The TruSight Hereditary Cancer Panel delivers expert-defined content for assessing germline mutations.
TruSight Hereditary Cancer Panel
Targeted sequencing panel that assesses germline mutations across 113 genes and 125 SNPs associated with genetic cancer risk predisposition.
~48 hr from DNA to data
Assay time
~2 hr
Hands-on time
50–1000 ng DNA
Input quantity
Overview
Expert-defined content
The panel was developed with input from key opinion leaders and includes 10,341 probes across 113 genes and 125 SNPs.
Fast library prep and enrichment workflow
The panel is used with the Illumina DNA Prep with Enrichment library prep workflow, which takes advantage of enzyme-based DNA fragmentation, removing the instrument cost and need for mechanical fragmentation. Illumina DNA Prep with Enrichment has been qualified with multiple automation systems for a user-friendly workflow.
Scalable
Indexes available for up to 384 samples allow for flexible batch sizes and scalability across Illumina benchtop sequencing systems.
Specifications
| Assay time | ~48 hr from DNA to data |
|---|---|
| Automation capability | Liquid handling robots |
| Cancer type | Pan-cancer, Solid tumor |
| Content specifications | Targets 113 cancer risk-associated genes |
| Description | The TruSight Hereditary Cancer Panel is a targeted sequencing panel designed to assess germline mutations across 113 genes and 125 single nucleotide polymorphisms (SNPs) for identification purposes and polygenic risk scoring. |
| Hands-on time | ~2 hr |
| Input quantity | 50–1000 ng DNA |
| Instruments | MiSeq System, iSeq 100 System, NextSeq 550 System, MiniSeq System, NextSeq 550Dx in Research Mode, NextSeq 500 System |
| Method | Targeted DNA sequencing, Target enrichment |
| Nucleic acid type | DNA |
| Sample type details | Genomic DNA |
| Specialized sample types | Blood, Saliva |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Germline variants, Insertions-deletions (indels), Copy number variants (CNVs) |
Required products
The TruSight Hereditary Cancer Panel workflow requires the TruSight Hereditary Cancer enrichment oligos, Illumina DNA Prep with Enrichment, Illumina DNA/RNA UD indexes, and sequencing reagents.
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Results
Applications
TruSight Hereditary Cancer Panel is a highly efficient targeted next-generation sequencing (NGS) panel that enables researchers to access an expert-defined content set for analyzing variation within genes previously linked with a predisposition towards cancer.
Example workflow
Prep
TruSight Hereditary Cancer – Enrichment Oligos
Illumina DNA Prep with Enrichment
Illumina DNA/RNA UD Indexes
Sequence
Project recommendations
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| iSeq 100 System | Samples per run: 2 (based on 300× mean coverage) |
2 × 150 bp (max recommended) |
| MiniSeq System | Samples per run: mid-output: 5, high-output: 16 (based on 300× mean coverage) |
2 × 150 bp (max recommended) |
| MiSeq System | Samples per run: v2 Micro: 2, v2 Standard: 9, v3 Standard: 16 (based on 300× mean coverage) |
2 × 150 bp (max recommended) |
| NextSeq 500 System | Samples per run: mid-output: 80, high-output: 256 (based on 300× mean coverage) |
2 × 150 bp (max recommended) |
| NextSeq 550 System | Samples per run: mid-output: 80, high-output: 256 (based on 300× mean coverage) |
2 × 150 bp (max recommended) |
How others use this product
Documentation
Product literature
Figures
TruSight Hereditary Cancer Panel gene content
Includes 10,341 probes that target 113 genes related to cancer predisposition, recommended in key guidelines, and evaluated on population studies of cases vs. controls.
Genes included in key guidelines associated with risk reduction
The TruSight Hereditary Cancer Panel includes an extensive list of genes commonly associated with hereditary predisposition to breast, colon, ovarian, and gastric cancers and recommended in key guidelines.
Library prep (5)
TruSight Hereditary Cancer – Enrichment Oligos Only (8 Enrichment Reactions)
20029551
TruSight Hereditary Cancer – Enrichment Oligos Only (8 Enrichment Reactions)
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Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)
20025524
Includes reagents for preparing and enriching 96 libraries (eight, 12-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.
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Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)
20025523
Includes reagents for preparing and enriching 16 libraries (16, 1-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.
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Illumina® DNA Prep, (S) Tagmentation (96 Samples)
20025520
Includes reagents for preparing 96 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (96 samples)).
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Illumina® DNA Prep, (S) Tagmentation (16 Samples)
20025519
Includes reagents for preparing 16 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (16 samples)).
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Index adapters (8)
Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)
20091654
Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)
20091656
Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)
20091658
Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)
20091660
Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)
20027213
Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)
20027214
Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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IDT® for Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)
20042666
Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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IDT® for Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)
20042667
Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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Reagents (10)
iSeq 100 i1 Reagent v2 (300-cycle)
20031371
Includes one i1 flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 300-cycle run on the iSeq 100 System.
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iSeq 100 i1 Reagent v2 (300-cycle) 4 pack
20031374
Includes four i1 flow cells and four pre-filled cartridges containing clustering and sequencing reagents to support 4 × 300-cycle runs on the iSeq 100 System.
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iSeq 100 i1 Reagent v2 (300-cycle) 8 pack
20040760
Includes eight i1 flow cells and eight pre-filled cartridges containing clustering and sequencing reagents to support 8 × 300-cycle runs on the iSeq 100 System.
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MiSeq Reagent Micro Kit v2 (300-cycles)
MS-103-1002
Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Micro Flow Cell (up to 4M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Micro Flow Cell, and Wash Buffer.
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MiSeq Reagent Kit v2 (300-cycles)
MS-102-2002
MiSeq v2 Reagent kit, 300 cycles per kit.
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MiSeq Reagent Kit v3 (600-cycle)
MS-102-3003