NextSeq 1000/2000 Reagents

Reagents for the NextSeq 1000/2000 System feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.

Overview

NextSeq 1000/2000 reagents leverage XLEAP-SBS chemistry to enable a wide range of applications.

These reagents offer:

  • Sequencing output of 10–540 Gb per run, providing adjustable output based on project needs

  • Multiple flow cell configurations that offer flexibility to support new and emerging applications

Sustainability and simplicity in one

Easy-to-use reagent cartridges deliver a streamlined workflow that reduces the chance for errors, lowers operating costs, improves recyclability, and minimizes waste. The fully-integrated cartridges include reagents, fluidics, and the waste holder, streamlining library loading and instrument use. 

Simply prepare the reagent cartridge, insert the flow cell, load the library into the cartridge, and insert into the instrument. 


Required products

Use XLEAP-SBS P1 reagents on both the NextSeq 1000 and NextSeq 2000 Systems. Maximum output: 60 Gb. Maximum single-end reads per run: 100M.

Use XLEAP-SBS P2 reagents on both the NextSeq 1000 and NextSeq 2000 Systems. Maximum output: 240 Gb. Maximum single-end reads per run: 400M. 

Use XLEAP-SBS P3 reagents on the NextSeq 2000 System only. Maximum output: 360 Gb. Maximum single-end reads per run: 1.2B.

Use XLEAP-SBS P4 reagents on the NextSeq 2000 System only. Maximum output: 540 Gb. Maximum single-end reads per run: 1.8B.

The NextSeq 1000/2000 Index Primer Kit, Read Primer Kit, and Read and Index Primers are optional but required when both custom sequencing primers and Illumina primers are used in a run.* 

PhiX Control v3 is recommended for all runs, but is optional for well-balanced library types. 

/ Results

*The XLEAP-SBS cartridge already contains the primers for Illumina DNA PCR-Free Prep, whereas the standard SBS cartridge does not.

Documentation

Product literature

XLEAP-SBS reagents (17)

NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (600 Cycles)

20100981

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 600 cycles of sequencing, plus dual-indexing support (up to 100M single reads).

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NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (300 Cycles)

20100982

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 100M single reads).

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NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (100 Cycles)

20100983

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 100M single reads).

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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (600 Cycles)

20100984

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 600 cycles of sequencing, plus dual-indexing support (up to 400M single reads).

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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (300 Cycles)

20100985

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 400M single reads).

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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (200 Cycles)

20100986

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 400M single reads).

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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (100 Cycles)

20100987

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 400M single reads).

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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (300 Cycles)

20100988

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).

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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (200 Cycles)

20100989

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).

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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (100 Cycles)

20100990

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).

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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (300 Cycles)

20100992

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (200 Cycles)

20100993

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (100 Cycles)

20100994

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (50 Cycles)

20100995

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 50 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 1000/2000 XLEAP-SBS™ Read and Index Primer Kit

20112856

Reagents to utilize custom read and index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge. This kit is sufficient for 1 custom read primer and 1 custom index primer use.

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NextSeq™ 1000/2000 XLEAP-SBS™ Index Primer Kit

20112858

Reagents to utilize custom index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge. This kit is sufficient for 10 custom index primer uses.

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NextSeq™ 1000/2000 XLEAP-SBS™ Read Primer Kit

20112859

Reagents to utilize custom read primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge. This kit is sufficient for 10 custom read primer uses.

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Standard SBS reagents (11)

NextSeq™1000/2000 P1 Reagents(100cycles)

20074933

Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (100 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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