Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simple workflow with master-mixed reagents.
Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples. The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process. This allows the samples to be pooled and then individually identified during downstream analysis.
Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with a user-friendly workflow.
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| NextSeq 550 System | RNA Profiling: 13–40 samples per run (based on 10 million reads per sample) Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample) |
2 x 75 bp |
| TruSeq RNA Library Prep Kit v2 | Illumina Stranded mRNA Prep | Illumina RNA Prep with Enrichment | |
|---|---|---|---|
| Assay Time | ~10.5 hours | 6.5 hours | < 9 hours |
| Content Specifications | Captures the coding transcriptome (without strand information) | Captures the coding transcriptome with strand information | Captures the coding transcriptome when used with Illumina Exome Panel |
| Description | A simple, cost-effective research solution for analysis of the coding transcriptome. | A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information | A reproducible, economical solution enabling targeted transcript detection and discovery from a broad range of sample types and inputs including formalin-fixed, paraffin- embedded (FFPE) tissues and other low-quality samples |
| Hands-On Time | ~4.5 hours | < 3 hours | < 2 hours |
| Input Quantity | 0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails) | 25-1000 ng standard-quality total RNA | 10ng total RNA from fresh/frozen samples, or 20ng total RNA from FFPE samples |
| Mechanism of Action | Oligo-dT beads capture polyA tails | PolyA capture, ligation-based addition of adapters and indexes | Bead-linked transposome |
| Method | mRNA Sequencing | mRNA Sequencing | mRNA Sequencing, Target Enrichment, Targeted RNA Sequencing |
| Multiplexing | Up to 24-plex per lane | Up to 384 Unique Dual Indexes (UDIs) | Up to 384 Unique Dual Indexes (UDIs) |
| Specialized Sample Types | Not FFPE-Compatible | Low-Input Samples, Not FFPE-Compatible | Blood, FFPE Tissue, Low-Input Samples |
| Species Category | Bovine, Human, Mammalian, Mouse, Rat | Bovine, Human, Mammalian, Mouse, Rat | Human, Virus |
| Strand Specificity | Non-Stranded | Stranded | Non-Stranded |
Methylation analysis and RNA-Seq are helping researchers understand how endurance training makes epigenetic changes to the human genome.
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Compared to current methods for preparing mRNA samples for sequencing, use of the TruSeq reagents significantly reduces the number of steps and hands-on time.
TruSeq RNA and DNA Library Preparation Kits v2
Data Sheet | PDF < 1 MB
Illumina Experiment Manager User Guide Documentation
TruSeq Sample Prep Best Practices Guide Documentation
Sequencing Library qPCR Quantification Guide Documentation
TruSeq RNA Sample Prep v2 Guide Documentation
TruSeq RNA Library Prep Kit v2 Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
Determine the best library prep kit or array for your needs based on your starting material and method of interest.
Access Tool
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
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Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with exceptional study flexibility.