Illumina RNA Prep with Enrichment
以卓越的捕获效率和覆盖度均一性实现对大量靶基因的快速靶向检测。Read More...
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Illumina RNA Library Prep with Enrichment Training
20044763
*此优惠仅适用于文库制备试剂盒，不包括标签接头和panel。
Illumina RNA Library Prep with Enrichment Training
20044763
Illumina RNA Prep with Enrichment采用On-Bead Tagmentation技术和简化的单一杂交实验，可为基于RNA的文库制备和富集提供超快速的工作流程。
Learn more about the technology
总时间不到9小时，制备和富集文库的手动操作时间不到2小时。与TruSeq RNA Exome相比，此工作流程至少可节省50%的时间。我们将通过自动化合作伙伴提供Illumina认证的自动化选项。
仅需10 ng来自新鲜或冷冻样本的总RNA，或20 ng来自降解样本（例如FFPE）的总RNA即可实现高灵敏度。
获取目标转录组编码区，只需中度测序深度即可获取大量信息。Illumina RNA Prep with Enrichment可提供出色的捕获效率和覆盖度均一性，极大地减少所需的测序深度，实现无偏差的准确检测。每个富集反应只需2.5 µL探针。
您可以从各种经过验证的预先构建的探针池中选择，也可以自定义自己的探针以获得更大的灵活性。使用新的唯一双标签组C和D（即将推出），一次运行可以对384个样本进行多重分析。模块化设计可对目标区域开展多种RNA-Seq应用，从而进行检测和发现研究，例如等位基因特异性表达、融合检测、生物标志物筛选等。
实现对大量靶基因的快速靶向检测，包括RNA外显子组。Illumina RNA Prep with Enrichment可与Illumina Exome Panel一起使用，该panel的探针组经过了高度的优化，可全面覆盖编码RNA序列。Illumina Exome Panel包含超过425,000个探针，每个都根据NCBI37/hg19参考基因组构建，覆盖了98%以上的RefSeq外显子组。此组探针可捕获超过214,000个目标，涵盖了21,415个目的基因。
Respiratory Virus Oligo Panel可对常见的呼吸道病毒（包括冠状病毒株）进行高度灵敏的检测和鉴定。即使在高度变异区域，用于基于杂交捕获的靶向富集的寡核苷酸探针仍然有效，能靶向快速进化的RNA病毒。Respiratory Virus Oligo Panel与Illumina RNA Prep with Enrichment一起使用。
了解更多| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NextSeq 550 System | Illumina RNA Prep with Enrichment + Exome Panel
中通量: 5 高通量: 16 (基于每个样本25M read) |
2 × 100 bp |
| NextSeq 2000 System | Illumina RNA Prep with Enrichment + Exome Panel
P2: 16 P3: 40 (基于每个样本25M read) |
2 × 100 bp |
| NovaSeq 6000 System | Illumina RNA Prep with Enrichment + Exome Panel
SP: 32 S1: 64 S2: 164 S4: 384 (基于每个样本25M read) |
2 × 100 bp |
| MiSeq System, MiSeq | Illumina RNA Prep with Enrichment + Respiratory Virus Oligo Panel
Up to 25 samples (基于每个样本1M read) |
2 × 75 bp |
| MiniSeq System | Illumina RNA Prep with Enrichment + Respiratory Virus Oligo Panel
Up to 25 samples (基于每个样本1M read) |
2 × 75 bp |
| NextSeq 550 System | Illumina RNA Prep with Enrichment + Respiratory Virus Oligo Panel
Up to 384 samples (基于每个样本1M read) |
2 × 75 bp |
| Illumina RNA Prep with Enrichment | TruSeq RNA Exome | Illumina Stranded mRNA Prep | |
|---|---|---|---|
| Assay Time | ＜9小时 | 约两天 | 6.5小时 |
| Automation Capability | 液体处理机器人 | 液体处理机器人 | 液体处理机器人 |
| Content Specifications | 与Illumina Exome Panel一起使用时可捕获编码转录组 | 捕获编码转录组/RNA外显子组 | 捕获编码转录组（带链信息） |
| Description | 一种可重现且经济的解决方案，可使用多种类型的样本和起始材料来检测和发现目标转录本，包括福尔马林固定石蜡包埋（FFPE）组织和其他低质量样本 | 为研究人员提供清晰、全面的编码转录组视图，链信息精确且样本起始量低。 | 简单、经济的编码转录组分析解决方案，可提供精确的链信息 |
| Hands-On Time | ＜2小时 | 约11小时 | ＜3小时 |
| Input Quantity | 10 ng新鲜/冷冻样本来源的总RNA，或20 ng FFPE样本来源的总RNA | 10 ng新鲜/冷冻样本来源的总RNA，或20 ng FFPE样本来源的总RNA | 25-1000 ng标准质量的总RNA |
| Mechanism of Action | 连接磁珠的转座酶 | 以编码RNA为靶标的生物素化的捕获探针。不要求RNA带有poly-A尾巴。 | PolyA捕获，连接接头和标签 |
| Method | mRNA Sequencing , 靶向RNA测序 , 靶向富集 | Exome Sequencing , mRNA Sequencing | mRNA Sequencing |
| Multiplexing | 多达384个唯一双标签序列（UDI） | 多达24个单标签、96个组合（CD）双标签 | 多达384个唯一双标签序列（UDI） |
| Technology | 测序 | 测序 | 测序 |
| Variant Class | Novel Transcripts, Transcript Variants, 单核苷酸多态性 (SNPs), 基因融合, 转录变异 | Novel Transcripts, Transcript Variants, 单核苷酸多态性 (SNPs), 转录变异 | Novel Transcripts, Transcript Variants, 单核苷酸多态性 (SNPs), 基因融合, 转录变异 |
On-Bead Tagmentation and a single, 90-minute hybridization step combine to deliver a faster workflow with fewer steps compared to TruSeq RNA Exome.
Illumina RNA Prep with Enrichment achieves high data concordance between input amounts of 10 ng and 100 ng total RNA from universal human reference (UHR). UHR RNA libraries were sequenced on a NovaSeq 6000 System, subsampled to 25M clusters per library. Data was analyzed with the BaseSpace RNA-Seq Alignment v 1.1.1 app.
Libraries prepared from 10 ng RNA from the K-562 cell line using Illumina RNA Prep with Enrichment and the Illumina Exome Panel resulted in successful detection of the BCR-ABL1 gene fusion. Top alignment track shows all reads, bottom track shows only reads supporting the BCR-ABL1 fusion gene.
| Fusion (source) | RIN | RNA input | Detection |
|---|---|---|---|
| BCR-ABL 1 (K-562) | 7.4 | 10 ng | 6/6 replicates (100%) |
| TPM3-NTRK1 (colorectal cancer) | 2.5 | 20 ng | 6/6 replicates (100%) |
Libraries prepared from 10 ng UHR RNA and 20 ng FFPE RNA using Illumina RNA Prep with Enrichment and the Illumina Exome Panel show more than 85% of the data generated aligned to transcripts (coding and UTRs). Data from TruSeq RNA Exome libraries are shown for comparison.
Application Note | PDF< 1 MB
Application Note | PDF< 1 MB
Improved detection of circulating transcripts
Application Note | PDF< 1 MB
Enrichment of coding transcripts in degraded samples using hybrid probe capture
Application Note | PDF< 1 MB
Application Note | PDF< 1 MB
Application Note | PDF< 1 MB
Brochure | PDF1 MB
Illumina library preparation solutions
Brochure | PDF2 MB
Data Sheet | PDF | 6 versions
Illumina RNA Prep with Enrichment (L) Tagmentation Documentation
Illumina RNA Prep with Enrichment (L) Tagmentation Reference Guide Documentation
Illumina RNA Prep with Enrichment (L) Tagmentation Consumables & Equipment Documentation
Illumina RNA Prep with Enrichment (L) Tagmentation Checklist Documentation
自定义协议选择器
Generates customized, end-to-end instructions
全部 Illumina RNA Prep with Enrichment (L) Tagmentation Support
Expert-defined content targeting 113 genes associated with genetic cancer risk predisposition.
