This targeted RNA sequencing panel is a cost-effective solution to detect gene fusions in multiple cancer types, regardless of origin. Covering 507 fusion-associated genes, a single assay enables researchers to assess most known cancer-related fusions in blood, bone marrow, and FFPE samples, with the power to identify novel fusion gene partners.
On-instrument fusion calling provides NGS-based fusion detection to labs without additional bioinformatics support. The analysis provides a list of detected fusions, relevant disease associations (as identified by the Mitelman database), and evidence of fusion-supporting reads.
This assay accommodates as little input as 20 ng FFPE RNA or 10 ng fresh-frozen total RNA. The TruSight RNA Fusion Panel provides a sensitive, reproducible, and economical solution for studies of gene fusions in cancer research.
|Instrument||Recommended Number of Samples||Read Length|
|MiniSeq System||Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample)||2 × 76 bp (max recommended)|
|MiSeq System||8 samples per run with v3 reagents (based on 3 million reads per sample)||2 × 76 bp (max recommended)|
|NextSeq 550 System||24 samples per run (mid output; based on 3 million reads per sample)||2 × 76 bp (max recommended)|
|TruSight RNA Fusion Panel||TruSight Tumor 170||AmpliSeq for Illumina Focus Panel||AmpliSeq for Illumina Comprehensive Panel v3|
|Assay Time||2.5 days||~2 days (Library Prep)||5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time)||5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time)|
|Cancer Type||Pan-Cancer||Solid Tumor||Solid Tumor||Solid Tumor|
|Hands-On Time||11 hours||~10.5 hours||< 1.5 hours||< 1.5 hours|
|Method||Target Enrichment, Targeted RNA Sequencing||Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing||Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing||Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing|
|Nucleic Acid Type||RNA||RNA, DNA||DNA, RNA||DNA, RNA|
|Specialized Sample Types||FFPE Tissue, Low-Input Samples||FFPE Tissue, Low-Input Samples||FFPE Tissue||FFPE Tissue|
TruSight RNA Fusion Panel Gene List
product_file | EXCEL < 1 MB
Brochure | PDF 6 MB
Data Sheet | PDF 1 MB
Illumina Experiment Manager User Guide Documentation
Sequencing Library qPCR Quantification Guide Documentation
Local Run Manager RNA Fusion Analysis Module Workflow Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All TruSight RNA Fusion Panel Documentation Support All TruSight RNA Fusion Panel Support