TruSeq RNA Exome, previously known as the TruSeq RNA Access Library Prep Kit, converts total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissues and other low-quality samples.
TruSeq RNA Exome generates RNA sequencing (RNA-Seq) libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions to help maximize discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis. Reagent volumes supplied are sufficient to support 1- to 4-plex enrichment reactions.
Read a comprehensive assessment of Illumina’s ribosomal RNA depletion protocol
Instrument | Recommended Number of Samples | Read Length |
---|---|---|
NextSeq 550 System | 5 to 16 samples per run (based on 25 million reads per sample) | 2 x 75 bp |
NovaSeq 6000 System | Samples per run (dual flow cell): S1: 96, S2: 96, S4: 192 (currently limited by available index combinations) | 2 × 100 bp |
NovaSeq 6000 System | Samples per run (dual flow cell): S1: 96, S2: 96, S4: 192 (currently limited by available index combinations) | 2 × 100 bp |
TruSeq RNA Exome | Illumina RNA Prep with Enrichment | Illumina Stranded mRNA Prep | |
---|---|---|---|
Assay Time | ~2 Days | < 9 hours | 6.5 hours |
Automation Capability | Liquid Handling Robots | Liquid Handling Robots | Liquid Handling Robots |
Content Specifications | Captures the coding transcriptome/RNA exome | Captures the coding transcriptome when used with Illumina Exome Panel | Captures the coding transcriptome with strand information |
Description | Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information and a reduced sample input requirement. | A reproducible, economical solution enabling targeted transcript detection and discovery from a broad range of sample types and inputs including formalin-fixed, paraffin- embedded (FFPE) tissues and other low-quality samples | A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information |
Hands-On Time | ~11 hours | < 2 hours | < 3 hours |
Input Quantity | 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples | 10ng total RNA from fresh/frozen samples, or 20ng total RNA from FFPE samples | 25-1000 ng standard-quality total RNA |
Mechanism of Action | Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails. | Bead-linked transposome | PolyA capture, ligation-based addition of adapters and indexes |
Method | Exome Sequencing, mRNA Sequencing | mRNA Sequencing, Target Enrichment, Target Enrichment, Targeted RNA Sequencing | mRNA Sequencing |
Multiplexing | Up to 24 single, 96 combinatorial (CD) dual | Up to 384 Unique Dual Indexes (UDIs) | Up to 384 Unique Dual Indexes (UDIs) |
Specialized Sample Types | FFPE Tissue, Low-Input Samples | Blood, FFPE Tissue, Low-Input Samples | Low-Input Samples, Not FFPE-Compatible |
Species Category | Human | Human, Virus | Bovine, Human, Mammalian, Mouse, Rat |
Strand Specificity | Stranded | Non-Stranded | Stranded |
The product previously known as the TruSeq RNA Access Library Prep Kit (Cat. No. RS-301-2001 and RS-301-2002) is now called TruSeq RNA Exome. The product configuration has changed. In the new configuration, major components such as library preparation, index adapters, enrichment reagents, and exome panels can be purchased separately. If you were previously buying Cat. No. RS-301-2001 or Cat. No. RS-301-2002, purchase the following components: TruSeq RNA Library Prep for Enrichment (Cat. No. 20020189), TruSeq RNA Enrichment (Cat. No. 20020490), Exome Panel (Cat. No. 20020183), and one item from the Index Adapters section below.
Researchers rely on transcriptomics to uncover multiple drug-susceptible tumorigenic pathways.
Learn MoreA critical comparison between two popular TruSeq RNA Library Prep Kits reveals new information of interest to researchers conducting RNA sequencing studies.
Read InterviewData Sheet | PDF | 5 versions
RNA-Seq Offers Significant Advantages in Immuno-Oncology Research
White Paper | PDF 2 MB
Evaluating RNA Quality from FFPE Samples
Technical Note | PDF 1 MB
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
TruSeq RNA Exome Support - Documentation & Literature
TruSeq RNA Exome Checklist Documentation
TruSeq RNA Exome Documentation
TruSeq RNA Exome Consumables & Equipment Documentation
TruSeq RNA Exome Reference Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions