AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel

Targeted RNA research panel to investigate T cell diversity and clonal expansion by sequencing T cell receptor beta chain rearrangements.

5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)

Assay time

< 1.5 hr

Hands-on time

See full details in the specifications table

Overview

AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel is a highly multiplexed targeted resequencing panel to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. Use a single pool of multiplex PCR primers, library reagents, and sample barcodes, to generate libraries from RNA extracted from whole blood, fresh-frozen tissue, or FACS-sorted cells.

Key features

  • High-value content - Obtain comprehensive coverage of TCR beta chain sequence

  • Fast, streamlined workflow - Prepare sequencing-ready libraries in a single day from whole blood, fresh/frozen tissue, or fluorescence-activated cell sorting (FACS) cells

  • Low input requirement - Generate high-quality sequencing libraries from as little as 10 ng of input RNA

The Immune Repertoire Plus, TCR beta Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS), NGS technology, and automated analysis.

This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.


Specifications


Required products

The AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel workflow requires the Immune Response Panel oligos, one of the AmpliSeq Library PLUS kits, an AmpliSeq cDNA Synthesis kit, and at least one AmpliSeq index kit.

/ Results

Applications

Example workflow


Project recommendations

Instrument Recommended number of samples Read length
MiniSeq System

Samples per run will depend on sample type.

2 × 150 bp

MiSeq System

Samples per run will depend on sample type.

2 x 300 bp

NextSeq 550 System

Samples per run will depend on sample type.

2 × 150 bp


Related applications and methods

Compare

AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel AmpliSeq for Illumina TCR beta-SR Panel
Assay time 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5.5~7.5時間(時間はライブラリー調製のみを表し、ライブラリー定量、ノーマライゼーション、プーリングは含まない)
Content specifications RNA evaluation of TCRβ chain rearrangements, including CDR1, CDR2, and CDR3 (with up to 400 bp read-length amplicons) Sequences TCR beta chain rearrangements, with up to 80 bp read-length amplicons for characterizing CDR3.
Hands-on time < 1.5 hr <1.5 hr
インプット量 10~1,000 ng 1 ng
Instruments MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiniSeq System MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiniSeq System
Mechanism of action Multiplex PCR Multiplex PCR
Method Amplicon sequencing, Targeted RNA sequencing Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing
Nucleic acid type RNA DNA, RNA
Sample type details

Libraries can be generated from RNA extracted from whole blood, fresh/frozen tissue, or fluorescence-activated cell sorting (FACS)-sorted cells.

Specialized sample types Blood Blood, FFPE tissue
Species category Human Human
Technology Sequencing Sequencing
Variant class Transcript variants Transcript variants

Figures

High-quality sequencing data that aligns to T cell clonotypes

Panel (1)

AmpliSeq™ for Illumina® Immune Repertoire Plus, TCR beta Panel

20024479

Sufficient for 24 reactions when paired with AmpliSeq Library PLUS for Illumina. Purchase library prep, index adapters, and accessory products separately.

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Library prep (3)

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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Index adapters (6)

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set A for Illumina®

20019105

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set B for Illumina®

20019106

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set C for Illumina®

20019107

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set D for Illumina®

20019167

Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.

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Accessory products (2)

Ampliseq™ cDNA Synthesis for Illumina®

20022654

Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels).

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AmpliSeq™ Library Equalizer for Illumina®

20019171

Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.

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