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TruSeq Small RNA Library Preparation Kits

These kits provide a simple, cost-effective solution for generating miRNA and small RNA sequencing libraries directly from total RNA, for any species.

1 day

Assay time

4 hr

Hands-on time

See full details in the specifications table

Reagent and Library Prep Kit Bundles Available for the MiniSeq System
Order your MiniSeq reagents and library prep kits together for additional savings and convenience. Select any of item numbers 20005613 through 20005616, and add to your cart.

Overview

TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. Compatible applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the data In BaseSpace Sequence Hub:

View run (primary analysis and metrics)

View project (secondary analysis with BaseSpace Apps)

Specifications

Assay time 1 day
Automation capability Liquid handling robots
Hands-on time 4 hr
Instruments MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiniSeq System, NextSeq 500 System
Mechanism of action Captures small RNA with 3′ OH and 5′P modifications (like those processed by Dicer/Drosha)
Method miRNA and small RNA sequencing, mRNA sequencing
Multiplexing 48 unique indexes are available
Nucleic acid type RNA
Specialized sample types Low-input samples
Species category Drosophila, Any species, Mouse, Zebrafish, Human, Rat, Plant, Nematode
Technology Sequencing
Variant class Novel transcripts, Transcript variants

Applications

Example workflow

1
Prepare
2
Sequence
3
Analyze

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Documentation

Product literature

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Support documentation

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Library prep (8)

TruSeq Small RNA Library Prep Kit -Set A (24 rxns) (Set A: indexes 1-12)

RS-200-0012

Includes reagents for library preparation of 24 small RNA samples for multiplexed sequencing. Each kit contains indexes 1–12 of 48 unique indexes.

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TruSeq Small RNA Library Prep Kit -Set B (24 rxns) (Set B: indexes 13-24)

RS-200-0024

Includes reagents for library preparation of 24 small RNA samples for multiplexed sequencing. Each kit contains indexes 13–24 of 48 unique indexes.

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TruSeq Small RNA Library Prep Kit -Set C (24 rxns) (Set C: indexes 25-36)

RS-200-0036

Includes reagents for library preparation of 24 small RNA samples for multiplexed sequencing. Each kit contains indexes 25–36 of 48 unique indexes.

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TruSeq Small RNA Library Prep Kit -Set D (24 rxns) (Set D: indices 37-48)

RS-200-0048

Includes reagents for library preparation of 24 small RNA samples for multiplexed sequencing. Each kit contains indexes 37–48 of 48 unique indexes.

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TruSeq Small RNA Set A MiniSeq Kit (1 library prep and 2 MiniSeq reagents)

20005613

Kitted reagents for library preparation of 24 small RNA samples for multiplexed sequencing on the MiniSeq System. Each kit contains indexes 1-12 of 48 unique indexes. Bundle includes 2 High-Output 75 cycle MiniSeq Reagent Kits.

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TruSeq Small RNA Set B MiniSeq Kit (1 library prep and 2 MiniSeq reagents)

20005614

Kitted reagents for library preparation of 24 small RNA samples for multiplexed sequencing on the MiniSeq System. Each kit contains indexes 13-24 of 48 unique indexes. Bundle includes 2 High-Output 75 cycle MiniSeq Reagent Kits.

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TruSeq Small RNA Set C MiniSeq Kit (1 library prep and 2 MiniSeq reagents)

20005615

Kitted reagents for library preparation of 24 small RNA samples for multiplexed sequencing on the MiniSeq System. Each kit contains indexes 25-36 of 48 unique indexes. Bundle includes 2 High-Output 75 cycle MiniSeq Reagent Kits.

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TruSeq Small RNA Set D MiniSeq Kit (1 library prep and 2 MiniSeq reagents)

20005616

Kitted reagents for library preparation of 24 small RNA samples for multiplexed sequencing on the MiniSeq System. Each kit contains indexes 37-48 of 48 unique indexes. Bundle includes 2 High-Output 75 cycle MiniSeq Reagent Kits.

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