TruSight Oncology 500 High-Throughput

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI) from FFPE tissue, with added scalability.

4–5 days from sample to results

Assay time

~2.5 hr for automated workflow ~10.5 hr for manual workflow

Hands-on time

40 ng DNA and/or 40–80 ng RNA

Input quantity

See full details in the specifications table

Overview

TruSight Oncology 500 High-Throughput (HT) is a highly scalable pan-cancer, next-generation sequencing (NGS) assay that enables in-house comprehensive genomic profiling (CGP) from FFPE tissue.  

  • Targets 523 genes to assess all DNA and RNA variant types (SNV, CNV, indels, fusions), plus MSI and TMB 

  • Delivers accurate results with hybrid–capture chemistry and sophisticated bioinformatics 

  • Reduces hands-on time and increases scalability with optional automation kits and methods*

  • Accommodates 16–192 samples per flow cell

Meaningful results

Evaluate > 500 pan-cancer biomarkers aligned with guidelines and clinical trials to increase chances of finding a positive biomarker with a single assay.  

Streamlined workflow

Get results in 4–5 days using manual or automated workflows integrated with ready-to-use DRAGEN secondary analysis pipelines via on-premises server or in the cloud with Illumina Connected Analytics. Access an integrated interpretation report through Illumina Connected Insights or Pierian. 

* Automation available on the Beckman Coulter iSeries or the Hamilton Microlab STAR. Contact Illumina Sales for more information.

Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources. 


Enable CGP with a comprehensive portfolio

Identify relevant biomarkers for clinical research with the TruSight Oncology 500 product line. 

TruSight Oncology 500 High-Throughput

Batch up to 192 samples at a time while using the same panel content and tissue input type as TruSight Oncology 500.

‡ HRD is only available with the addition of the TruSight Oncology 500 HRD kit to TruSight Oncology 500. Not available in Japan. 

§NovaSeq 6000Dx System in Research Mode requires a separate, stand-alone DRAGEN server for secondary analysis. 


Specifications


Required products

To run TruSight Oncology 500 High-Throughput (TSO 500 HT) you need:

To analyze with the DRAGEN variant calling pipeline you need: 

To generate a final report, you need:

§NovaSeq 6000Dx System in Research Mode requires a separate, stand-alone DRAGEN server for secondary analysis.

†Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.

/ Results

Applications

TruSight Oncology 500 High-Throughput enables comprehensive genomic profiling of FFPE tumor samples to assess a wide range of biomarkers at increased scalability, from 16–192 samples per flow cell.

Example workflow

** Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.


Project recommendations

Instrument Recommended number of samples Read length
NovaSeq 6000 System

16 samples per run (SP flow cell), 100M paired-end reads, 3,500× coverage 

32 samples per run (S1 flow cell), 100M paired-end reads, 3,500× coverage 

72 samples per run (S2 flow cell), 100M paired-end reads, 3,500× coverage 

192 samples per run (S4 flow cell), 100M paired-end reads, 3,500× coverage 

2 × 101 bp 

NovaSeq 6000Dx in Research Mode

16 samples per run (SP flow cell), 100M paired-end reads, 3,500× coverage

32 samples per run (S1 flow cell), 100M paired-end reads, 3,500× coverage

72 samples per run (S2 flow cell), 100M paired-end reads, 3,500× coverage

192 samples per run (S4 flow cell), 100M paired-end reads, 3,500× coverage

2 × 101 bp


Related applications and methods

Documentation

Product literature

Compare

TruSight Oncology 500 High-Throughput TruSight Oncology 500 TruSight Oncology 500 ctDNA v2
Assay time 4–5 days from sample to results 4–5 days from sample to results 3-4 days from purified nucleic acid to variant report
Automation capability Liquid handling robots Liquid handling robots
Automation details Explore available automation methods Explore available automation methods
Cancer type Pan-cancer, Solid tumor Pan-cancer, Solid tumor Pan-cancer, Solid tumor
Content specifications Targeted sequencing of DNA from 523 genes of interest and RNA from 55 genes, for a total of 1.94Mb panel size. MSI and TMB measurement included.  Targeted sequencing of DNA from 523 genes and RNA from 55 genes for a total of 1.94 Mb panel size. MSI and TMB measurement included.  The optional TruSight Oncology 500 HRD kit (not available in Japan) content includes coverage of ~25K SNPs to assess homologous recombination deficiency through a comprehensive genomic instability score (LOH+TAI+LST) powered by Myriad Genetics.  Targeted selection of 523 genes (full coding sequence) for a total of 1.94Mb panel size.
• Immuno-oncology biomarker coverage: TMB and MSI
• Guideline coverage: Broad coverage of key guidelines for multiple solid tumor types
• Clinical trial coverage: Over 600 clinical trials (based on Pierian clinical knowledgebase, as of February 2023)
Description A high-throughput comprehensive NGS assay to identify key biomarkers in guidelines and > 1K clinical trials from a streamlined workflow using the NovaSeq 6000 System or NovaSeq 6000Dx Instrument (in Research Mode). Includes coverage of immuno-oncology biomarkers MSI and TMB. Assay that enables comprehensive genomic profiling from FFPE tissue and runs on the NextSeq 550 System or NextSeq 550Dx Instrument (in Research Mode) and can batch up to eight samples at a time. Provides a noninvasive method for profiling solid tumors for cancer research applications through comprehensive genomic profiling of liquid biopsy samples (ctDNA from blood plasma). This liquid biopsy approach provides insights about intra- and inter-tumor heterogeneity using a minimally invasive sample collection approach to complement tissue-based CGP.
Hands-on time ~2.5 hr for automated workflow
~10.5 hr for manual workflow
~2.5 hr for automated workflow
~10.5 hr for manual workflow
~1.5-2.5 hr for library prep and enrichment
Input quantity 40 ng DNA and/or 40–80 ng RNA 40 ng DNA and/or 40 ng RNA 20 ng cfDNA (4 ml of plasma)
Instruments NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System NextSeq 550 System, NextSeq 550Dx in Research Mode, NextSeq 500 System NovaSeq 6000 System
Method Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment Targeted DNA sequencing, Target enrichment
Multiplexing Up to 16-plex (SP flow cell), 32-plex (S1 flow cell), 72-plex (S2 flow cell), and 192-plex (S4 flow cell) Up to 8-plex Up to 8 on S2, and 24 on S4 using Xp-4 Lane workflow, 16 indexes maximum
Nucleic acid type DNA, RNA DNA, RNA DNA
Sample throughput 16–192 samples per run
Specialized sample types FFPE tissue FFPE tissue Circulating tumor DNA, Blood
Species category Human Human Human
Technology Sequencing Sequencing Sequencing
Variant class Gene fusions, Somatic variants, Novel transcripts, Structural variants, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) Gene fusions, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs), Loss of heterozygosity (LOH), Somatic variants, Novel transcripts, Single nucleotide polymorphisms (SNPs), Structural variants Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs)

Figures

High concordance of TMB status between DRAGEN TruSight Oncology v2 and Local App

High concordance of MSI status between DRAGEN TruSight Oncology v2 and Local App

High concordance of CNV (gene amplification) detection between DRAGEN TruSight Oncology v2 and Local App

Robust detection of fusions and splice variants

The information in this table shows examples of concordance between TruSight Oncology 500 and TruSight Oncology 500 High-Throughput and is not a comprehensive list of the fusions and splice variants detected. These data were generated with a Local App pipeline (not DRAGEN software).

DRAGEN brings speed to TruSight Oncology 500 secondary analysis

Resources

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Automation with TruSight Oncology 500 HT

Hear how customers at Intermountain Precision Genomics enabled CGP and scaled up their workflow with automation. 

Manual prep (8)

TruSight Oncology 500 DNA High-Throughput (48 Samples)

20040765

Includes reagents for preparing and enriching up to 48 DNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.

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TruSight Oncology 500 DNA High-Throughput (144 Samples)

20040767

Includes reagents for preparing and enriching up to 144 DNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.

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TruSight Oncology 500 DNA/RNA High-Throughput (24 Samples)

20040764

Includes reagents for preparing and enriching up to 24 DNA and RNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.

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TruSight Oncology 500 DNA/RNA High-Throughput (72 Samples)

20040766

Includes reagents for preparing and enriching up to 72 DNA and RNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.

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TruSight Oncology 500 DNA/RNA High-Throughput Kit (24 Samples), plus Velsera

20040768

Includes reagents for preparing and enriching up to 24 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.

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TruSight Oncology 500 DNA High-Throughput Kit (48 Samples), plus Velsera

20040769

Includes reagents for preparing and enriching up to 48 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.

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List Price:

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TruSight Oncology 500 DNA/RNA High-Throughput Kit (72 Samples), plus Velsera

20040770

Includes reagents for preparing and enriching up to 72 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.

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List Price:

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TruSight Oncology 500 DNA High-Throughput Kit (144 Samples), plus Velsera

20040771

Includes reagents for preparing and enriching up to 144 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.

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Automation (8)

TruSight Oncology 500 High-Throughput DNA for Automation (64 Samples)

20049283

Includes reagents for preparing and enriching up to 64 DNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.

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TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples)

20049282

Includes reagents for preparing and enriching up to 32 DNA and RNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.

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