TruSeq RNA Library Prep Kit v2

These kits provide a simple, cost-effective solution for analysis of the coding transcriptome, with minimal hands-on time. Read More...
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TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes)



TruSeq RNA Library Preparation Kit v2, Set B (48 samples, 12 indexes)



Product Highlights

Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simple workflow with master-mixed reagents.

Multiplex Capabilities

Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples. The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process. This allows the samples to be pooled and then individually identified during downstream analysis.

Minimal Hands-On Steps

Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with a user-friendly workflow.

Fast, automation-friendly workflow

Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.

Frequently Purchased Together


Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System RNA Profiling: 13–40 samples per run (based on 10 million reads per sample)
Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample)
2 x 75 bp

Product Comparison

TruSeq RNA Library Prep Kit v2 Illumina Stranded mRNA Prep Illumina RNA Prep with Enrichment
Assay Time ~10.5 hours 6.5 hours < 9 hours
Content Specifications Captures the coding transcriptome (without strand information) Captures the coding transcriptome with strand information Captures the coding transcriptome when used with Illumina Exome Panel
Description A simple, cost-effective research solution for analysis of the coding transcriptome. A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information A reproducible, economical solution enabling targeted transcript detection and discovery from a broad range of sample types and inputs including formalin-fixed, paraffin- embedded (FFPE) tissues and other low-quality samples
Hands-On Time ~4.5 hours < 3 hours < 2 hours
Input Quantity 0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails) 25-1000 ng standard-quality total RNA 10ng total RNA from fresh/frozen samples, or 20ng total RNA from FFPE samples
Mechanism of Action Oligo-dT beads capture polyA tails PolyA capture, ligation-based addition of adapters and indexes Bead-linked transposome
Method mRNA Sequencing mRNA Sequencing mRNA Sequencing, Target Enrichment, Targeted RNA Sequencing
Multiplexing Up to 24-plex per lane Up to 384 Unique Dual Indexes (UDIs) Up to 384 Unique Dual Indexes (UDIs)
Specialized Sample Types Not FFPE-Compatible Low-Input Samples, Not FFPE-Compatible Blood, FFPE Tissue, Low-Input Samples
Species Category Bovine, Human, Mammalian, Mouse, Rat Bovine, Human, Mammalian, Mouse, Rat Human, Virus
Strand Specificity Non-Stranded Stranded Non-Stranded

Method-Specific Workflow Example


Customer Stories

The Epigenetic Patterns of Exercise

Methylation analysis and RNA-Seq are helping researchers understand how endurance training makes epigenetic changes to the human genome.

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Supporting Data and Figures

TruSeq RNA Library Preparation Reagents Provide Significant Savings in Time and Effort
TruSeq RNA Library Prep Reagents number of steps

Compared to current methods for preparing mRNA samples for sequencing, use of the TruSeq reagents significantly reduces the number of steps and hands-on time.

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Determine the best library prep kit or array for your needs based on your starting material and method of interest.

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