TruSight Hereditary Cancer Panel

Targeted sequencing panel that assesses germline mutations across 113 genes and 125 SNPs associated with genetic cancer risk predisposition.

~48 hr from DNA to data

Assay time

~2 hr

Hands-on time

50–1000 ng DNA 

Input quantity

See full details in the specifications table

Overview

The TruSight Hereditary Cancer Panel delivers expert-defined content for assessing germline mutations.

  • Extensive panel includes 113 genes associated with hereditary cancer predisposition

  • Easy protocol enables fast and flexible library prep and enrichment

  • Compatibility with all Illumina benchtop sequencing systems with sample throughput ranging from 2 to 256 samples per run

  • Hybrid–capture enrichment enables good coverage uniformity for accurate detection of SNVs, indels, and CNVs

Expert-defined content

The panel was developed with input from key opinion leaders and includes 10,341 probes across 113 genes and 125 SNPs.

Fast library prep and enrichment workflow

The panel is used with the Illumina DNA Prep with Enrichment library prep workflow, which takes advantage of enzyme-based DNA fragmentation, removing the instrument cost and need for mechanical fragmentation. Illumina DNA Prep with Enrichment has been qualified with multiple automation systems for a user-friendly workflow.

Scalable

Indexes available for up to 384 samples allow for flexible batch sizes and scalability across Illumina benchtop sequencing systems.


Specifications


Required products

The TruSight Hereditary Cancer Panel workflow requires the TruSight Hereditary Cancer enrichment oligos, Illumina DNA Prep with Enrichment, Illumina DNA/RNA UD indexes, and sequencing reagents.

/ Results

Applications

TruSight Hereditary Cancer Panel is a highly efficient targeted next-generation sequencing (NGS) panel that enables researchers to access an expert-defined content set for analyzing variation within genes previously linked with a predisposition towards cancer.

Example workflow

1
Prep

TruSight Hereditary Cancer – Enrichment Oligos

Illumina DNA Prep with Enrichment

Illumina DNA/RNA UD Indexes


Project recommendations

Instrument Recommended number of samples Read length
iSeq 100 System

Samples per run: 2 (based on 300× mean coverage)

2 × 150 bp (max recommended)

MiniSeq System

Samples per run: mid-output: 5, high-output: 16 (based on 300× mean coverage)

2 × 150 bp (max recommended)

MiSeq System

Samples per run: v2 Micro: 2, v2 Standard: 9, v3 Standard: 16 (based on 300× mean coverage)

2 × 150 bp (max recommended)

NextSeq 500 System

Samples per run: mid-output: 80, high-output: 256 (based on 300× mean coverage)

2 × 150 bp (max recommended)

NextSeq 550 System

Samples per run: mid-output: 80, high-output: 256 (based on 300× mean coverage)

2 × 150 bp (max recommended)


How others use this product

Figures

TruSight Hereditary Cancer Panel gene content

Includes 10,341 probes that target 113 genes related to cancer predisposition, recommended in key guidelines, and evaluated on population studies of cases vs. controls.

Genes included in key guidelines associated with risk reduction

The TruSight Hereditary Cancer Panel includes an extensive list of genes commonly associated with hereditary predisposition to breast, colon, ovarian, and gastric cancers and recommended in key guidelines. 

Library prep (5)

TruSight Hereditary Cancer – Enrichment Oligos Only (8 Enrichment Reactions)

20029551

TruSight Hereditary Cancer – Enrichment Oligos Only (8 Enrichment Reactions)

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)

20025524

Includes reagents for preparing and enriching 96 libraries (eight, 12-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)

20025523

Includes reagents for preparing and enriching 16 libraries (16, 1-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina® DNA Prep, (S) Tagmentation (96 Samples)

20025520

Includes reagents for preparing 96 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (96 samples)).

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina® DNA Prep, (S) Tagmentation (16 Samples)

20025519

Includes reagents for preparing 16 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (16 samples)).

Sign in to add to cart or see pricing.

List Price:

Discounts:

Index adapters (8)

Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20091654

Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20091656

Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20091658

Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20091660

Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

IDT® for Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20042666

Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

IDT® for Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20042667

Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Reagents (10)

iSeq 100 i1 Reagent v2 (300-cycle)

20031371

Includes one i1 flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 300-cycle run on the iSeq 100 System.

Sign in to add to cart or see pricing.

List Price:

Discounts:

iSeq 100 i1 Reagent v2 (300-cycle) 4 pack

20031374

Includes four i1 flow cells and four pre-filled cartridges containing clustering and sequencing reagents to support 4 × 300-cycle runs on the iSeq 100 System.

Sign in to add to cart or see pricing.

List Price:

Discounts:

iSeq 100 i1 Reagent v2 (300-cycle) 8 pack

20040760

Includes eight i1 flow cells and eight pre-filled cartridges containing clustering and sequencing reagents to support 8 × 300-cycle runs on the iSeq 100 System.

Sign in to add to cart or see pricing.

List Price:

Discounts:

MiSeq Reagent Micro Kit v2 (300-cycles)

MS-103-1002

Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Micro Flow Cell (up to 4M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Micro Flow Cell, and Wash Buffer.

Sign in to add to cart or see pricing.

List Price:

Discounts:

MiSeq Reagent Kit v2 (300-cycles)

MS-102-2002

MiSeq v2 Reagent kit, 300 cycles per kit.

Sign in to add to cart or see pricing.

List Price:

Discounts:

MiSeq Reagent Kit v3 (600-cycle)

MS-102-3003

Provides kitted reagents for up to 625 cycles of sequencing on the MiSeq System. Includes: Paired-End Reagent plate (600-cycles), MiSeq Flow Cell, and Wash Buffer.

Sign in to add to cart or see pricing.

List Price:

Discounts:

MiniSeq Mid Output Kit (300-cycles)

FC-420-1004

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a Mid Output run (up to 8M reads). Includes: Mid Output Reagent Cartridge (300 cycles), Flow Cell, and Hybridization Buffer.

Sign in to add to cart or see pricing.

List Price:

Discounts:

MiniSeq High Output Reagent Kit (300-cycles)

FC-420-1003

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a High Output run (up to 25M reads). Includes: High Output Reagent Cartridge (300 cycles), Flow Cell, and Hybridization Buffer.

Sign in to add to cart or see pricing.

List Price:

Discounts:

NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles)

20024905

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a Mid Output run (up to 130M reads). Includes: Mid Output Reagent Cartridge (300 cycles), Mid Output Flow Cell Cartridge, and Buffer Cartridge.

Sign in to add to cart or see pricing.

List Price:

Discounts:

NextSeq 500/550 High Output Kit v2.5 (300 Cycles)

20024908

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a High Output run (up to 400M reads). Includes: High Output Reagent Cartridge (300 cycles), High Output Flow Cell Cartridge, and Buffer Cartridge.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Accessory products (1)

Flex Lysis Reagent Kit (96 reactions)

20018706

Includes reagents for processing blood samples with the Illumina DNA Prep and Illumina DNA Prep with Enrichment. Purchase library prep, enrichment, enrichment probe panel, and index adapter reagents separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Showing of

Selection summary

Product

Qty

Unit Price

FAQs

The TruSight Hereditary Cancer Panel includes an extensive list of genes commonly associated with hereditary predisposition to breast, colon, ovarian, and gastric cancers.

The TruSight Hereditary Cancer Panel can be run on any benchtop Illumina sequencing system (iSeq 100, MiSeq, MiniSeq, and NextSeq Systems).

TruSight Hereditary Cancer Panel includes 125 SNPs. Of these, 48 are ID SNPs and 77 are used for polygenic risk scoring (PRS).

/ Results

Order the NovaSeq X Series

Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.

MiSeq Reagent Kit v3

Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

Speak with a specialist

Reach out for information about our products and services, or get answers to questions about our technology.