Illumina DNA Prep
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
MiSeq i100 Series Reagent Kits deliver flexible, efficient, and sustainable workflows.
MiSeq i100 Series Reagent Kits
Reagents for the MiSeq i100 Series feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.
Overview
Key features
Innovative, room temperature storage
These kits offer streamlined, load-and go reagents and room temperature shipping and storage. This innovation removes the requirement for cold chain logistics and freezer storage, delivering remarkable benefits in sustainability and user experience. Increase sample turnaround time and reduce run inconsistencies by eliminating thawing.
Powered by XLEAP-SBS chemistry
The MiSeq i100 Series is powered by XLEAP-SBS chemistry, our fastest, most robust, and highest-quality sequencing by synthesis (SBS) chemistry. It provides significant improvements in stability, speed, and performance across Illumina kits, delivering higher confidence in generated data and expediting project completion.
Specifications
| Instruments | MiSeq i100 Plus System, MiSeq i100 System |
|---|---|
| Nucleic acid type | DNA, RNA |
| Reagent type | Paired-end sequencing, Single-read sequencing |
| Technology | Sequencing |
Required products
Each reagent kit contains the required products for a single flow cell sequencing run. For sequencing runs requiring custom primers, index and read primers are available for purchase (sold separately).
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Results
Documentation
Product literature
Literature is not currently available for this product.
Support documentation
A dedicated support section is not currently available for this product
Instrument (1)
MiSeq i100 Plus System
20115695
The MiSeq i100 Plus System is a benchtop sequencing system that integrates sequencing, primary analysis and secondary analysis. The system includes embedded touchscreen monitor and on-instrument computer, control software, hardware-accelerated DRAGEN secondary analysis pipelines, and 12 months warranty (including parts and labor). The system is self-installable by the customer and does not include installation services.
Reagents (5)
MiSeq i100 Series 5M Reagent Kit (300 cycles)
20126565
This reagent kit provides a 5M flow cell and a reagent consumable for 300 cycles run on MiSeq i100 and MiSeq i100 Plus systems
MiSeq i100 Series 5M Reagent Kit (600 cycles)
20126566
This reagent kit provides a 5M flow cell and a reagent consumable for 600 cycles run on MiSeq i100 and MiSeq i100 Plus systems
MiSeq i100 Series 25M Reagent Kit (100 cycles)
20126567
This reagent kit provides a 25M flow cell and a reagent consumable for 100 cycles run on MiSeq i100 and MiSeq i100 Plus systems
MiSeq i100 Series 25M Reagent Kit (300 cycles)
20126568
This reagent kit provides a 25M flow cell and a reagent consumable for 300 cycles run on MiSeq i100 and MiSeq i100 Plus systems
MiSeq i100 Series 25M Reagent Kit (600 cycles)
20115696
This reagent kit provides a 25M flow cell and a reagent consumable for 600 cycles run on MiSeq i100 and MiSeq i100 Plus systems
Accessory products (1)
PhiX Control v3
FC-110-3001
Kitted DNA control for the Illumina sequencing platform. Compatible with Single and Paired End reads up to 150 base pairs. (10ul of 10nM template solution)
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FAQs
The MiSeq i100 Series Reagent Kits use integrated cartridges that include sequencing reagents and the flow cell, simplifying library loading and improving efficiency throughout the sequencing run helping minimize errors. Easy maintenance with cartridge-based fluidics minimizes cross-contamination between samples and runs. The cartridges have toolless disassembly, recyclable plastics, and are formamide-free.
The MiSeq i100 Series has multiple reagent configurations supporting a variety of applications and sample throughputs. Each configuration will have different run times. Run times range from ~4-15.5 hr.
See the MiSeq i100 Series specifications for more information.
The MiSeq i100 Series can generate up to 100M single reads and 200M paired-end reads, depending on the flow cell type.
See the MiSeq i100 Series specifications for more information.
The MiSeq i100 Series offers up to 10 configurations with four flow cell types. The 5M and 25M flow cells are available on the MiSeq i100 System; the 5M, 25M, 50M, and 100M flow cells are available on the MiSeq i100 Plus System. The 5M and 25M flow cells are available now; the 50M and 100M flow cells will be available in 2025.
See the MiSeq i100 Series specifications for more information.
The MiSeq i100 System is a lower cost instrument that runs 5M and 25M flow cells to support lower data output and sample throughputs. The MiSeq i100 Plus System accesses a wider range of throughputs with 5M, 25M, 50M, and 100M flow cells for more sequencing per sample and larger studies. The MiSeq i100 Plus System with 5M and 25M flow cells are available now. The MiSeq i100 System, and 50M and 100M flow cells are available starting in 2025.
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Results
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