Targeted research panel investigating hotspot regions of 50 genes with known associations to cancer.
Assay time
Hands-on time
Input quantity
The AmpliSeq for Illumina Cancer Hotspot Panel v2 is a targeted resequencing assay for researching somatic mutations across the hotspot regions of 50 genes with known associations to cancer, as identified in the Catalogue of Somatic Mutations in Cancer (COSMIC)1 database.
The Cancer HotSpot Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This panel enables highly sensitive variant detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| Assay time | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
|---|---|
| Cancer type | Pan-Cancer, Solid Tumor |
| Content specifications | Hotspot regions of 50 genes with known associations to cancer |
| Description | Somatic analysis research into hotspot regions of 50 cancer-related genes. |
| Hands-on time | < 1.5 hr |
| Input quantity | 1–100 ng (10 ng recommended per pool) |
| Instruments | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System |
| Mechanism of action | Multiplex PCR |
| Method | Amplicon Sequencing, Targeted DNA Sequencing |
| Multiplexing | 96 dual index combinations |
| Nucleic acid type | DNA |
| Number of amplicons | 207 total. 1 pool. |
| Specialized sample types | FFPE Tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Somatic variants, Insertions-deletions (indels) |
The AmpliSeq for Illumina Cancer Hotspot Panel workflow requires the Cancer Hotspot Panel, Library PLUS kit, and index adapter.
AmpliSeq for Illumina Cancer HotSpot Panel v2
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| iSeq 100 System | 16 samples per run (assumes minimum coverage of 500×) |
2 × 150 bp |
| MiniSeq System | Samples per run: mid output: 32, high output: 96 (assumes minimum coverage of 500×) |
2 × 150 bp |
| MiSeq System | Samples per run (by reagent kit version): v2 nano: 4, v2 micro: 16, v2: 60, v3: 96 (assumes minimum coverage of 500×) |
2 × 150 bp |
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
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Our sequencing and microarray technologies support a broad range of cancer genomics research applications, from DNA to RNA analysis, epigenetics, and more.
| AmpliSeq for Illumina Cancer Hotspot Panel v2 | AmpliSeq for Illumina Focus Panel | AmpliSeq for Illumina Comprehensive Panel v3 | TruSight Tumor 15 | |
|---|---|---|---|---|
| Assay time | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) | 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) | 7 hr |
| Cancer type | Pan-Cancer, Solid Tumor | Solid Tumor | Solid Tumor | Pan-Cancer, Solid Tumor |
| Content specifications | Hotspot regions of 50 genes with known associations to cancer | DNA and RNA targets for 52 oncogenes | DNA and RNA targets for 161 oncogenes | Amplifies 250 amplicons from 15 genes associated with solid tumors |
| Description | Somatic analysis research into hotspot regions of 50 cancer-related genes. | Somatic analysis research on 52 genes associated with solid tumor cancers. | Somatic analysis research on hotspot and full-length targets of genes* associated with solid tumor cancers. |
NGS assay for 15 commonly mutated genes in solid tumors using 20 ng DNA. |
| Hands-on time | < 1.5 hr | < 1.5 hr | <1.5 hr | 3.5 hr |
| Input quantity | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) |
20 ng FFPE DNA |
| Instruments | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode | MiSeq System, MiSeqDx in Research Mode, MiniSeq System |
| Mechanism of action | Multiplex PCR | Multiplex PCR | Multiplex PCR | |
| Method | Amplicon Sequencing, Targeted DNA Sequencing | Targeted RNA Sequencing, Amplicon Sequencing, Targeted DNA Sequencing | Targeted RNA Sequencing, Amplicon Sequencing, Targeted DNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing |
| Multiplexing | 96 dual index combinations | 96 dual index combinations | 96 dual index combinations | 1-24-plex |
| Nucleic acid type | DNA | DNA, RNA | DNA, RNA | DNA |
| Number of amplicons | 207 total. 1 pool. | 553 total. 2 pools. (DNA Pool: 269 amplicons. RNA Pool: 284 amplicons.) | 4,648 total 4 pools (DNA pool 1: 1,891 amplicons. DNA pool 2: 1,890 amplicons. RNA pool 1: 447 amplicons. RNA pool 2: 420 amplicons.) | |
| Specialized sample types | FFPE Tissue | FFPE Tissue | FFPE Tissue | FFPE Tissue |
| Species category | Human | Human | Human | Human |
| Technology | Sequencing | Sequencing | Sequencing | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Somatic variants, Insertions-deletions (indels) | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs) | Somatic variants, Insertions-deletions (indels) |
AmpliSeq™ Cancer HotSpot Panel v2 for Illumina®
20019161
Targeted panel for assessing somatic mutations across hotspot regions of 50 cancer-related genes. Kit contains 24 reactions. Library PLUS and index adapters sold separately.
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AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set B for Illumina®
20019106
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set C for Illumina®
20019107
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.
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AmpliSeq™ for Illumina® Sample ID Panel
20019162
Includes eight single nucleotide polymorphism (SNP)-targeting primer pairs and one gender discriminating primer pair sufficient for 96 reactions when paired with AmpliSeq Library PLUS for Illumina. Enables quick and accurate sample identification. Purchase library prep, probe panels, and index adapters separately.
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AmpliSeq™ for Illumina® Direct FFPE DNA
20023378
Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification.
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AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.
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