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AmpliSeq for Illumina On-Demand

Tailor panel designs for human disease research efficiently and conveniently by selecting from a catalog of over 5,000 pretested genes.

As low as 5 hr (library prep only; doesn't include library quantification, normalization, or pooling time)

Assay time

1.5 hr

Hands-on time

1-100 ng DNA

Input quantity

See full details in the specifications table

Overview

Create amplicon panels with the genes you want. AmpliSeq for Illumina On Demand panels provide a choice of > 5000 pretested genes with known content relevant for inherited disease research, including hereditary cancer, primary immunodeficiency, hearing loss, and muscular dystrophy.

AmpliSeq chemistry and Illumina next-generation sequencing (NGS) technology provide unmatched data quality, even from low-quality starting materials such as formalin-fixed, paraffin-embedded (FFPE) tissues.

Choice of > 5000 pretested genes

  • Easy online ordering - Use DesignStudio Software, a free, user-friendly tool, to create panels optimized for your genomic content of interest.

  • Convenient small pack sizes - On-demand panels are available for 1 (24 amplicons) to 500 (15,000 amplicons) genes and are offered in small pack sizes of 24 and 96 reactions.

Learn more about Ampliseq for Illumina

Specifications

Assay time As low as 5 hr (library prep only; doesn't include library quantification, normalization, or pooling time)
Content specifications Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes
Description A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy.
Hands-on time 1.5 hr
Input quantity 1-100 ng DNA
Instruments MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NextSeq 500 System
Mechanism of action Multiplex PCR
Method Targeted DNA sequencing, Amplicon sequencing
Multiplexing Up to 96-plex
Nucleic acid type DNA
Number of reactions 24 or 96 reactions
Specialized sample types Blood, FFPE tissue
Species category Human
Technology Sequencing
Variant class Single nucleotide polymorphisms (SNPs), Germline variants, Insertions-deletions (indels)

Required products

In addition to an AmpliSeq for Illumina On-Demand Panel, you'll need AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina. 

AmpliSeq for Illumina Direct FFPE DNA is strongly recommended for FFPE samples. The AmpliSeq for Illumina Sample ID Panel is an optional accessory to facilitate sample management.

/ Results

Applications

Example workflow

1
Prep

AmpliSeq for Illumina On-Demand

AmpliSeq for Illumina CD Indexes

2
Sequence
3
Analyze

Related applications and methods

AmpliSeq for Illumina On-Demand Panels

Create amplicon panels with the genes you want. Select from a catalog of pretested genes with known content relevant for inherited disease research.

Cancer Genomics Research

Our sequencing and microarray technologies support a broad range of cancer genomics research applications, from DNA to RNA analysis, epigenetics, and more.

Documentation

Product literature

Literature is not currently available for this product.

Support documentation

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AmpliSeq for Illumina On-Demand AmpliSeq for Illumina Custom DNA Panel Illumina DNA Prep with Enrichment
Assay time As low as 5 hr (library prep only; doesn't include library quantification, normalization, or pooling time) As low as 5 hr (library prep only; doesn't include library quantification, normalization, or pooling time) ~6.5 hr
Content specifications Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes Custom content of interest - up to 5 Mb Custom: 0.5 - 15 Mb genomic content of interest, with Illumina Custom Enrichment Panels and Illumina Custom Enrichment Panel v2
Fixed panels: Content varies by panel.
Description A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. A custom research assay that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. Prepare sequencing libraries for a variety of experiments, including whole-exome, custom, and fixed panel targeted sequencing, from low DNA inputs and a variety of sample types in ~6.5 hours. 
Hands-on time 1.5 hr 1.5 hours ~2 hr, ~2 hr
Input quantity 1-100 ng DNA 1–100 ng (10 ng recommended per pool) 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).
Instruments MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NextSeq 500 System MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System, NovaSeq X Plus System
Mechanism of action Multiplex PCR Multiplex PCR Bead-bound transposomes and hybrid-capture chemistry
Method Targeted DNA sequencing, Amplicon sequencing Targeted DNA sequencing, Custom sequencing, Amplicon sequencing, Genotyping by sequencing Targeted DNA sequencing, Custom sequencing, Exome sequencing, Target enrichment
Multiplexing Up to 96-plex Up to 96-plex Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.
Nucleic acid type DNA DNA DNA
Number of reactions 24 or 96 reactions 750 or 3000 reactions Custom workflow: 16 or 96 samples
Specialized sample types Blood, FFPE tissue Blood, FFPE tissue Blood, Low-input samples, FFPE tissue, Saliva
Species category Human Any species, Tomato, Bovine, Mouse, Canine, Rice, Ovine, Hamster, Chicken, Porcine, Human, Maize, Soybean Other, Human
Technology Sequencing Sequencing Sequencing
Variant class Single nucleotide polymorphisms (SNPs), Germline variants, Insertions-deletions (indels) Single nucleotide polymorphisms (SNPs), Loss of heterozygosity (LOH), Somatic variants, Germline variants, Insertions-deletions (indels), Copy number variants (CNVs)

Panel (6)

AmpliSeq™ for Illumina® On-Demand Panel (24 Reactions, 1-50 Genes)

20023977

Sufficient for 24 reactions and 1–50 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.

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AmpliSeq™ for Illumina® On-Demand Panel (24 Reactions, 51-300 Genes)

20023983

Sufficient for 24 reactions and 51–300 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.

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AmpliSeq™ for Illumina® On-Demand Panel (24 Reactions, 301-500 Genes)

20023978

Sufficient for 24 reactions and 301–500 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.

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AmpliSeq™ for Illumina® On-Demand Panel (96 Reactions, 1-50 Genes)

20023979

Sufficient for 96 reactions and 1–50 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.

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AmpliSeq™ for Illumina® On-Demand Panel (96 Reactions, 51-300 Genes)

20023980

Sufficient for 96 reactions and 51–300 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.

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AmpliSeq™ for Illumina® On-Demand Panel (96 Reactions, 301-500 Genes)

20023981

Sufficient for 96 reactions and 301–500 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.

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Library prep (2)

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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Index adapters (1)

AmpliSeq™ CD Indexes Set A for Illumina®

20019105

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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Accessory products (2)

AmpliSeq™ for Illumina® Sample ID Panel

20019162

Includes eight single nucleotide polymorphism (SNP)-targeting primer pairs and one gender discriminating primer pair sufficient for 96 reactions when paired with AmpliSeq Library PLUS for Illumina. Enables quick and accurate sample identification. Purchase library prep, probe panels, and index adapters separately.

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AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification.

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