DNA methylation dynamics and dysregulation delineated by high-throughput profiling in the mouse

In this webinar, Dr. Peter Laird from the Van Andel Institute presents a validation study of the Infinium Mouse Methylation BeadChip (version MM285)—a mouse DNA methylation array that contains 296,070 probes representing the diversity of mouse DNA methylation biology—and demonstrates how this array is widely accessible to the research community and can accelerate high sample-throughput studies in this important model organism.

Within his discussion, Dr. Laird covers applications for comparative epigenomics, genomic imprinting, epigenetic inhibitors, PDX assessment, backcross tracing, and epigenetic clocks.

What you will learn in this webinar:

• Detailed description of the design principles and content for the 296,070 probes on the array
• A mouse DNA methylation atlas representing 1,239 samples with data uploaded to GEO
• Guidance for data processing and analysis through the SeSAMe tool
• Application to low input DNA samples (5 ng) and challenging samples, such as FFPE
• Biological validation with epigenetic inhibitors and DNA methyltransferase knockout mice
• Development of tissue-specific methylation signatures for reference-based tissue deconvolution
• Demonstration of applications in human PDX sample purity assessment
• Application to more than 20 strains of mice
• A cross-validated epigenetic clock that is applicable across tissue types

Peter W. Laird, PhD
Professor
Van Andel Institute

M-US-00314