TruSight Oncology 500 High-Throughput (HT) is a highly scalable pan-cancer, next-generation sequencing (NGS) assay that enables in-house comprehensive genomic profiling (CGP) from FFPE tissue.
TruSight Oncology 500 High-Throughput
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI) from FFPE tissue, with added scalability.
4–5 days from sample to results
Assay time
~2.5 hr for automated workflow ~10.5 hr for manual workflow
Hands-on time
40 ng DNA and/or 40–80 ng RNA
Input quantity
Now supported on the NovaSeq X Series, NextSeq 1000 and NextSeq 2000
Overview
Meaningful results
Evaluate > 500 pan-cancer biomarkers aligned with guidelines and clinical trials to increase chances of finding a positive biomarker with a single assay.
Streamlined workflow
Get results in 4–5 days using manual or automated workflows integrated with ready-to-use DRAGEN secondary analysis pipelines via on-premises server or in the cloud with Illumina Connected Analytics. Access an integrated interpretation report through Illumina Connected Insights† or Velsera Clinical Genomics Workspace (CGW).
* Automation available on the Beckman Coulter iSeries or the Hamilton Microlab STAR. Contact Illumina Sales for more information.
†Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.
Enable CGP with a comprehensive portfolio
Identify relevant biomarkers for clinical research with the TruSight Oncology 500 product line.
TruSight Oncology 500 High-Throughput
Batch up to 960 samples at a time while using the same panel content and tissue input type as TruSight Oncology 500.
Assess relevant DNA and RNA cancer biomarkers from FFPE tumor tissue, now including HRD through an accessory kit to evaluate the Genomic Instability Score (GIS).‡
Analyze circulating tumor DNA (ctDNA) in blood plasma via liquid biopsy with similar DNA panel content as TruSight Oncology 500.
‡ HRD is only available with the addition of the TruSight Oncology 500 HRD kit to TruSight Oncology 500. Not available in Japan.
§ NextSeq 1000, NextSeq 2000, NovaSeq 6000Dx System (in research mode) and NovaSeq X Series require a separate, stand-alone DRAGEN server for secondary analysis.
Specifications
| Assay time | 4–5 days from sample to results |
|---|---|
| Automation capability | Liquid handling robot(s) |
| Automation details | Explore available automation methods |
| Cancer type | Pan-cancer, Solid tumor |
| Content specifications | Targeted sequencing of DNA from 523 genes of interest and RNA from 55 genes, for a total of 1.94Mb panel size. MSI and TMB measurement included. |
| Description | A high-throughput comprehensive NGS assay to identify key biomarkers in guidelines and >1K clinical trials from a streamlined workflow using the NextSeq 1000, NextSeq 2000, NovaSeq 6000, NovaSeq 6000Dx (in research mode) or the NovaSeq X Series. Includes coverage of immuno-oncology biomarker TMB and MSI. |
| Hands-on time |
~2.5 hr for automated workflow ~10.5 hr for manual workflow |
| Input quantity | 40 ng DNA and/or 40–80 ng RNA |
| Instruments | NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System |
| Method | Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment |
| Multiplexing |
NextSeq 1000 and 2000: P2 flow cell 8 samples, P3 flow cell 24 samples, P4 flow cell 36 samples NovaSeq 6000/Dx: SP flow cell 16 samples, S1 flow cell 32 samples, S2 flow cell 72 samples, S4 flow cell 192 samples NovaSeq X Series*: 1.5B cartridge 32 samples, 10B cartridge 192 samples, 25B 480 samples |
| Nucleic acid type | DNA, RNA |
| Sample throughput | NextSeq 1000 and 2000: 8-36 samples/run, NovaSeq 6000/Dx: 16-192 samples/run, NovaSeq X: 32-480 samples/run, NovaSeq X Plus: 32-960 samples/run |
| Specialized sample types | FFPE tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Gene fusions, Somatic variants, Novel transcripts, Structural variants, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) |
*Throughput shown is for the single flow cell NovaSeq X Instrument. NovaSeq X Plus offers a dual flow cell system with twice the capacity listed.
Required products
To run TruSight Oncology 500 High-Throughput (TSO 500 HT) you need:
- NextSeq 1000 and 2000, NovaSeq 6000 System, NovaSeq 6000Dx Instrument (in Research Mode)§ or NovaSeq X Series
- A TSO 500 HT library prep kit with a manual or automated workflow (DRAGEN variant calling pipeline included)
- NextSeq 1000 and 2000 XLEAP-SBS reagent kit with three flow cell options OR NovaSeq 6000 v1.5 reagent kit with four flow cell options OR NovaSeq X reagent kit with three flow cell options depending on throughput needs
- Index adapters for a manual or automated workflow (sold separately)
To analyze with the DRAGEN variant calling pipeline you need:
- A DRAGEN server license for local analysis, or
- An Illumina Connected Analytics subscription and iCredits for cloud analysis
To generate a final report, you need:
§NextSeq 1000, NextSeq 2000, NovaSeq 6000Dx (in research mode) and NovaSeq X Series require a separate, stand-alone DRAGEN server for secondary analysis.
†Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.
/
Results
Applications
TruSight Oncology 500 High-Throughput enables comprehensive genomic profiling of FFPE tumor samples to assess a wide range of biomarkers at increased scalability, from 8–480 samples per flow cell.
Example workflow
Prep
TruSight Oncology 500 High-Throughput assay
Analyze
** Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.
Project recommendations
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| NextSeq 1000 System, NextSeq 2000 System | 8 samples per run (P2 flow cell), 800M paired-end reads, 3,500× coverage 24 samples per run (P3 flow cell), 2.4B paired-end reads, 3,500× coverage 36 samples per run (P4 flow cell), 3.6B paired-end reads, 3,500× coverage |
2 x 101 bp |
| NovaSeq 6000 System | 16 samples per run (SP flow cell), 100M paired-end reads, 3,500× coverage 32 samples per run (S1 flow cell), 100M paired-end reads, 3,500× coverage 72 samples per run (S2 flow cell), 100M paired-end reads, 3,500× coverage 192 samples per run (S4 flow cell), 100M paired-end reads, 3,500× coverage |
2 × 101 bp |
| NovaSeq 6000Dx in Research Mode | 16 samples per run (SP flow cell), 100M paired-end reads, 3,500× coverage 32 samples per run (S1 flow cell), 100M paired-end reads, 3,500× coverage 72 samples per run (S2 flow cell), 100M paired-end reads, 3,500× coverage 192 samples per run (S4 flow cell), 100M paired-end reads, 3,500× coverage |
2 × 101 bp |
| NovaSeq X System | 32 samples per run (1.5B flow cell), 3.2B paired-end reads, 3,500× coverage 192 samples per run (10B flow cell), 20B paired-end reads, 3,500× coverage 480 samples per run (25B flow cell), 52B paired-end reads, 3,500× coverage *Throughput shown is for the single flow cell NovaSeq X Instrument. |
2 × 101 bp |
Related applications and methods
Reduce hands-on time and minimize errors with high- and low-throughput walk-away automation protocols available through Illumina automation partners.
Next-generation sequencing enables immuno-oncology researchers to gain insights into immunotherapy response factors and tumor immune evasion mechanisms.
Pathology and clinical cancer research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.
Documentation
Product literature
Support documentation
- DRAGEN TruSight Oncology 500 Analysis Software v2.1 Documentation
- DRAGEN TruSight Oncology 500 Analysis Software v2.1.2 on ICA Documentation
- TruSight Oncology 500 v1.0 Local App Documentation
- TruSight Oncology 500 High Throughput Consumables & Equipment Documentation
- DRAGEN TruSight Oncology 500 Analysis Software Online Help (v2.5.2 and later) Documentation
- TruSight Oncology 500 v2.1 Local App Documentation
- DRAGEN TruSight Oncology 500 Analysis Software v1.1 on ICA Documentation
- Infinium Global Clinical Research Array Documentation
- TruSight Oncology 500 High Throughput Checklist Documentation
- DRAGEN TruSight Oncology 500 Analysis Software v1.1 Documentation
- TruSight Oncology 500 High Throughput Reference Guide Documentation
- TruSight Oncology 500 v2.0 Local App Documentation
- TruSight Oncology 500 v2.2 Local App Documentation
- Reference guide Documentation
Compare
| TruSight Oncology 500 High-Throughput | TruSight Oncology 500 | TruSight Oncology 500 ctDNA v2 | |||
|---|---|---|---|---|---|
| Assay time | 4–5 days from sample to results | 4–5 days from sample to results | 3-4 days from purified nucleic acid to variant report | ||
| Automation capability | Liquid handling robot(s) | Liquid handling robot(s) | Liquid handling robot(s) | ||
| Automation details | Explore available automation methods | Explore available automation methods | Explore available automation methods | ||
| Cancer type | Pan-cancer, Solid tumor | Pan-cancer, Solid tumor | Pan-cancer, Solid tumor | ||
| Content specifications | Targeted sequencing of DNA from 523 genes of interest and RNA from 55 genes, for a total of 1.94Mb panel size. MSI and TMB measurement included. | Targeted sequencing of DNA from 523 genes and RNA from 55 genes for a total of 1.94 Mb panel size. MSI and TMB measurement included. The optional TruSight Oncology 500 HRD kit (not available in Japan) content includes coverage of ~25K SNPs to assess homologous recombination deficiency through a comprehensive genomic instability score (LOH+TAI+LST) powered by Myriad Genetics. |
Targeted selection of 523 genes (full coding sequence) for a total of 1.94Mb panel size. • Immuno-oncology biomarker coverage: TMB and MSI • Guideline coverage: Broad coverage of key guidelines for multiple solid tumor types • Clinical trial coverage: Over 600 clinical trials (based on Velsera clinical knowledgebase, as of February 2023) |
||
| Description | A high-throughput comprehensive NGS assay to identify key biomarkers in guidelines and >1K clinical trials from a streamlined workflow using the NextSeq 1000, NextSeq 2000, NovaSeq 6000, NovaSeq 6000Dx (in research mode) or the NovaSeq X Series. Includes coverage of immuno-oncology biomarker TMB and MSI. | Assay that enables comprehensive genomic profiling from FFPE tissue and runs on the NextSeq 550 System or NextSeq 550Dx Instrument (in Research Mode) and can batch up to eight samples at a time. | Provides a noninvasive research method for comprehensive genomic profiling of liquid biopsy samples (ctDNA from blood plasma). This liquid biopsy approach provides insights about intra- and inter-tumor heterogeneity using a minimally invasive sample collection approach to complement tissue-based CGP. | ||
| Hands-on time |
~2.5 hr for automated workflow ~10.5 hr for manual workflow |
~2.5 hr for automated workflow ~10.5 hr for manual workflow |
~1.5 hr for automated workflow ~2.5 hr for manual workflow |
||
| Input quantity | 40 ng DNA and/or 40–80 ng RNA | 40 ng DNA and/or 40 ng RNA | 20 ng cfDNA (4 ml of plasma) | ||
| Instruments | NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System | NextSeq 550 System, NextSeq 550Dx in Research Mode, NextSeq 500 System | NovaSeq X System, NovaSeq 6000 System | ||
| Method | Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment | Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment | Targeted DNA sequencing, Target enrichment | ||
| Multiplexing |
NextSeq 1000 and 2000: P2 flow cell 8 samples, P3 flow cell 24 samples, P4 flow cell 36 samples NovaSeq 6000/Dx: SP flow cell 16 samples, S1 flow cell 32 samples, S2 flow cell 72 samples, S4 flow cell 192 samples NovaSeq X Series*: 1.5B cartridge 32 samples, 10B cartridge 192 samples, 25B 480 samples |
Up to 8-plex |
NovaSeq X Series: 4 samples on 1.5B flow cell, 24 samples on 10B flow cell NovaSeq 6000 System: 8 samples on S2 flow cell, 24 samples on S4 flow cell, 192 indexes maximum |
||
| Nucleic acid type | DNA, RNA | DNA, RNA | DNA | ||
| Sample throughput | NextSeq 1000 and 2000: 8-36 samples/run, NovaSeq 6000/Dx: 16-192 samples/run, NovaSeq X: 32-480 samples/run, NovaSeq X Plus: 32-960 samples/run | 8 samples/run | 4-48 samples/run | ||
| Specialized sample types | FFPE tissue | FFPE tissue | Circulating tumor DNA, Blood | ||
| Species category | Human | Human | Human | ||
| Technology | Sequencing | Sequencing | Sequencing | ||
| Variant class | Gene fusions, Somatic variants, Novel transcripts, Structural variants, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) | Gene fusions, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs), Loss of heterozygosity (LOH), Somatic variants, Novel transcripts, Single nucleotide polymorphisms (SNPs), Structural variants | Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) |
Figures
High concordance of TMB status between DRAGEN TruSight Oncology v2 and Local App
High concordance of MSI status between DRAGEN TruSight Oncology v2 and Local App
High concordance of CNV (gene amplification) detection between DRAGEN TruSight Oncology v2 and Local App
Robust detection of fusions and splice variants
The information in this table shows examples of concordance between TruSight Oncology 500 and TruSight Oncology 500 High-Throughput and is not a comprehensive list of the fusions and splice variants detected. These data were generated with a Local App pipeline (not DRAGEN software).
DRAGEN brings speed to TruSight Oncology 500 secondary analysis
CNV (Gene Amplification) Concordance
High concordance between NovaSeq X Series and NovaSeq 6000 for TSO 500 HT
Resources
Automation with TruSight Oncology 500 HT
Hear how customers at Intermountain Precision Genomics enabled CGP and scaled up their workflow with automation.
Manual prep (9)
TruSight Oncology 500 DNA High-Throughput (48 Samples)
20040765
Includes reagents for preparing and enriching up to 48 DNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
List Price:
Discounts:
TruSight Oncology 500 DNA High-Throughput (144 Samples)
20040767
Includes reagents for preparing and enriching up to 144 DNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
List Price:
Discounts:
TruSight Oncology 500 DNA/RNA High-Throughput (24 Samples)
20040764
Includes reagents for preparing and enriching up to 24 DNA and RNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
List Price:
Discounts:
TSO 500 DNA/RNA HT (24 Spl) plus Illumina Connected Insights Software
20119460
Includes library prep and enrichment reagents plus data interpretation reports (through ICI).
List Price:
Discounts:
TruSight Oncology 500 DNA/RNA High-Throughput (72 Samples)
20040766
Includes reagents for preparing and enriching up to 72 DNA and RNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
List Price:
Discounts:
TruSight Oncology 500 DNA/RNA High-Throughput Kit (24 Samples), plus Velsera
20040768
Includes reagents for preparing and enriching up to 24 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
List Price:
Discounts:
TruSight Oncology 500 DNA High-Throughput Kit (48 Samples), plus Velsera
20040769
Includes reagents for preparing and enriching up to 48 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
List Price:
Discounts:
TruSight Oncology 500 DNA/RNA High-Throughput Kit (72 Samples), plus Velsera
20040770
Includes reagents for preparing and enriching up to 72 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
List Price:
Discounts:
TruSight Oncology 500 DNA High-Throughput Kit (144 Samples), plus Velsera
20040771
Includes reagents for preparing and enriching up to 144 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
List Price:
Discounts:
Automation (9)
TruSight Oncology 500 High-Throughput DNA for Automation (64 Samples)
20049283
Includes reagents for preparing and enriching up to 64 DNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
List Price:
Discounts:
TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples)
20049282
Includes reagents for preparing and enriching up to 32 DNA and RNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
List Price:
Discounts:
TSO500 DNA/RNA HT Auto (32 spls)+ plus Illumina Connected Insights Software
20119461
Includes library prep and enrichment reagents plus data interpretation reports (through ICI).
List Price:
Discounts:
TruSight Oncology 500 High-Throughput DNA for Automation (64 Samples), Plus Velsera
20049277
Includes reagents for preparing and enriching up to 64 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
List Price:
Discounts:
TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples), Plus Velsera
20049276
Includes reagents for preparing and enriching up to 32 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
List Price:
Discounts:
TruSight Oncology 500 High-Throughput DNA for Automation (144 Samples)
20049285