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Illumina and GenoScreen Deeplex® Myc-TB Combo Kit

A targeted NGS-based assay for sequencing mycobacterium, M. tuberculosis complex (MTBC) strains, and antimicrobial resistance markers.

< 48 hr from sample to completed analysis

Assay time

9 pg DNA

Input quantity

See full details in the specifications table

Kits are not available in all countries/regions. Contact your sales representative for availability.

Overview

The Illumina and GenoScreen Deeplex® Myc-TB Combo Kit is a targeted next-generation sequencing panel for the identification of Mycobacterium tuberculosis complex (MTBC) strains, mycobacterial species identification, genotyping, and prediction of drug resistance mutations of MTBC strains. Data analysis is included via a cloud-based Deeplex® software web application with results visualization. Two kit options are available based on which library prep is desired.

  • Identification of resistance mutations to 15 anti-tuberculosis drugs

  • Genotyping and spoligotyping of MTBC strains to determine lineage and sublineage of strains present in a sample

  • Identification of >140 mycobacterial species and detection of co-infections with distinct species

  • Total turnaround time less than 48 hours directly from sputum with no need to perform culture

Combo kits contain either Illumina DNA Prep (IDP) or Nextera XT library prep, plus the Deeplex® Myc-TB multiplex panel, indexes, and bioinformatic analysis. Choose the SKU that includes the library prep you prefer.

Specifications

Assay time < 48 hr from sample to completed analysis
Description PCR panel, library prep, indexes and bioinformatics analysis for identification of mycobacterium tuberculosis and mutations associated with antimicrobial resistance from sputum samples or mycobacterial cultures. Access to detailed interactive cloud-based software included in kit.
Input quantity 9 pg DNA
Instruments MiSeq System, iSeq 100 System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NextSeq 500 System
Mechanism of action Multiplex PCR
Method Targeted DNA sequencing
Multiplexing Varies by instrument
Nucleic acid type DNA
Number of amplicons 24
Species category Bacteria
Technology Sequencing

Applications

Example workflow

1
Prep

Illumina and GenoScreen Deeplex® Myc-TB Combo Kit

2
Sequence
3
Analyze
Deeplex® Myc-TB Web App

Related applications and methods

Tuberculosis sequencing

Next-generation sequencing is advancing the detection, characterization, and surveillance of drug-resistant tuberculosis strains.

Antimicrobial resistance detection

Next-generation sequencing offers high-throughput capabilities to identify genomic changes associated with antimicrobial resistance.

Public health surveillance

Genomics can advance public health and wellness by detecting and characterizing new, emerging, and circulating pathogens.

Documentation

Product literature
Support documentation

Illumina and Genoscreen Deeplex Myc-TB Combo IDP Kit (48 samples)

20099512

Contains Deeplex Myc-TB panel, Illumina DNA Prep (IDP) library prep, unique dual indexes, and Deeplex Web App analysis for 48 samples

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Illumina and Genoscreen Deeplex Myc-TB Combo XT Kit (48 samples)

20090205

Contains Deeplex Myc-TB panel, Nextera XT library prep, Nextera indexes, index adapter caps, and Deeplex Web App analysis for 48 samples

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