Contact your local Illumina Support team to access the Local UMI Error Correction App.
To enable accurate detection of rare variants, UMIs are integrated with error correction software, enabling true mutations to be distinguished from background noise. The bottom panel illustrates how, by removing inherent errors that result in false positives, the reduced error rate allows true mutations (red dots) to be better distinguished from background noise (grey dots).
|Sequencing Run||Mean Error Rate (Uncollapsed Reads)||Mean Error Rate (Collapsed Reads)|
Library preparation was performed using the TruSight Oncology UMI Reagents paired with DNA content from the TruSight Tumor 170 DNA assay, and 31 samples were distributed among four independent sequencing runs on the HiSeq 4000 System. Mean error rates are shown with and without collapsed reads using the UMI Error Correction App.
* Performance on the NovaSeq 6000 System was demonstrated but not extensively tested.