Our stranded total RNA library prep solutions are designed for human, mouse, rat and plant sequences, but may be applicable to a variety of eukaryotic species. The following tables list organisms that have been laboratory tested and are fully supported by Illumina, tested in silico, or reported to work by our customers. Illumina cannot guarantee rRNA removal based on customer-reported results.
RNA-Seq uses next-generation sequencing to analyze expression across the transcriptome, enabling researchers to detect known or novel features and quantify RNA.
Learn More| Sample | rRNAs Targeted |
|---|---|
| Human cytoplasmic rRNAs | 28S, 18S, 5.8S, 5S |
| Human mitochondrial rRNAs | 12S, 16S |
| Human beta globin transcripts | HBA1, HBA2, HBB, HBG1, HBG2 |
| Mouse & Rat rRNA | 16S, 28S |
| Gram(-) Bacterial rRNAs | E. coli: 5S, 16S, 23S |
| Gram(+) Bacterial rRNAs | Bacillus subtilis: 5S, 16S, 23S |
| Organism | Laboratory Tested | In silico Tested | Customer Reported | Comments |
|---|---|---|---|---|
| Bee (Apis mellifera) | Use highest quality RNA possible. | |||
| Chicken (Gallus gallus) | Removes 5S, 18S and 28S rRNAs. Mitochondrial rRNAs not tested. | |||
| Ciona intestinalis | Removes 18S rRNA. Other rRNAs not tested. | |||
| Colletotrichum higginsianum | Will remove 28S and 5.8S rRNA. Will not remove 5S rRNA. 18S rRNA not evaluated. | |||
| Copepod species | Removes 28S and 18S rRNAs. Other rRNAs were not evaluated. | |||
| Cow | ||||
| Dictyostelium discoideum | Removes 28S and 18S rRNA from high quality RNA. Will not remove small rRNAs. | |||
| Dog | ||||
| Ferret (Mustela putorius) | ||||
| Finch (Carpodacus mexicanus) | Removes 5S and 18S rRNA. Will not remove 18S rRNA. Mitochondrial rRNAs not tested. | |||
| Flour beetle (Tribolium castaneum) | ||||
| Frog (Xenopus) | ||||
| Fruit Fly (Drosophila) | Will not remove 5S rRNA or 'right' fragment of 28S rRNA. | |||
| Fungus (Aspergillus niger) | 300 nt region of 18S rRNA will not be removed from a degraded sample. Use highest quality RNA possible. | |||
| Fungus (Basidiomycete) | Use highest quality RNA possible. | |||
| Fungus (Candida albicans) | ||||
| Fungus (Cryptococcus neoformans) | Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible. | |||
| Fungus (Yarrowia lipolytica) | Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible. | |||
| Horse (Equine) | ||||
| Human | All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples. | |||
| Insect (Rhodnius prolixus) | Use highest quality RNA possible. Mitochondrial rRNA not removed. | |||
| Lamprey | Use highest quality RNA possible. | |||
| Marine sponge (Crambe crambe) | Use highest quality RNA possible. | |||
| Monkey (Macaque; Cynomolgus macaques) | ||||
| Monkey (African green; Chlorocebus sabaeus) | ||||
| Monkey (Rhesus; Macaca mulatta) | ||||
| Mosquitoes (Anopheles gambiae) | Removes 18S rRNA. Will not remove 5S, 5.8S, and 12S, 16S mitochondrial rRNA. 28S rRNA not tested. | |||
| Mouse | All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples. | |||
| Nematode (C. elegans) | Use highest quality RNA possible. | |||
| Newt (Notophthalmus viridescens) | May not remove 5.8S or 5S rRNAs. Use highest quality RNA possible. | |||
| Plasmodium falciparum | Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible. | |||
| Poeciliid fishes | Removes 28S and 18S rRNA. May not remove all 5S rRNA. | |||
| Rat | All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples. | |||
| Salmon (Salmo salar) | Removes 18S, 5.8S, and 5S rRNAs. Will not remove 28S rRNA. Mitochondrial rRNAs were not evaluated. | |||
| Sea anemone (Nematostella vectensis) | Use highest quality RNA possible. | |||
| Swine | Removes 28S, 18S, 5.8S and 5S rRNAs. | |||
| Toxoplasma gondii | Use high quality RNA. | |||
| Trypanosoma brucei | Will remove 28S and 18S rRNA. Other rRNA were not evaluated. | |||
| Welsh Onion (Allium fistulosum) | Removes 18S rRNA. Other rRNAs not tested. | |||
| Zebrafish (Danio rerio) |
| Organism | Laboratory Tested | In silico Tested | Customer Reported | Comments |
|---|---|---|---|---|
| Human | Blood samples only; for all other tissues use TruSeq Stranded Total RNA-with Ribo-Zero Human/Mouse/Rat or TruSeq Stranded Total RNA-with Ribo-Zero Gold | |||
| Mouse | Blood samples only; for all other tissues use TruSeq Stranded Total RNA-with Ribo-Zero Human/Mouse/Rat or TruSeq Stranded Total RNA-with Ribo-Zero Gold | |||
| Rat | Blood samples only; for all other tissues use TruSeq Stranded Total RNA-with Ribo-Zero Human/Mouse/Rat or TruSeq Stranded Total RNA-with Ribo-Zero Gold |
| Organism | Laboratory Tested | In silico Tested | Customer Reported | Comments |
|---|---|---|---|---|
| Arabidopsis | Compatible with leaves, seeds and roots. | |||
| Chickpea (Cicer arietinum) | Removes 4.5S, 16S, 23S chloroplast rRNA and 18S cytoplasmic rRNA. Will not remove 5S chloroplast and 5S cytoplasmic rRNA. Other rRNAs not tested. | |||
| Corn | Compatible with leaves, seeds and roots. | |||
| Cotton | ||||
| Eukaryotic green algae (Chlamydomonas) | Use highest quality RNA possible. | |||
| Grapevine | ||||
| Japanese honeysuckle (Lonicera japonica) | ||||
| Maize | Compatible with leaves, seeds and roots. | |||
| Mirror bush (Coprosma repens) | ||||
| Moss (Physcomitrella) | ||||
| Prorocentrum minimum | Removes 18S and 28S rRNA. Other rRNAs not tested. | |||
| Rice | Compatible with leaves, seeds and roots. | |||
| Soybean | Compatible with leaves, seeds and roots. | |||
| Tomato (Solanum lycopersicum) | ||||
| Vietnamese coriander (Persicaria odorata) | ||||
| Water clover (Marsilea vistita) | ||||
| Welsh onion | Use highest quality RNA possible. | |||
| Wheat | Compatible with leaves, seeds and roots. |
Analyze both coding RNA and multiple forms of noncoding RNA for a comprehensive view of the transcriptome.
Find out more about how library preparation works, explore solutions, and get technical tips.
Identify sequencing panels or microarrays that target your gene(s) of interest.