TruSeq Stranded Total RNA with Ribo-Zero Plant

Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep workflow. Remove rRNA from plant leaf, seed, and root tissue.

The product configuration for Cat. No. RS-122-2401, RS-122-2402, and RS-122-2403 has changed. In the new configuration, the library preparation reagents and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.

Overview

High-quality whole-transcriptome analysis with precise strand information

RNA-Seq technology provides uniform coverage, precise measurement of strand orientation, and high-confidence discovery of features such as alternative transcripts, antisense expression, and allele-specific expression across both coding and multiple forms of noncoding RNA. TruSeq Stranded Total RNA with Ribo-Zero Plant couples the benefits of TruSeq RNA library preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable solution for whole-transcriptome analysis.

Efficient ribosomal RNA reduction across a range of plant species, study designs

TruSeq Stranded Total RNA with Ribo-Zero Plant enables rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. These products have been validated for use in multiple plant species, including Arabidopsis thaliana, rice, and maize, and will provide efficient rRNA removal in a broad range of additional species.


Specifications


Required Products

Illumina now offers modular product ordering to enable flexibility in your workflows. You may order any of the following library prep products with any of the following indexes. 

/ Results

Applications

Example workflow


Project recommendations

Instrument Recommended number of samples Read length
NextSeq 550 System

RNA Profiling: 6-20 samples per run (based on 20 million reads per sample)

Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)

2 x 75 bp

2 x 75 bp

NovaSeq 6000 System

RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads.

Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.

≤ 2 × 100 bp

≤ 2 × 100 bp


Related applications and methods

How others use this product

Library prep (2)

TruSeq® Stranded Total RNA Library Prep Plant (48 Samples)

20020610

Includes RiboZero beads to deplete cytoplasmic, mitochondrial, and chloroplast rRNA from plant samples, PCR master mix to transcript RNA into cDNA, and reagents for preparing 48 samples. Purchase index adapters and Purification Beads separately.

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TruSeq® Stranded Total RNA Library Prep Plant (96 Samples)

20020611

Includes RiboZero beads to deplete cytoplasmic, mitochondrial, and chloroplast rRNA from plant samples, PCR master mix to transcript RNA into cDNA, and reagents for preparing 48 samples. Purchase index adapters and Purification Beads separately.

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Index adapters (3)

IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)

20020591

New Unique Dual Indexes (UDIs), compatible with TruSeq Stranded mRNA and TruSeq Stranded Total RNA Library Prep Kits. The UDI kit is provided in a plate format, contains 24 UDIs (x4), sufficient for 96 samples.

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IDT for Illumina – TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples)

20040870

Includes 96, 8 bp indexes sufficient for labeling 96 samples and is compatible with TruSeq Nano and TruSeq PCR-Free Library Prep Kits. Version 2 contains improvements for 8 of the 192 index oligos in plate 20022370, with no changes to the remaining 184. Purchase library preps and probe panels separately.

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IDT for Illumina – TruSeq RNA UD Indexes v2 (96 Indexes, 96 Samples)

20040871

Includes 96, 8 bp indexes sufficient for labeling 96 samples and is compatible with TruSeq Stranded mRNA and TruSeq Stranded Total RNA Library Prep Kits. Version 2 contains improvements for 8 of the 192 index oligos in plate 20022371, with no changes to the remaining 184. Purchase library preps and probe panels separately.

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Selection summary

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