AmpliSeq for Illumina Sequencing Solution

AmpliSeq for Illumina is a suite of AmpliSeq chemistry products that are compatible with Illumina next-generation sequencing platforms. The AmpliSeq for Illumina solution offers a highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds in a single run.

Combined with Illumina next-generation sequencing (NGS) technology, AmpliSeq for Illumina sequencing offers high-confidence data to researchers in a wide variety of application areas, including cancer and genetic diseases. AmpliSeq for Illumina works with DNA and RNA samples and requires as little as 1 ng of input.

AmpliSeq panels can accommodate high-quality samples such as blood, cell culture, or fresh frozen tissue and also challenging samples such as formalin-fixed paraffin-embedded (FFPE) tissue. Select from predesigned panels or customize your content for a variety of genomes and unmatched flexibility.

AmpliSeq for Illumina products are only sold by Illumina and the complete workflow, from design to analysis, is supported by Illumina.

Sequencing Amplified: AmpliSeq for Illumina

Learn how AmpliSeq for Illumina panels provide a simple, fast, and robust sequencing option to achieve high-confidence data.

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Expand Your Research Capabilities

Sequence with innovative NGS platforms that deliver exceptional data quality and accuracy, at any scale, with low hands-on time:

Target variants for many applications with ready-to-use or custom panels
Use a multiplexed, PCR-based workflow
Transform data into insight with a variety of data analysis solutions

Sequence a Broad Range of Samples

AmpliSeq for Illumina is compatible with samples where available input quantity and quality are not limiting, such as blood, cell culture, or fresh-frozen tissues as well as challenging sample types, such as formalin-fixed paraffin-embedded (FFPE) tissue. The input requirement for DNA and RNA ranges from 1 ng to 100 ng, depending on the application needs. For most applications, we recommend 10 ng input per pool.

Prepare Libraries Quickly and Easily

Library preparation with AmpliSeq for Illumina is both fast and simple. Prepare an on-target, high-uniformity amplified library in as little as 5 hours with just 1.5 hours of hands-on time.

Analyze Hundreds of Genes at Once

AmpliSeq chemistry allows researchers to analyze hundreds of genes simultaneously with ultra-high multiplexed PCR with 12 to more than 24,000 amplicons in a single panel. Achieve complete coverage of large targets using multiple primer pools to create overlapping amplicons.

Introducing AmpliSeq for Illumina

Learn how AmpliSeq for Illumina provides researchers with a high-confidence solution to detect variants with flexible input ranges and sample types.

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AmpliSeq for Illumina Oncology Panels

This application note demonstrates the performance of the iSeq 100 System with four distinct targeted resequencing panels.

Automated Library Prep Methods

Achieve consistent, reproducible library prep on AmpliSeq for Illumina panels with automation solutions from our trusted partners.

Accurate Sample Identification

Adding this optional product to any AmpliSeq for Illumina human DNA panel can help you minimize sample misidentification.

Prepare high-quality libraries quickly and simply using a multiplexed, PCR-based workflow

Content Selection

AmpliSeq for Illumina Ready-to-Use Panels

AmpliSeq for Illumina Custom Panels

AmpliSeq for Illumina Community Panels

AmpliSeq for Illumina On-Demand Panels

Library Prep & Accessories

5-7 hours of total time
1.5 hours hands-on

Library PLUS, Indexes, and Accessories

Sequencing

17-32 hours

Benchtop Sequencers

Data Analysis

Time varies

AmpliSeq for Illumina Informatics Solutions

Frequently Purchased Together

AmpliSeq for Illumina panels are optimized for clinical cancer and genetic disease research applications. Available as ready-to-use, community, custom, or on-demand panels.

Ready-to-Use Panels

Predesigned panels target the variants most commonly seen in each area of interest, and are for use with DNA or RNA. Available in convenient pack sizes, usually consisting of 24 reactions.

Community Panels

Made-to-order panels containing content selected and designed with input from leading disease researchers. Predesigned amplicons offer a fast, easy way to get started. Species compatibility varies.

Custom Panels

Made-to-order panels use coordinates you provide to target genomic regions of interest. Use preloaded reference genomes or upload a reference genome. Available for human and other species.

On-Demand Panels

Made-to-order panels with just the genes you’re interested in. Select from over 5000 pretested genes relevant for inherited disease research. Available in convenient, small pack sizes of 24 or 96 reactions.

Looking for other types of cancer research panels?

Explore All Cancer Panels by Application Workflow

AmpliSeq for Illumina Targeted Resequencing Solution

A streamlined, scalable amplicon sequencing solution, producing high-confidence data from low-input DNA and RNA samples.

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Accurate Data Across Various Sample Types

AmpliSeq for Illumina Focus Panel allows oncology researchers to target hotspots, single nucleotide polymorphisms (SNVs), indels, copy number variation (CNVs), and gene fusions from DNA or RNA in a single workflow.

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AmpliSeq for Illumina FAQs

Wondering how the various AmpliSeq panels for Illumina sequencing differ? Have questions about which components you’ll need, panel customization and post-sequencing analysis options, or where to go for technical support?

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Clinical Cancer Research

Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.

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For every lab, everywhere

NextSeq 1000 and NextSeq 2000 Reagents

NGS Workflow Finder

DRAGEN v4.3 now available on-premises and on-cloud

Illumina Proactive Instrument Performance Service

Do more, faster than ever

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

This is the Genome Era

This is the Genome Era

This is the Genome Era

This is the Genome Era

This is the Genome Era

This is the Genome Era

This is the Genome Era

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

AmpliSeq for Illumina – a comprehensive targeted resequencing solution

Fast workflow and robust performance from a few to hundreds of genes, with as little as 1 ng input

Highly Accurate Data with a Fast, Simple Workflow

Sequencing Amplified: AmpliSeq for Illumina

Sequencing Amplified: AmpliSeq for Illumina

Quality and Ease of Use

Expand Your Research Capabilities

Sequence a Broad Range of Samples

Prepare Libraries Quickly and Easily

Analyze Hundreds of Genes at Once

Introducing AmpliSeq for Illumina

Sequencing Amplified: AmpliSeq for Illumina

Related AmpliSeq for Illumina Applications

AmpliSeq for Illumina Oncology Panels

Automated Library Prep Methods

Accurate Sample Identification

AmpliSeq for Illumina Sequencing Solution