TruSight Cardio Sequencing Panel

This kit provides researchers with a comprehensive, cost-effective solution for identifying causal variants implicated in inherited cardiac conditions (ICCs).

TruSight Cardio enrichment oligos are now available separately for use with Illumina DNA Prep with Enrichment. The TruSight Cardio Sequencing Kit for MiSeq and MiSeqDx (FC-141-1010) and TruSight Cardio Sequencing Kits for NextSeq (FC-141-1011 and 20035190) have been discontinued.

The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.

Overview

The TruSight Cardio Panel uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart Centre Singapore and Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.1

  • Expertly defined genes selected in collaboration with the Imperial College of London

  • 99% of the targeted regions covered at a depth of at least 20×*

  • Cost to sequence is ~ $1 US per gene; fully supported on Illumina sequencing and informatics platforms

* Data on file. Illumina, Inc. 2015.


Specifications

Applications

Example workflow


Project recommendations

Instrument Recommended number of samples Read length
MiSeq System

12 samples per run with v2 reagents (based on 300x mean coverage of targeted content)

2 × 150 bp (max recommended)

NextSeq 550 System

96 samples per run (mid output; based on 300x mean coverage of targeted content)

2 × 150 bp (max recommended)


Related applications and methods

Compare

TruSight Cardio Sequencing Panel TruSight One Sequencing Panels
Content specifications 575 Kb genomic content (174 genes) TruSight One: ~12 Mb genomic content (~4800 genes).
TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes).
Description Targeted sequencing research panel to identify causal variants associated with 17 inherited cardiac conditions.
Input quantity 50 ng DNA 50 ng DNA
Instruments MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System, NovaSeq 6000 System
Method Targeted DNA sequencing, Target enrichment Targeted DNA sequencing, Target enrichment
Multiplexing Up to 96-plex Up to 96-plex
Nucleic acid type DNA DNA
Specialized sample types Not FFPE-Compatible Not FFPE-Compatible
Species category Human Human
Technology Sequencing Sequencing
Variant class Single nucleotide polymorphisms (SNPs), Germline variants Single nucleotide polymorphisms (SNPs), Germline variants

Resources

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Dr. Stuart Cook: Targeted gene analysis with TruSight Cardio Sequencing

Download this reference guide to understand which kit quantities you should purchase to support your research.

Enrichment panels (1)

TruSight Cardio – Enrichment Oligos only (8 Enrichment Reactions)

20029229

Sufficient for eight enrichment reactions when paired with Illumina DNA Prep with Enrichment. Purchase library prep and enrichment reagents and index adapters separately.

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Library prep (4)

Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)

20025523

Includes reagents for preparing and enriching 16 libraries (16, 1-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.

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Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)

20025524

Includes reagents for preparing and enriching 96 libraries (eight, 12-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.

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Illumina® DNA Prep, (S) Tagmentation (16 Samples)

20025519

Includes reagents for preparing 16 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (16 samples)).

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Illumina® DNA Prep, (S) Tagmentation (96 Samples)

20025520

Includes reagents for preparing 96 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (96 samples)).

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Accessory products (4)

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)

20027215

IDT for Illumina- Nextera UD Indexes- Set C contains 96, 10 bp unique indexes sufficient for 96 samples. Library prep, enrichment, and enrichment probe panel reagents need to be ordered separately.

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)

20027216

IDT for Illumina- Nextera UD Indexes- Set D contains 96, 10 bp unique indexes sufficient for 96 samples. Library prep, enrichment, and enrichment probe panel reagents need to be ordered separately.

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Selection summary

Product

Qty

Unit Price

Order the NovaSeq X Series

Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.

MiSeq Reagent Kit v3

Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

References

1. Pua CJ, Bhalshankar J, Miao K, et al. Development of a comprehensive sequencing assay for inherited cardiac condition genes. J Cardiovasc Trans Res. 2016;9:3.

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