Targeted custom enrichment panels, providing simple and efficient solutions for multiple targeted sequencing applications.
Illumina Custom Enrichment Panel v2 provide fast, flexible content for targeted sequencing applications, enabling a fully customized enrichment solution.
| Description | Fully customized enrichment panels with speed, flexibility, and scalability for targeted sequencing applications. |
|---|---|
| Instruments | NovaSeq 6000Dx Instrument, MiSeq System, MiSeqDx Instrument, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System |
| Library prep compatibility | Illlumina Cell-Free DNA Prep with Enrichment, Illumina Complete Long Reads with Enrichment, Human, Illlumina DNA Prep with Enrichment |
| Method | Target enrichment |
| Nucleic acid type | DNA |
| Species category | Any species, Other, Human |
| Technology | Sequencing |
Illumina Custom Enrichment Panel v2 is a 120 bp, double-stranded probe panel designed using DesignStudio and includes enrichment oligos for hybridization capture. Library prep, enrichment and index adapter reagents must be purchased separately.
Illumina Custom Enrichment Panel v2 is compatible with multiple targeted enrichment workflows. With DesignStudio, panels can be designed for use with multiple Illumina library preps:
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Custom panel sample calculations assume the indicated target coverage depths using 120 bp double-stranded DNA oligo panels (Illumina Custom Enrichment Panel v2) or 80-mer single-stranded dNA oligo panels (Illumina Custom Enrichment Panel) for target capture at 80% enrichment efficiency and 2 x 101 bp read length chemistry. Number of samples may vary depending on workflow handling, input sample or library quality, and actual sequencing output of each platform and flow cell.
| Part Number | Name | Volume (µl) | # of Samples: Illumina DNA Prep with Enrichment | # of Samples: Illumina Cell-Free DNA Prep with Enrichment | # of Samples: Illumina Complete Long Reads Prep with Enrichment |
|---|---|---|---|---|---|
| 20073953 | Illumina Custom Enrichment Panel v2 (32 µl, 120 bp) | 32 µl | 96 | 8 (1-plex) or 32 (4-plex) |
64 |
| 20073952 | Illumina Custom Enrichment Panel v2 (384 µl, 120 bp) | 384 µl | 1152 | 96 (1-plex) or 384 (4-plex) |
768 |
| 20111339 | Illumina Custom Enrichment Panel v2 (1536 µl, 120 bp) | 1536 µl | 4608 | 384 (1-plex) or 1536 (4-plex) |
3072 |
| Panel Volume per Hyb | 4 ul probe panel per hyb rxn | 4 ul probe panel per hyb rxn | Dilute probe panel 5-fold, use 2.5 ul per hyb | ||
| Plexity | 12 samples per hyb* | 1 sample or 4 samples per hyb | 1 sample per hyb |
| Illumina Custom Enrichment Panel v2 | Illumina Custom Enrichment Panel | |
|---|---|---|
| Panel size, # of probes | 100 to 1,000,000 | 2000 to 67,000 |
| Oligo format | 120 bp double-stranded oligo probes | 80-mer single-stranded oligo probes |
| Available dispense sizes | 32 µl, 384 µl, 1536 µl | 80 µl |
| >99.9% of probes present, confirmed by NGS | Yes | No |
| Compatible Illumina Library Preps | Illumina DNA Prep with Enrichment, Illumina Cell-Free DNA Prep with Enrichment, Illumina Exome 2.5 Enrichment, Illumina Complete Long Reads Enrichment | Illumina DNA Prep with Enrichment, Illumina Cell-Free DNA Prep with Enrichment |
| Design Tool | DesignStudio or Concierge Services | DesignStudio or Concierge Services |
| Turn-around time* | 3-6 weeks | 6-8 weeks |
| Panel Cost | $ | $$$ |
*additional ship time may be needed for non-US destinations
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Illlumina has supported protocols for use of Illumina Custom Enrichment v2 panels with Illumina DNA Prep with Enrichment and Illumina DNA Prep with Exome 2.5 Enrichment, Illumina cfDNA Prep with Enrichment and Illumina Complete Long Reads with Enrichment workflows.
| Illumina DNA Prep with Enrichment | Illumina DNA Prep with Exome 2.5 Enrichment | Illumina cfDNA Prep with Enrichment | Illumina Complete Long Reads with Enrichment | |
|---|---|---|---|---|
| Minimum panel size | 500 probes | 100 probes (when combined with Exome 2.5) | 70kb or 700 probes, for double-stranded probes | 2,500 (0.3Mb panel with 190 probes was tested however PRE=34.8%) |
| Supported plexities | 1-plex or 12-plex | 12-plex | 1-plex or 4-plex | 1-plex |
| Volume of Panel used | 4 µl per enrichment reaction | 4 µl per enrichment reaction | 4 µl per enrichment reaction | Dilute panel 5-fold, use 2.5 µl per enrichment reaction |
| # of samples supported (20073952 Illumina Custom Enrichment Panel v2 (32 µl, 120-bp) |
|
96 samples at 12-plex |
|
64 samples at 1-plex |
| # of samples supported (20073953 Illumina Custom Enrichment Panel v2 (384 µl, 120-bp) |
|
1,152 samples at 12-plex |
|
768 samples at 1-plex |
Illlumina has supported protocols for use of Illumina Custom Enrichment panels with Illumina DNA Prep with Enrichment and Illumina cfDNA Prep with Enrichment workflows.
The sample type compatibility is determined by the library prep kit paired with the custom panel. For Early Access we have supported protocols for use of the panels with Illumina DNA Prep with Enrichment (which is compatible with saliva, blood, purified gDNA and FFPE) and Illumina DNA Prep with Exome 2.5 Enrichment (saliva, blood and purified gDNA). Library prep and enrichment protocols with defined supported sample types will be released with launch of the Illumina cfDNA Prep with Enrichment and Illumina Complete Long Read with Enrichment workflows.
The Illumina Concierge Design Team can help with complex designs, or designs for enrichment panels for non-human targets. This is a free service open to all customers. Contact your Illumina Sales Team representative to get connected to the Concierge team.
Illumina Custom Enrichment Panel V2 (32 µl, 120 bp)
20073953
Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 32 µl of material (sufficient for 8 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately.
Illumina Custom Enrichment Panel V2 (384 µl, 120 bp)
20073952
Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 384 µl of material (sufficient for 96 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately.
Illumina Custom Enrichment Panel V2 (1536 µl, 120 bp)
20111339
Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 1536 µl of material (sufficient for 384 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately.
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