AmpliSeq for Illumina On-Demand Panels

Select from a catalog of > 5000 pretested genes to create custom targeted resequencing panels

Scientists reviewing Ampliseq on computer screen

Design DNA or RNA panels with pretested gene content

Confidently tailor panel designs for human disease research by selecting from over 5000 genes relevant for inherited disease research, including hereditary cancer, primary immunodeficiency, hearing loss, muscular dystrophy, and more. All genes have been pretested on the NextSeq 550 Sequencing System.

Use the free web-based DesignStudio Assay Design Tool to add up to 500 genes of interest and order your panel in convenient small packs of 24 or 96 reactions.

Featured AmpliSeq for Illumina products

AmpliSeq for Illumina On-Demand

Tailor panel designs for human disease research efficiently and conveniently by selecting from a catalog of over 5,000 pretested genes.

AmpliSeq for Illumina Custom DNA Panel  

Targeted custom research panels optimized for sequencing specific targets or genomic content of interest.

AmpliSeq for Illumina Custom RNA Panel

Targeted custom RNA research panels optimized for sequencing up to 1200 genomic targets of interest.

Key features and benefits

Simplified panel creation

With the simple user interface, robust algorithms, and dynamic feedback provided by DesignStudio, On-Demand Panels can be created easily.

Pretested content

Fuel your study with optimized and tested content in numerous genetic disease research areas.

Content flexibility

Design panels with 1–500 genes and 24–15,000 amplicons.

Streamlined data analysis

Perform secondary analysis workflows in the cloud via BaseSpace Sequence Hub or on-instrument via Local Run Manager.

Low-input sample types

Obtain high-quality data with high coverage uniformity even with minimal input from challenging sample types like formalin-fixed, paraffin-embedded (FFPE) tissue.

Fast library prep

Prepare an on-target, high-uniformity amplified library in as little as 5 hours with just 1.5 hours of hands-on time.

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How AmpliSeq for Illumina On-Demand Panels work

To create your AmpliSeq for Illumina On-Demand Panel, use DesignStudio Assay Design Tool to select parameters like assay type, species, sample type, and maximum amplicon length. Add your targets using genes or coordinates of interests. Receive, review, and adjust your design before ordering your custom PCR-based library prep assay.

Libraries are prepared using multiplexed PCR amplification of your targets prior to sequencing on an Illumina next-generation sequencing (NGS) system using sequencing by synthesis (SBS) chemistry. You can analyze data on the cloud with the DRAGEN Amplicon pipeline or on-instrument with Local Run Manager to get accurate results without extensive bioinformatics resources.

Learn more about DesignStudio Assay Design Tool

AmpliSeq for Illumina On-Demand Panel workflow

Select content

User-friendly assay design:

Prep

AmpliSeq for Illumina Library Prep, Index Adapters, and Accessories

Sequence

Benchtop sequencers

Analyze

Custom assay design services

Improve coverage and enable add-on or non-human species designs with Illumina Concierge design support. Our Concierge team provides end-to-end project management, in-silico coverage, and design assistance.

Request pricing

AmpliSeq for Illumina On-Demand Panel FAQ

Sign up for or log in to DesignStudio Assay Design Tool to start a new design. Select a preloaded reference genome, then add in your genomic regions of interest. If you like the content from AmpliSeq for Illumina Ready-to-Use or Community Panels, you can directly upload Product Manifest Files into the DesignStudio tool as a starting point for further customization. Learn more about .

You can pool up to 96 samples per sequencing run with integrated sample barcodes. The actual number of samples that can be pooled depends on the number of amplicons and the desired depth of coverage. DesignStudio Assay Design Tool features an online calculator to inform these parameters.

AmpliSeq for Illumina On-Demand Panels are tailored panels for germline analysis in human disease research, selected from a catalog of over 5000 pre-tested genes. AmpliSeq for Illumina Custom Panels are fully personalized and optimized content for your targets of interest, for germline or somatic analysis. You can start with your custom target list, or you can modify an AmpliSeq for Illumina Ready-to-Use or Community Panel.

Yes, all > 5000 available genes have been tested in an actual sequencing run on the NextSeq 550 Sequencing System, allowing you to confidently select the content you need for your research.

The includes a step-by-step guide to using the DesignStudio Assay Design Tool. Contact Illumina Technical Support if you need further assistance with DesignStudio. If you need help with ordering, contact a sales representative.

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Contact an Illumina specialist to learn more about AmpliSeq for Illumina products.