Achieve cost-effective, accurate, and sensitive RNA exome analysis of even difficult samples without sacrificing gene fusion discovery power. Many RNA exome sequencing methods focus on a defined number of known transcripts or require expensive deep sequencing. RNA exome capture sequencing overcomes these challenges by combining RNA-Seq with exome enrichment.
This method captures only the coding regions of the transcriptome, allowing higher throughput and requiring lower sequencing depth than non-exome capture methods. Sequence-specific capture of the RNA exome does not rely on the presence of a poly-A tail. This makes RNA exome capture sequencing ideal for RNA-Seq with low-quality samples or limited starting material.
Isolating transcriptome coding regions maximizes discovery power at a fraction of the read depth of total RNA sequencing.
Illumina Distinguished Scientist Dr. Gary Schroth demonstrates the latest advances in RNA-Seq technology. Learn how the latest RNA library prep methods allow transcriptome analysis from challenging samples, like single cells or FFPE tissues.View Video
Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted NGS technology, generating more than 90% of global sequencing data.*
In addition to industry-leading data quality, Illumina offers integrated RNA exome capture workflows that simplify the entire process, from library preparation to data analysis and biological interpretation.
Explore genomics-based approaches to investigating cancer using NGS. Find tools to help you uncover novel insights into the biology of cancer. Learn more about cancer genomics research.
Find NGS solutions to advance disease research of autoimmune and rheumatic diseases, atherosclerosis and many forms of heart disease, neurological disorders, psychiatric disorders and more on a molecular level. and more. Learn more about complex disease research.
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This cost-effective, flexible workflow measures gene expression in single cells and offers high-resolution analysis to discover cellular differences usually masked by bulk sampling methods. This app note outlines the workflow, provides example study designs and throughput charts, and more.
*Data calculations on file. Illumina, Inc., 2015