multiplexing | Up to 12-plex enrichment | 96 dual index combinations | Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. | |
| On-Target Reads | >80% | >80% | ≥85% (for exome panel) | |
| Specialized Sample Types | FFPE Tissue | Low-Input Samples | 不兼容FFPE | Blood, FFPE Tissue, Saliva |
| Species Category | 人类 | 人类 | 人类 | Other, 人类 |
| Uniformity | >85% (% coverage at 20x for 4 Gb) | >90% (% coverage at 20x for 4 Gb) | ≥90% (% coverage at 20x for 4 Gb; for exome panel) |
Dr. James Knowles trusts the HiSeq System and TruSeq library preparation kits for cost-effective, large-scale collaborative projects.
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TruSeq Exome Kit可获得匹配率≥80%的测序read，实现高效、经济的外显子组测序。
| Catalog Number | Total Samples | Number of Enrichment Reactions | Index Adapters |
|---|---|---|---|
| 20020614 | 24 | 8 | 24 single indexes (Set A and Set B) |
| 20020615 | 96 | 8 | 96 combinatorial dual indexes |
Evaluating DNA Quality from FFPE Samples
Technical Note | PDF< 1 MB
Illumina Exomes Comparative Table
Product Information Sheet | PDF< 1 MB
Data Sheet | PDF | 5 versions
TruSeq DNA Exome Consumables & Equipment Documentation
TruSeq DNA Exome Checklist Documentation
TruSeq Exome Library Prep Reference Guide Documentation
Sequencing Library qPCR Quantification Guide Documentation
TruSeq DNA Exome Reference Guide Documentation
TruSeq Exome Library Prep Checklist Documentation
TruSeq Exome Library Prep Consumables and Equipment List Documentation
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