Viral Surveillance Panel
Viral Surveillance Panel offers whole-genome sequencing and characterization of 66 viruses including SARS-CoV-2, influenza, and polio with target enrichment.
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Using the Viral Surveillance Panel with Illumina RNA Prep with Enrichment kit allows researchers to obtain whole genome sequencing (WGS) data that can characterize 66 viruses that are of high risk to public health, including SARS-CoV-2, Influenza, Monkeypox Virus, and Poliovirus. The design allows for proactive, broad pathogen surveillance. Hybrid capture panels allow for variation in viral genomes, allowing for mutations and novel strains to be detected.
Target Enrichment for Virus Detection
Target enrichment through a hybrid–capture method allows for whole genome sequencing of multiple viruses, without requiring the high read depth needed for shotgun metagenomic sequencing. WGS of multiple viruses concurrently allows for understanding of viral evolution and broad viral surveillance.1
Compared to other targeted resequencing methods, such as amplicon sequencing, enrichment through hybrid capture allows for larger and tiled probe panels with more comprehensive characterization of multiple viruses at once and difficult to sequence genomes. Additionally, the oligo probes used for hybrid–capture protocols remain effective even within highly mutagenic regions, allowing targeting of rapidly evolving viruses, such as RNA viruses.
Streamlined Workflow
This comprehensive workflow allows researchers and public health scientists to monitor multiple high-risk viruses (both DNA & RNA) with one panel/workflow. This workflow integrates library preparation, target enrichment, sequencing, and data analysis. The workflow is intended to enrich whole viral genomes from total nucleic acid extraction. Corresponding data analysis can be completed with the DRAGEN Microbial Enrichment app.
1. Gaudin M and Desnues C. Hybrid Capture-Based Next Generation Sequencing and Its Application to Human Infectious Diseases. Front Microbiol. 2018; 9:2924.
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Specifications
Product Comparison
| Viral Surveillance Panel | Illumina Respiratory Virus Enrichment Kit | Respiratory Pathogen ID/AMR Enrichment Panel Kit | |
|---|---|---|---|
| Assay Time | < 9 hours library prep time | < 9 hours library prep time | < 9 hours library prep time |
| Automation Capability | Liquid Handling Robots | Liquid Handling Robots | Liquid Handling Robots |
| Description | A streamlined workflow for whole-genome sequencing and analyzing 66 viruses that are high risk to public health including, including SARS-CoV-2, Influenza, Monkeypox Virus, and Poliovirus using RNA Prep with Enrichment combined with Viral Surveillance Panel for library prep, whole-genome-sequencing, and simplified data analysis using the DRAGEN Microbial Enrichment App. | A streamlined workflow for detecting and analyzing SARS-CoV-2 and other common respiratory viruses using Illumina RNA Prep with Enrichment reagents combined with the Respiratory Virus Oligo Panel v2 for library prep and enrichment, Illumina sequencing, and simplified data analysis using the DRAGEN Microbial Enrichment App. | A streamlined workflow using Respiratory Pathogen ID/AMR Panel (RPIP) with Illumina RNA Prep with Enrichment, sequencing on Illumina desktop systems, and powerful analysis using Explify RPIP Software allows researchers to identify respiratory infections and co-infections, identify antimicrobial resistance, and perform strain typing of critical pathogens (SARS-CoV-2 and Flu A/B viruses) to study viral evolution and transmission. |
| Hands-On Time | < 2 hours library prep time | < 2 hours library prep and enrichment time | < 2 hours library prep and enrichment time |
| Input Quantity | 10-100 ng | 10-100 ng | 10-100 ng |
| Mechanism of Action | On-Bead Tagmentation followed by a single hybridization step | On-Bead Tagmentation followed by a single hybridization step | On-Bead Tagmentation followed by a single hybridization step |
| Method | Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing, Whole-Genome Sequencing | Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing | Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing |
| Multiplexing | Up to 384 samples in a single run with unique dual indexes | Up to 384 samples in a single run with unique dual indexes | Up to 384 samples in a single run with unique dual indexes |
| Nucleic Acid Type | RNA, DNA | RNA, DNA | RNA, DNA |
| Species Category | Virus | Human, Virus | Bacteria, Fungal, Virus |
| Technology | Sequencing | Sequencing | Sequencing |
Product Literature
Data Sheet | PDF | 7 versions
Manuals and Support Information
Illumina RNA Prep with Enrichment (L) Tagmentation Documentation
Illumina RNA Prep Tagmentation (L) with Enrichment - Protocol Checklist Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
Illumina RNA Prep with Enrichment
Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.
Respiratory Pathogen ID/AMR Enrichment Panel Kit
This NGS panel targets >280 respiratory pathogens (including SARS-CoV-2) and 2000+ antimicrobial resistance alleles, with data analysis powered by Explify RPIP.
Illumina Respiratory Virus Enrichment Kit
Highly sensitive detection and characterization of common respiratory viruses, including SARS-CoV-2 strains, with comprehensive, rapid target enrichment sequencing.