AmpliSeq for Illumina Custom DNA Panel
Targeted custom research panels optimized for sequencing specific targets or genomic content of interest.
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Panel
Library Prep
Index Adapters
Services
AmpliSeq for Illumina DNA Library Prep Training
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Product Highlights
Accurate Data Across Various Sample Types
Achieve unmatched data quality with AmpliSeq chemistry and Illumina next-generation sequencing (NGS) technology, even from low-quality starting materials such as formalin-fixed, paraffin-embedded (FFPE) tissues.
Include only the genes and targets of interest
- Easily design custom content with DesignStudio, a free, user-friendly, online tool that creates panels optimized for the targets or specific genomic content of interest.
- Flexible and tunable designs from 12 to over 12,000 amplicons, and multiple amplicon size options.
Fast, Simple Workflow
- Increased efficiency with a 5.5-hour library preparation workflow with only 1.5 hours of hands-on time.
Scalable Solution
- Multiple options for sequencing platforms, data analysis, and support meet virtually any throughput requirements.
The AmpliSeq for Illumina Custom DNA Panel is the recommended replacement for the TruSeq Custom Amplicon and TruSeq Custom Amplicon Low-Input kits, which have been discontinued.
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Specifications
Product Comparison
| AmpliSeq for Illumina Custom DNA Panel | AmpliSeq for Illumina On-Demand | Illumina DNA Prep with Enrichment | |
|---|---|---|---|
| Content Specifications | Custom content of interest - up to 5 Mb | Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes | Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Custom: 0.5 - 15 Mb genomic content of interest. Fixed panels: Content varies by panel. |
| Description | A custom research assay that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. | A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. | A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing. |
| Input Quantity | 1–100 ng (10 ng recommended per pool) | 1-100 ng DNA | 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide). |
| Mechanism of Action | Multiplex PCR | Multiplex PCR | Bead-bound transposomes and hybrid-capture chemistry |
| Method | Amplicon Sequencing, Custom Sequencing, Genotyping by Sequencing, Targeted DNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing | Custom Sequencing, Exome Sequencing, Target Enrichment, Targeted DNA Sequencing |
| Multiplexing | Up to 96-plex | Up to 96-plex | Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. |
| Number of Reactions | 750 or 3000 reactions, 750 or 3000 reactions | 24 or 96 reactions | Custom workflow: 16 or 96 samples |
| Specialized Sample Types | Blood, FFPE Tissue | Blood, FFPE Tissue | Blood, FFPE Tissue, Saliva |
| Species Category | Any Species, Bovine, Canine, Chicken, Hamster, Human, Maize, Mouse, Ovine, Porcine, Rice, Soybean, Tomato | Human | Human, Other |
DNA Amplicon LRM Module
Product Literature
Application Note | PDF | 5 versions
Manuals and Support Information
Illumina Adapter Sequences Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina Custom and Community Panels DNA Checklist Documentation
AmpliSeq for Illumina Custom and Community Panels Documentation
AmpliSeq for Illumina Custom and Community Panels RNA Checklist Documentation
AmpliSeq for Illumina Custom and Community Panels Consumables & Equipment Documentation
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Custom and Community Panels Reference Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All AmpliSeq for Illumina Custom and Community Panels Support
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MiSeq Reagent Kits v2
MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.